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AB135015

Recombinant E. coli BirA protein (Active) (His-DDDDK tag N-Terminus)

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Recombinant E. coli BirA protein (Active) (His-DDDDK tag N-Terminus) is a Escherichia coli K-12 Full Length protein, in the 2 to 321 aa range, expressed in Escherichia coli, with >65%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

bioR, dhbB, b3973, JW3941, birA, Bifunctional ligase/repressor BirA, Biotin operon repressor, Biotin--[acetyl-CoA-carboxylase] ligase, Biotin--protein ligase, Biotin-[acetyl-CoA carboxylase] synthetase, BioR, dhbB, Bio R, Bir A, Dhb B, Biotin ligase, Biotin protein ligase, Biotin operon repressor, Bifunctional protein birA, BirA bifunctional protein, Biotin [acetyl CoA carboxylase] synthetase

2 Images
Western blot - Recombinant E. coli BirA protein (Active) (AB135015)
  • WB

Unknown

Western blot - Recombinant E. coli BirA protein (Active) (AB135015)

MBP-Avi biotinylation with His-FLAG-BirA using the standard reaction conditions as outlined in Enzyme Activity.

A. Coomassie blue stained gel showing protein markers :
1. Unbiotinylated MBP-Avi
2. His-FLAG-BirA / MBP-Avi
3. MBP-Avi-biotin control

B. The corresponding Western blot probed with Streptavidin-HRP and developed with DAB (3,3'-Diaminobenzidine) substrate.

All lanes:

Western blot - Recombinant E. coli BirA protein (Active) (ab135015)

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SDS-PAGE - Recombinant E. coli BirA protein (Active) (His-DDDDK tag N-Terminus) (AB135015)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli BirA protein (Active) (His-DDDDK tag N-Terminus) (AB135015)

10% SDS-PAGE analysis of ab135015 (4 μg) stained with Coomassie Blue.

Key facts

Purity

>65% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DDDDK tag N-Terminus

Applications

SDS-PAGE, FuncS, WB

applications

Biologically active

Yes

Biological activity

ab135015 (10 μg/ml) completely biotinylated 10 μM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 μM d-Biotin.

Accession

P06709

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

Storage buffer

pH: 8 Preservative: 1.02% Imidazole Constituents: 5% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.29% Sodium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Primary Antibodies

AB232732

Anti-BirA antibody [6C4c7]

Trial Size

Western blot - Anti-BirA antibody [6C4c7] (AB232732)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB106159

Anti-Biotin Ligase Epitope Tag antibody

  • 1
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Useful for covalently biotin-labeling of acceptor proteins for detection and purification assays.
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Sequence info

[{"sequence":"MHHHHHHDYKDDDDKLEVLFQGPKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELKSGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK","proteinLength":"Full Length","predictedMolecularWeight":"38 kDa","actualMolecularWeight":null,"aminoAcidEnd":321,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P06709","tags":[{"tag":"His-DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein BirA also known as the biotin protein ligase (BPL) serves an important role in biotinylation. Mechanically BirA activates biotin through ATP-dependent reactions linking it to proteins. The BirA protein has an approximate molecular weight of 35 kDa a characteristic referred to as the BirA molecular weight or BirA protein size. BirA is typically expressed in bacterial cells with Escherichia coli being a primary model organism for studying its activity. In its active form BirA functions as a homodimer a stable complex required for its catalytic activity.
Biological function summary

BirA adds biotin to specific lysine residues on target proteins a modification important for protein function. This process often regulates proteins involved in metabolism and gene expression. BirA does not operate as a part of a larger protein complex but acts on its own enzyme-substrate interactions. Its distribution is notable in prokaryotic cells where it plays essential roles in microbial biotin metabolism.

Pathways

BirA significantly interacts in the biotin metabolic pathway vital for fatty acid synthesis and gluconeogenesis. The protein's ability to biotinylate targets is vital in pathways where it helps regulate enzymes like acetyl-CoA carboxylase and pyruvate carboxylase which are critical for lipid and carbohydrate metabolism. In the biotin regulation network BirA interacts with other proteins including the biotin repressor protein BioR for controlling gene expression related to biotin and related pathways.

Deficiencies in biotin metabolism pathways where BirA activity is central can lead to conditions such as biotinidase deficiency and phenylketonuria (PKU). Disruptions in these pathways often result from inadequate biotinylation where BirA plays a critical role. Although not directly linked to human diseases the homologs of BirA studied in human cells are relevant for understanding the molecular basis of metabolic disorders.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.

Sequence similarities

Belongs to the biotin--protein ligase family.

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Product protocols

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Target data

Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.
See full target information birA

Additional targets

BirA
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