Recombinant E. coli BirA protein (Active) is a Escherichia coli K-12 Full Length protein, in the 2 to 321 aa range, expressed in Escherichia coli, with >=65% purity and suitable for SDS-PAGE, WB, FuncS.
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Key facts
- Purity
- >=65% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His-DDDDK tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M H H H H H H D Y K D D D D K L E V L F Q G P K D N T V P L K L I A L L A N G E F H S G E Q L G E T L G M S R A A I N K H I Q T L R D W G V D V F T V P G K G Y S L P E P I Q L L N A E K I L S Q L D D G S V A V L P V I D S T N Q Y L L D R I G E L K S G D A C V A E Y Q H A G R G R R G R K W F S P F G A N L Y L S M F W R L E Q G P A A A I G L S L V I G I V M A E V L R K L G A D K V R V K W P N D L Y L Q D R K L A G I L V E L T G K T G D A A Q I V I G A G I N M A M R R V E E S V V N Q G W I T L Q E A G I N L D R N T L A A M L I R E L R A A L E L F E Q E G L T P Y L S R W E K L D N F I N R P V K L I I G D K E I F G I S R G I D K Q G A L L L E Q D G I I K P W M G G E I S L R S A E K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.
Additional Targets
BirA
Alternative names
bioR, dhbB, b3973, JW3941, birA, Bifunctional ligase/repressor BirA, Biotin operon repressor, Biotin--[acetyl-CoA-carboxylase] ligase, Biotin--protein ligase, Biotin-[acetyl-CoA carboxylase] synthetase
Recommended products
Recombinant E. coli BirA protein (Active) is a Escherichia coli K-12 Full Length protein, in the 2 to 321 aa range, expressed in Escherichia coli, with >=65% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >=65% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His-DDDDK tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- ab135015 (10 μg/ml) completely biotinylated 10 μM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 μM d-Biotin.
- Accession
- P06709-1
- Animal free
- No
- Species
- Escherichia coli K-12
- Concentration
- Storage buffer
pH: 8
Preservative: 1.02% Imidazole
Constituents: 5% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.29% Sodium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M H H H H H H D Y K D D D D K L E V L F Q G P K D N T V P L K L I A L L A N G E F H S G E Q L G E T L G M S R A A I N K H I Q T L R D W G V D V F T V P G K G Y S L P E P I Q L L N A E K I L S Q L D D G S V A V L P V I D S T N Q Y L L D R I G E L K S G D A C V A E Y Q H A G R G R R G R K W F S P F G A N L Y L S M F W R L E Q G P A A A I G L S L V I G I V M A E V L R K L G A D K V R V K W P N D L Y L Q D R K L A G I L V E L T G K T G D A A Q I V I G A G I N M A M R R V E E S V V N Q G W I T L Q E A G I N L D R N T L A A M L I R E L R A A L E L F E Q E G L T P Y L S R W E K L D N F I N R P V K L I I G D K E I F G I S R G I D K Q G A L L L E Q D G I I K P W M G G E I S L R S A E K
- Accession
- P06709
- Protein length
- Full Length
- Predicted molecular weight
- 38 kDa
- Amino acids
- 2 to 321
- Nature
- Recombinant
- Tags
- His-DDDDK tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.
- Sequence similarities
Belongs to the biotin--protein ligase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Useful for covalently biotin-labeling of acceptor proteins for detection and purification assays.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein BirA also known as the biotin protein ligase (BPL) serves an important role in biotinylation. Mechanically BirA activates biotin through ATP-dependent reactions linking it to proteins. The BirA protein has an approximate molecular weight of 35 kDa a characteristic referred to as the BirA molecular weight or BirA protein size. BirA is typically expressed in bacterial cells with Escherichia coli being a primary model organism for studying its activity. In its active form BirA functions as a homodimer a stable complex required for its catalytic activity.
- Biological function summary
BirA adds biotin to specific lysine residues on target proteins a modification important for protein function. This process often regulates proteins involved in metabolism and gene expression. BirA does not operate as a part of a larger protein complex but acts on its own enzyme-substrate interactions. Its distribution is notable in prokaryotic cells where it plays essential roles in microbial biotin metabolism.
- Pathways
BirA significantly interacts in the biotin metabolic pathway vital for fatty acid synthesis and gluconeogenesis. The protein's ability to biotinylate targets is vital in pathways where it helps regulate enzymes like acetyl-CoA carboxylase and pyruvate carboxylase which are critical for lipid and carbohydrate metabolism. In the biotin regulation network BirA interacts with other proteins including the biotin repressor protein BioR for controlling gene expression related to biotin and related pathways.
- Associated diseases and disorders
Deficiencies in biotin metabolism pathways where BirA activity is central can lead to conditions such as biotinidase deficiency and phenylketonuria (PKU). Disruptions in these pathways often result from inadequate biotinylation where BirA plays a critical role. Although not directly linked to human diseases the homologs of BirA studied in human cells are relevant for understanding the molecular basis of metabolic disorders.
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2 product images
SDS-PAGE - Recombinant E. coli BirA protein (Active) (ab135015)
10% SDS-PAGE analysis of ab135015 (4 μg) stained with Coomassie Blue.
Western blot - Recombinant E. coli BirA protein (Active) (ab135015)
MBP-Avi biotinylation with His-FLAG-BirA using the standard reaction conditions as outlined in Enzyme Activity.
A. Coomassie blue stained gel showing protein markers:
1. Unbiotinylated MBP-Avi
2. His-FLAG-BirA / MBP-Avi
3. MBP-Avi-biotin control
B. The corresponding Western blot probed with Streptavidin-HRP and developed with DAB (3,3'-Diaminobenzidine) substrate.All lanes: Western blot - Recombinant E. coli BirA protein (Active) (ab135015)
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