JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB235854

Recombinant E. coli Glycerol 3 Phosphate Dehydrogenase protein (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant E. coli Glycerol 3 Phosphate Dehydrogenase protein (His tag) is a Escherichia coli K-12 Full Length protein, in the 1 to 501 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

glyD, b3426, JW3389, glpD, Aerobic glycerol-3-phosphate dehydrogenase

1 Images
SDS-PAGE - Recombinant E. coli Glycerol 3 Phosphate Dehydrogenase protein (His tag) (AB235854)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli Glycerol 3 Phosphate Dehydrogenase protein (His tag) (AB235854)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel using ab235854.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P13035

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSASSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIRIGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVVRKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGMHLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLNVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDYTLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIEGDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAELKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSLAS","proteinLength":"Full Length","predictedMolecularWeight":"60.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":501,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P13035","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycerol 3-Phosphate Dehydrogenase (G3PDH) also known as G3P dehydrogenase and glycerol-3-phosphate dehydrogenase plays a mechanical role in the reversible redox conversion of dihydroxyacetone phosphate (DHAP) to glycerol 3-phosphate. The enzyme typically weighs around 40-50 kDa although the exact mass depends on isoform. G3PDH is expressed in various tissues with significant presence in the brain liver and skeletal muscle reflecting its involvement in energy metabolism. The mitochondrial and cytosolic forms add to its versatility in cellular compartments.
Biological function summary

This enzyme is critical for lipid and carbohydrate metabolism engaging extensively in energy homeostasis. G3PDH exists as part of a complex to facilitate its role in mitochondrial electron transport. It acts as a shuttle transferring electrons from cytosolic NADH into the mitochondria. This electron transport is essential for synthesizing ATP thereby linking metabolic pathways within cells.

Pathways

Glycerol-3-phosphate dehydrogenase participates actively in the glycerol phosphate shuttle and lipid biosynthesis pathways. It pairs functionally with its partner the mitochondrial glycerol 3-phosphate dehydrogenase (mG3PDH) to support efficient energy production and lipid storage. Additionally this enzyme relates closely to proteins like NADH dehydrogenase playing a role in electron transport chains in mitochondria critical for bioenergetics.

G3PDH has connections to metabolic conditions such as obesity and diabetes. Impaired function of this enzyme can lead to disruptions in metabolic pathways which can contribute to these diseases. It also holds significance in cancer metabolism with altered expression of G3PDH seen in various tumors. The enzyme's relation to proteins like mG3PDH highlights its role in metabolic syndrome and associated pathologies.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses molecular oxygen or nitrate as electron acceptor.

Sequence similarities

Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

style
left-column

Product protocols

style
left-column

Target data

Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses molecular oxygen or nitrate as electron acceptor.
See full target information glpD
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB109004

Anti-PAP antibody [EPR4067]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PAP antibody [EPR4067] (AB109004)

  • 4
  • 1
Proteins & Peptides

AB283941

Recombinant Human Sirtuin 2/SIRT2 Protein

Mass Spectrometry - Recombinant Human Sirtuin 2/SIRT2 Protein (AB283941)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com