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AB103303

Recombinant E. coli Ecotin protein

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Recombinant E. coli Ecotin protein is a Escherichia coli K-12 Full Length protein, in the 21 to 162 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

eti, b2209, JW2197, eco, Ecotin

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SDS-PAGE - Recombinant E. coli Ecotin protein (AB103303)
  • SDS-PAGE

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SDS-PAGE - Recombinant E. coli Ecotin protein (AB103303)

15% SDS-PAGE analysis of 3μg ab103303.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P23827

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.29% Sodium chloride, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as E. coli serine protease inhibitor.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAESVQPLEKIAPYPQAEKGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAVVR","proteinLength":"Full Length","predictedMolecularWeight":"18.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":162,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P23827","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ecotin also known as ecotine is a potent serine protease inhibitor with a mass of approximately 16 kDa. Researchers identify Ecotin as a broad-spectrum inhibitor found predominantly in Gram-negative bacteria like Escherichia coli. This protein works mechanically by binding to its target serine proteases through a unique double-headed mechanism effectively blocking the enzyme activity.
Biological function summary

This inhibitor controls protease activity important for bacterial survival and pathogenicity. Ecotin functions as an inhibitory protein that interacts closely with several serine proteases preventing them from degrading essential proteins within the bacteria or the host. Ecotin as part of a complex with those proteases contributes to maintaining homeostasis in the microbial environment by limiting proteolytic damage.

Pathways

Ecotin plays an important role in the regulation of the coagulation and inflammatory pathways. It interacts with proteases such as trypsin and chymotrypsin which are vital to these pathways. By inhibiting these proteases Ecotin influences processes that can affect both bacterial survival and host interactions.

Improper regulation of Ecotin might contribute to bacterial infections that involve excessive inflammation. Ecotin has indirect ties with proteins like plasminogen activators which are involved in clot breakdown. Abnormal function of these pathways can lead to conditions such as sepsis and wound healing complications where bacterial pathogens exploit Ecotin's inhibitory capabilities to evade host defenses.
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Specifications

Form

Liquid

Additional notes

ab103303 was purified using conventional chromatography techniques.

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General info

Function

General inhibitor of pancreatic serine proteases : inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases. The strength of inhibition does not appear to be correlated with a particular protease specificity.

Sequence similarities

Belongs to the protease inhibitor I11 (ecotin) family.

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Product protocols

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Target data

General inhibitor of pancreatic serine proteases : inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases. The strength of inhibition does not appear to be correlated with a particular protease specificity.
See full target information eco

Additional targets

Ecotin
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