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Recombinant E. coli GFP protein (His tag) is a Aequorea victoria Full Length protein, in the 1 to 238 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, WB.
Alternative names=Green fluorescent protein, GFP
- Purity
>80% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, WB
- Biologically active
No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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Recombinant E. coli GFP protein (His tag) is a Aequorea victoria Full Length protein, in the 1 to 238 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, WB.
Alternative names=Green fluorescent protein, GFP
Key facts
- Purity
>80% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, WB
- Biologically active
No
- Predicted molecular weight
39 kDa
- Amino acids
1 to 238
- Accession
- P42212-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Aequorea victoria
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.71% Sodium phosphate, 0.005% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Specifications
- Form
Liquid
- Additional notes
The purity was determined to be 80% by densitometry
General info
- Function
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
- Sequence similarities
Belongs to the GFP family.
- Post-translational modifications
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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