Recombinant E. coli deoC protein is a Escherichia coli K-12 Full Length protein, in the 1 to 259 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M T D L K A S S L R A L K L M D L T T L N D D D T D E K V I A L C H Q A K T P V G N T A A I C I Y P R F I P I A R K T L K E Q G T P E I R I A T V T N F P H G N D D I D I A L A E T R A A I A Y G A D E V D V V F P Y R A L M A G N E Q V G F D L V K A C K E A C A A A N V L L K V I I E T G E L K D E A L I R K A S E I S I K A G A D F I K T S T G K V A V N A T P E S A R I M M E V I R D M G V E K T V G F K P A G G V R T A E D A Q K Y L A I A D E L F G A D W A D A R H Y R F G A S S L L A S L L K A L G H G D G K S A S S Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate (PubMed:11598300, PubMed:17905878, PubMed:6749498). Can also catalyze the double aldol condensation of three acetaldehyde molecules, leading to the formation of 2,4,6-trideoxyhexose (Ref.5).
Alternative names
Deoxyribose-phosphate aldolase, DERA, 2-deoxy-D-ribose 5-phosphate aldolase, Phosphodeoxyriboaldolase, Deoxyriboaldolase, JW4344, b4381, thyR, dra, deoC
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Recombinant E. coli deoC protein is a Escherichia coli K-12 Full Length protein, in the 1 to 259 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Alternative names
Deoxyribose-phosphate aldolase, DERA, 2-deoxy-D-ribose 5-phosphate aldolase, Phosphodeoxyriboaldolase, Deoxyriboaldolase, JW4344, b4381, thyR, dra, deoC
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- P0A6L0-1
- Animal free
No
- Species
Escherichia coli K-12
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M T D L K A S S L R A L K L M D L T T L N D D D T D E K V I A L C H Q A K T P V G N T A A I C I Y P R F I P I A R K T L K E Q G T P E I R I A T V T N F P H G N D D I D I A L A E T R A A I A Y G A D E V D V V F P Y R A L M A G N E Q V G F D L V K A C K E A C A A A N V L L K V I I E T G E L K D E A L I R K A S E I S I K A G A D F I K T S T G K V A V N A T P E S A R I M M E V I R D M G V E K T V G F K P A G G V R T A E D A Q K Y L A I A D E L F G A D W A D A R H Y R F G A S S L L A S L L K A L G H G D G K S A S S Y
- Accession
- Protein length
Full Length
- Predicted molecular weight
29.9 kDa
- Amino acids
1 to 259
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
ab106864 is purified using conventional chromatography techniques.
General info
- Function
Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate (PubMed:11598300, PubMed:17905878, PubMed:6749498). Can also catalyze the double aldol condensation of three acetaldehyde molecules, leading to the formation of 2,4,6-trideoxyhexose (Ref.5).
- Sequence similarities
Belongs to the DeoC/FbaB aldolase family. DeoC type 2 subfamily.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
- Activity summary
DeoC also known as deoxyribose-phosphate aldolase is an enzyme that plays a mechanical role in catalyzing the cleavage of deoxyribose-5-phosphate into glyceraldehyde-3-phosphate and acetaldehyde. This enzyme weighs approximately 23 kDa. deoC is typically expressed in bacterial cells especially within Escherichia coli where it is involved in DNA metabolism by-products breakdown.
- Biological function summary
The enzyme deoC functions as part of the pentose phosphate pathway critical for cellular functions by contributing to nucleotide synthesis and energy generation. In its role deoC doesn't form part of a larger complex but functions independently. It acts on intermediates in nucleotide salvage pathways therefore playing a role in optimizing cellular resources.
- Pathways
DeoC integrates into the non-oxidative branch of the pentose phosphate pathway facilitating the conversion of sugar phosphates. It interacts with other enzymes like ribose-5-phosphate isomerase and transketolase which are pivotal in these metabolic pathways. These interactions highlight its essential role in connecting nucleotide metabolism and glycolysis.
- Associated diseases and disorders
Researchers have explored deoC's implications in bacterial infections. Its activity significantly impacts the pathogen's survival and virulence influencing disorders related to bacterial persistence. While deoC itself is not directly linked to human diseases it plays an important role in bacterial life cycle and disrupts the metabolic processes that can reduce the bacterial load. This makes it an indirect target for antibiotic strategies.
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SDS-PAGE - Recombinant E. coli deoC protein (ab106864)
15% SDS-PAGE analysis of 3μg ab106864.
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