Recombinant E. coli Galactosidase alpha protein is a Escherichia coli K-12 Full Length protein, in the 1 to 451 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M M S A P K I T F I G A G S T I F V K N I L G D V F H R E A L K T A H I A L M D I D P T R L E E S H I V V R K L M D S A G A S G K I T C H T Q Q K E A L E D A D F V V V A F Q I G G Y E P C T V T D F E V C K R H G L E Q T I A D T L G P G G I M R A L R T I P H L W Q I C E D M T E V C P D A T M L N Y V N P M A M N T W A M Y A R Y P H I K Q V G L C H S V Q G T A E E L A R D L N I D P A T L R Y R C A G I N H M A F Y L E L E R K T A D G S Y V N L Y P E L L A A Y E A G Q A P K P N I H G N T R C Q N I V R Y E M F K K L G Y F V T E S S E H F A E Y T P W F I K P G R E D L I E R Y K V P L D E Y P K R C V E Q L A N W H K E L E E Y K K A S R I D I K P S R E Y A S T I M N A I W T G E P S V I Y G N V R N D G L I D N L P Q G C C V E V A C L V D A N G I Q P T K V G T L P S H L A A L M Q T N I N V Q T L L T E A I L T E N R D R V Y H A A M M D P H T A A V L G I D E I Y A L V D D L I A A H G D W L P G W L H R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Alternative names
mel-7, b4119, JW4080, Alpha-galactosidase, Melibiase
Recommended products
Recombinant E. coli Galactosidase alpha protein is a Escherichia coli K-12 Full Length protein, in the 1 to 451 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P06720-1
- Animal free
- No
- Species
- Escherichia coli K-12
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M M S A P K I T F I G A G S T I F V K N I L G D V F H R E A L K T A H I A L M D I D P T R L E E S H I V V R K L M D S A G A S G K I T C H T Q Q K E A L E D A D F V V V A F Q I G G Y E P C T V T D F E V C K R H G L E Q T I A D T L G P G G I M R A L R T I P H L W Q I C E D M T E V C P D A T M L N Y V N P M A M N T W A M Y A R Y P H I K Q V G L C H S V Q G T A E E L A R D L N I D P A T L R Y R C A G I N H M A F Y L E L E R K T A D G S Y V N L Y P E L L A A Y E A G Q A P K P N I H G N T R C Q N I V R Y E M F K K L G Y F V T E S S E H F A E Y T P W F I K P G R E D L I E R Y K V P L D E Y P K R C V E Q L A N W H K E L E E Y K K A S R I D I K P S R E Y A S T I M N A I W T G E P S V I Y G N V R N D G L I D N L P Q G C C V E V A C L V D A N G I Q P T K V G T L P S H L A A L M Q T N I N V Q T L L T E A I L T E N R D R V Y H A A M M D P H T A A V L G I D E I Y A L V D D L I A A H G D W L P G W L H R
- Accession
- P06720
- Protein length
- Full Length
- Predicted molecular weight
- 53 kDa
- Amino acids
- 1 to 451
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab126658 is purified using conventional chromatography techniques
General info
- Sequence similarities
Belongs to the glycosyl hydrolase 4 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-galactosidase also known as alpha-D-galactosidase or A-galactosidase is an enzyme that catalyzes the hydrolysis of terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It has a molecular mass of approximately 50000 Daltons. This enzyme is expressed in various tissues including the liver heart and kidneys. It functions by efficiently removing alpha-galactose residues from target molecules a process important for maintaining cellular function.
- Biological function summary
The role of alpha-galactosidase involves the breakdown of complex carbohydrates particularly those containing alpha-galactosidic bonds. It does not operate as part of a complex but rather functions independently to cleave these specific bonds in glycolipids and glycoproteins. This activity is critical for degrading langerin and other gangliosides preventing the accumulation of these molecules in the lysosomes. By continuously performing this function alpha-galactosidase maintains glycosphingolipid homeostasis ensuring efficient cellular metabolism.
- Pathways
The involvement of alpha-galactosidase in metabolic and lysosomal catabolic pathways is significant. One critical pathway is the glycolipid metabolism pathway where it acts alongside related enzymes such as beta-galactosidase. It also participates in the lysosomal degradation pathway working in conjunction with other lysosomal enzymes to prevent substrate buildup. The interaction with enzymes like lysosomal hydrolases ensures the proper breakdown of complex molecules into simpler ones that cells can utilize or excrete.
- Associated diseases and disorders
Defects in the alpha-galactosidase enzyme lead to the lysosomal storage disorder known as Fabry disease. This genetic disorder causes the accumulation of globotriaosylceramide due to deficient alpha-galactosidase activity. Another disorder associated with malfunctioning alpha-galactosidase is cardiac complications due to tissue storage of glycolipids. In both cases the lack of enzyme activity disrupts cellular and tissue functions linking alpha-galactosidase to clinical symptoms seen in these disorders.
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1 product image
SDS-PAGE - Recombinant E. coli Galactosidase alpha protein (ab126658)
15% SDS-PAGE showing ab126658 (3µg).
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