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AB107143

Recombinant E. coli glsA1 protein

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Recombinant E. coli glsA1 protein is a Escherichia coli K-12 Full Length protein, in the 1 to 310 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ybaS, b0485, JW0474, glsA1, Glutaminase 1

1 Images
SDS-PAGE - Recombinant E. coli glsA1 protein (AB107143)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli glsA1 protein (AB107143)

ab107143 at 3 μg analysed by 15% SDS PAGE.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P77454

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVAIVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVIALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQSEQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTIELATLGATLAAGGVNPLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGIAAFSPPLDEDGNSVRGQKMVASVAKQLGYNVFKG","proteinLength":"Full Length","predictedMolecularWeight":"35 kDa","actualMolecularWeight":null,"aminoAcidEnd":310,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P77454","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target glsA1 also known as Glutaminase 1 is an enzyme involved in the hydrolysis of glutamine to glutamate and ammonia playing an important role in nitrogen metabolism. It has a molecular mass of approximately 65 kDa. This enzyme is expressed in various tissues including the liver kidneys and brain where it assists in maintaining glutamate levels required for numerous cellular functions.
Biological function summary

GlsA1 serves an essential role in energy production and neurotransmitter cycling. As an important contributor to the glutaminase complex it ensures the conversion of glutamine into glutamate which neurons in the central nervous system use for signaling. This conversion process supports neurotransmitter balance and overall neural function impacting brain health and activity.

Pathways

The enzyme glsA1 plays a significant position in the glutaminolysis and tricarboxylic acid (TCA) cycle pathways. It assists in integrating amino acid metabolism with energy production. Through its interaction with other proteins such as GLUD1 glsA1 influences the regulation of oxidative phosphorylation. These pathways are critical for cellular energy homeostasis demonstrating the enzyme’s role in metabolic adaptability.

GlsA1 displays a link to certain cancers including glioblastoma where increased expression has been observed suggesting an association with tumor growth and survival. Moreover glsA1 is related to hepatic encephalopathy displaying connections with GLUD1 in metabolic disturbances. These associations indicate the relevance of glsA1 in understanding metabolic reprogramming in disease contexts.
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Specifications

Form

Liquid

Additional notes

Purified by using conventional chromatography techniques

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General info

Sequence similarities

Belongs to the glutaminase family.

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Product protocols

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Target data

See full target information glsA1
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