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AB236935

Recombinant E. coli E. coli heat labile toxin A protein (Tagged)

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Recombinant E. coli E. coli heat labile toxin A protein (Tagged) is a Escherichia coli Full Length protein, in the 19 to 258 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

ltpA, eltA, Heat-labile enterotoxin A chain, LTP-A

1 Images
SDS-PAGE - Recombinant E. coli E. coli heat labile toxin A protein (Tagged) (AB236935)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli E. coli heat labile toxin A protein (Tagged) (AB236935)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab236935.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P06717

Animal free

No

Carrier free

No

Species

Escherichia coli

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"NGDRLYRADSRPPDEIKRSGGLMPRGHNEYFDRGTQMNINLYDHARGTQTGFVRYDDGYVSTSLSLRSAHLAGQSILSGYSTYYIYVIATAPNMFNVNDVLGVYSPHPYEQEVSALGGIPYSQIYGWYRVNFGVIDERLHRNREYRDRYYRNLNIAPAEDGYRLAGFPPDHQAWREEPWIHHAPQGCGNSSRTITGDTCNEETQNLSTIYLREYQSKVKRQIFSDYQSEVDIYNRIRDEL","proteinLength":"Full Length","predictedMolecularWeight":"32.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":258,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P06717","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E. coli heat labile toxin A also known as LT-A is one component of the heat-labile enterotoxin produced by enterotoxigenic Escherichia coli (ETEC). LT-A has a molecular mass of approximately 27 kDa. This toxin consists of two subunits: the enzymatically active A subunit and the pentameric B subunit. Primarily LT-A gets expressed in the intestinal lumen where ETEC colonizes. LT-A is responsible for the ADP-ribosylation of the alpha subunit of G proteins leading to sustained activation of adenylate cyclase.
Biological function summary

E. coli heat labile toxin A plays a significant role in causing traveler's diarrhea by disrupting water and electrolyte absorption in enterocytes. It is part of the ab5 toxin family where it forms a complex with the B subunits to exert its toxic effects. The disruption occurs when the catalytic A subunit enters the host cell after the B subunit binds to the GM1 gangliosides on the cell membrane. The continuous activation of adenylate cyclase increases intracellular cyclic AMP (cAMP) levels leading to chloride ion and water secretion into the intestinal lumen.

Pathways

The elevation of cAMP levels driven by E. coli heat labile toxin A activates the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel pathway. This process closely relates to the protein Gs alpha which mediates the signal transduction process by activating adenylate cyclase. Another important pathway involves signal crosstalk with the PKC and PKA pathways influencing the regulation of ion transport and fluid secretion in epithelial cells.

E. coli heat labile toxin A links with conditions such as cholera-like diarrhea and traveler's diarrhea. Both of these conditions result from excessive fluid loss due to enhanced chloride ion secretion. The toxin-related pathway involves interactions with proteins such as Gs alpha and CFTR critical components in pathogenesis. Because of the effects on chloride ion transport researchers also study LT-A for its role in understanding cystic fibrosis symptoms and potential treatments.
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Specifications

Form

Liquid

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General info

Function

The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.

Sequence similarities

Belongs to the enterotoxin A family.

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Product protocols

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Target data

The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.
See full target information eltA
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Complementary Products

Primary Antibodies

AB188541

Anti-LT subunit (A + B) antibody

Immunoprecipitation - Anti-LT subunit (A + B) antibody (AB188541)

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