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AB119740

Recombinant E. coli GFP protein (His tag)

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(4 Publications)

Recombinant E. coli GFP protein (His tag) is a Aequorea victoria Full Length protein, in the 1 to 238 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, WB.

View Alternative Names

Green fluorescent protein, GFP

1 Images
SDS-PAGE - Recombinant E. coli GFP protein (His tag) (AB119740)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant E. coli GFP protein (His tag) (AB119740)

The purity of ab119740 was determined to be >80% by densitometry.

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

P42212

Animal free

No

Carrier free

No

Species

Aequorea victoria

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.71% Sodium phosphate, 0.005% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

AB171581

GFP ELISA Kit

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Sandwich ELISA - GFP ELISA Kit (AB171581)

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Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

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Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"39 kDa","actualMolecularWeight":null,"aminoAcidEnd":238,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P42212","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

The purity was determined to be 80% by densitometry

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General info

Function

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Sequence similarities

Belongs to the GFP family.

Post-translational modifications

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

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Product protocols

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Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials 288:121671 PubMed35953331

2022

Debulking different Corona (SARS-CoV-2 delta, omicron, OC43) and Influenza (H1N1, H3N2) virus strains by plant viral trap proteins in chewing gums to decrease infection and transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Henry Daniell,Smruti K Nair,Hancheng Guan,Yuwei Guo,Rachel J Kulchar,Marcelo D T Torres,Md Shahed-Al-Mahmud,Geetanjali Wakade,Yo-Min Liu,Andrew D Marques,Jevon Graham-Wooten,Wan Zhou,Ping Wang,Sudheer K Molugu,William R de Araujo,Cesar de la Fuente-Nunez,Che Ma,William R Short,Pablo Tebas,Kenneth B Margulies,Frederic D Bushman,Francis K Mante,Robert P Ricciardi,Ronald G Collman,Mark S Wolff

MicrobiologyOpen 10:e1239 PubMed34713604

2021

Characterization of endogenous promoters of GapC1 and GS for recombinant protein expression in Phaeodactylum tricornutum.

Applications

Unspecified application

Species

Unspecified reactive species

Erdenedolgor Erdene-Ochir,Bok-Kyu Shin,Md Nazmul Huda,Eun Ha Lee,Dae-Geun Song,Choonkyun Jung,Cheol-Ho Pan

BMC plant biology 21:208 PubMed33952221

2021

Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus.

Applications

Unspecified application

Species

Unspecified reactive species

Wenshuang Xie,Dee Marie Marty,Junhuan Xu,Nitika Khatri,Kristen Willie,Wanderson Bucker Moraes,Lucy R Stewart

Scientific reports 9:9941 PubMed31289300

2019

Identification and characterisation of the novel endogenous promoter HASP1 and its signal peptide from Phaeodactylum tricornutum.

Applications

Unspecified application

Species

Unspecified reactive species

Erdenedolgor Erdene-Ochir,Bok-Kyu Shin,Byeori Kwon,Choonkyun Jung,Cheol-Ho Pan
View all publications
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Product promise

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