Recombinant E. coli Glucose 6 Phosphate Dehydrogenase protein is a Escherichia coli K-12 Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A V T Q T A Q A C D L V I F G A K G D L A R R K L L P S L Y Q L E K A G Q L N P D T R I I G V G R A D W D K A A Y T K V V R E A L E T F M K E T I D E G L W D T L S A R L D F C N L D V N D T A A F S R L G A M L D Q K N R I T I N Y F A M P P S T F G A I C K G L G E A K L N A K P A R V V M E K P L G T S L A T S Q E I N D Q V G E Y F E E C Q V Y R I D H Y L G K E T V L N L L A L R F A N S L F V N N W D N R T I D H V E I T V A E E V G I E G R W G Y F D K A G Q M R D M I Q N H L L Q I L C M I A M S P P S D L S A D S I R D E K V K V L K S L R R I D R S N V R E K T V R G Q Y T A G F A Q G K K V P G Y L E E E G A N K S S N T E T F V A I R V D I D N W R W A G V P F Y L R T G K R L P T K C S E V V V Y F K T P E L N L F K E S W Q D L P Q N K L T I R L Q P D E G V D I Q V L N K V P G L D H K H N L Q I T K L D L S Y S E T F N Q T H L A D A Y E R L L L E T M R G I Q A L F V R R D E V E E A W K W V D S I T E A W A M D N D A P K P Y Q A G T W G P V A S V A M I T R D G R S W N E F E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone. Probable source of extracellular death factor (EDF, sequence Asn-Asn-Trp-Asn-Asn, NNWNN) following processing and amidation. This pentapeptide stimulates cell death mediated by MazF (PubMed:17962566). Artificial peptides with altered sequence show that NNGNN, GNWNG and NWN no longer stimulate MazF's endoribonuclease activity; other peptides (NNGN, GNWMM, NNWNG, NNNWNNN) retain MazF-stimulating activity. NNWNN, NNGN, GNWMM and NNWNG prevent cognate antitoxin MazE from inhibiting MazF; although NNNWNNN stimulates MazF it does not do so in the presence of MazE. EDF also stimulates ChpB's endoribonuclease activity in vitro; in this case NWN partially stimulates ChpB, whereas NNGNN, GNWNN, NNWNG, GNWNG and NNNWNNN do not. Only the wild-type EDF peptide prevents cognate antitoxin ChpS from inhibiting ChpB (PubMed:21419338).
Alternative names
b1852, JW1841, Glucose-6-phosphate 1-dehydrogenase, G6PD
Recommended products
Recombinant E. coli Glucose 6 Phosphate Dehydrogenase protein is a Escherichia coli K-12 Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C. Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
- Accession
- P0AC53-1
- Animal free
- No
- Species
- Escherichia coli K-12
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10% Glycerol (glycerin, glycerine), 0.975% MES, 0.0584% EDTA, 0.0077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
- M A V T Q T A Q A C D L V I F G A K G D L A R R K L L P S L Y Q L E K A G Q L N P D T R I I G V G R A D W D K A A Y T K V V R E A L E T F M K E T I D E G L W D T L S A R L D F C N L D V N D T A A F S R L G A M L D Q K N R I T I N Y F A M P P S T F G A I C K G L G E A K L N A K P A R V V M E K P L G T S L A T S Q E I N D Q V G E Y F E E C Q V Y R I D H Y L G K E T V L N L L A L R F A N S L F V N N W D N R T I D H V E I T V A E E V G I E G R W G Y F D K A G Q M R D M I Q N H L L Q I L C M I A M S P P S D L S A D S I R D E K V K V L K S L R R I D R S N V R E K T V R G Q Y T A G F A Q G K K V P G Y L E E E G A N K S S N T E T F V A I R V D I D N W R W A G V P F Y L R T G K R L P T K C S E V V V Y F K T P E L N L F K E S W Q D L P Q N K L T I R L Q P D E G V D I Q V L N K V P G L D H K H N L Q I T K L D L S Y S E T F N Q T H L A D A Y E R L L L E T M R G I Q A L F V R R D E V E E A W K W V D S I T E A W A M D N D A P K P Y Q A G T W G P V A S V A M I T R D G R S W N E F E
- Accession
- P0AC53
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Purified by conventional chromatography techniques.
General info
- Function
Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.
- Sequence similarities
Belongs to the glucose-6-phosphate dehydrogenase family.
- Post-translational modifications
Probably processed by the ClpPX protease to generate the extracellular death factor (EDF). It is thought that processing produces Asn-Asn-Trp-Asp-Asn which is amidated to generate Asn-Asn-Trp-Asn-Asn (Probable).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glucose 6 Phosphate Dehydrogenase also known as G6PD or G6P dehydrogenase plays an important role in the pentose phosphate pathway catalyzing the conversion of glucose 6-phosphate to 6-phosphoglucono-δ-lactone while producing NADPH from NADP+. G6PD with a molecular mass of approximately 59 kDa is expressed in various tissues with high levels found in red blood cells liver and adrenal glands. This enzyme is vital for protecting cells from oxidative damage by supplying reductive capacity.
- Biological function summary
Glucose 6-phosphate dehydrogenase is essential for maintaining cellular redox balance especially in cells lacking mitochondria like red blood cells. It functions as part of a monomer which can dimerize depending on the cellular needs and conditions. The activity of G6PD directly affects the production of NADPH which is necessary for the biosynthesis of nucleic acids and lipids and for maintaining reduced glutathione levels therefore sustaining the antioxidant capacity of the cell.
- Pathways
Glucose 6-phosphate dehydrogenase is a pivotal enzyme in the oxidative phase of the pentose phosphate pathway which provides ribose 5-phosphate for nucleotide synthesis and NADPH for reductive biosynthetic reactions. G6PD connects with other enzymes in the pathway such as 6-phosphogluconate dehydrogenase contributing to the regulation of cellular metabolic needs. Its function is tightly linked to glucose metabolism and indirectly influences glycolytic processes.
- Associated diseases and disorders
Alterations in glucose 6-phosphate dehydrogenase activity lead to conditions such as G6PD deficiency which can cause hemolytic anemia when individuals are exposed to certain drugs infections or foods. This enzyme's deficiency is one of the most common enzymatic disorders in humans and is triggered by genetic mutations affecting G6PD function. Moreover G6PD has implications in cancer biology as its activity influences the proliferation and survival of cancer cells alongside other proteins like TP53 and NF-κB which are involved in cellular stress responses.
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SDS-PAGE - Recombinant E. coli Glucose 6 Phosphate Dehydrogenase protein (ab87414)
ab87414 on 15% SDS-PAGE (4μg)
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