JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB238375

Recombinant E. coli fimH protein (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant E. coli fimH protein (His tag) is a Escherichia coli (strain K12) Full Length protein, in the 22 to 300 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE. The predicted molecular weight of ab238375 protein is 31.1 kDa.

- Save time and ensure accurate results - use our E. coli Type 1 fimbrin D-mannose specific adhesin (FimH) protein as a control

View Alternative Names

b4320, JW4283, fimH, Type 1 fimbrin D-mannose specific adhesin, Protein FimH

1 Images
SDS-PAGE - Recombinant E. coli fimH protein (His tag) (AB238375)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli fimH protein (His tag) (AB238375)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel of ab238375.

Key facts

Purity

>90% SDS-PAGE

Expression system

Yeast

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P08191

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Ensure the validity of your result using our recombinant E. coli Type 1 fimbrin D-mannose specific adhesin (FimH) protein ab238375 as a positive control in SDS-PAGE.


Check out our protein gel staining guide for SDS-PAGE here
style
left-column

Sequence info

[{"sequence":"FACKTANGTAIPIGGGSANVYVNLAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSSYPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNSDDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYLSGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTANYARTGGQVTAGNVQSIIGVTFVYQ","proteinLength":"Full Length","predictedMolecularWeight":"31.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":300,"aminoAcidStart":22,"nature":"Recombinant","expressionSystem":"Yeast","accessionNumber":"P08191","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The fimH protein also known as FimH adhesin or fimbrial protein serves as an essential adhesion factor in bacteria. With a molecular weight of approximately 29 kDa it is part of the type 1 fimbriae complex and gets highly expressed in Escherichia coli (E. coli). FimH facilitates the attachment of bacteria to host cells by recognizing and binding to mannose residues on the host's surface. This binding is important for the initial colonization and establishment of bacterial infections.
Biological function summary

The fimH protein plays a significant role in E. coli pathogenicity. It acts as part of the type 1 pili enabling the organism to attach firmly to uroepithelial cells and promoting urinary tract infections. As an important component of the fimbrial complex FimH combines with other pilus proteins to create a structure that extends from the bacterial cell surface. This facilitates the evasion of host immune responses and contributes to bacterial virulence.

Pathways

The fimH protein is integral to bacterial adherence and colonization pathways. It interacts with mannose-containing glycoproteins on the host cell surface which is important for the bacterial invasion pathway. FimH association with its pilus subunits like FimC is pivotal for pilus assembly and stability. The protein's role in these pathways highlights its involvement in infections particularly urinary tract infections (UTIs).

The fimH protein holds a strong connection to urinary tract infections and gastrointestinal diseases due to its adhesive capabilities. The protein enables E. coli to attach to and invade host tissues leading to infections. Additionally FimH interacts with the host's uroplakin proteins which are implicated in the bladder epithelium's defense mechanisms. This interaction highlights FimH's contribution to the pathogenic potential of E. coli during bladder infections.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Involved in regulation of length and mediation of adhesion of type 1 fimbriae (but not necessary for the production of fimbriae). Adhesin responsible for the binding to D-mannose. It is laterally positioned at intervals in the structure of the type 1 fimbriae. In order to integrate FimH in the fimbriae FimF and FimG are needed.

Sequence similarities

Belongs to the fimbrial protein family.

style
left-column

Product protocols

style
left-column

Target data

Involved in regulation of length and mediation of adhesion of type 1 fimbriae (but not necessary for the production of fimbriae). Adhesin responsible for the binding to D-mannose. It is laterally positioned at intervals in the structure of the type 1 fimbriae. In order to integrate FimH in the fimbriae FimF and FimG are needed.
See full target information fimH
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB106624

Anti-ZMYM2/RAMP antibody

Western blot - Anti-ZMYM2/RAMP antibody (AB106624)

  • 0
  • 0
Proteins & Peptides

AB313361

Recombinant Human WFDC2 Protein (His tag)

Mass Spectrometry - Recombinant Human WFDC2 Protein (His tag) (AB313361)

  • 0
  • 0
Proteins & Peptides

AB217407

Recombinant human Vitronectin/S-Protein (Animal Free)

  • 0
  • 0
Proteins & Peptides

AB95933

Recombinant human XIAP protein

Functional Studies - Recombinant human XIAP protein (AB95933)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com