Recombinant E. coli Maltose Binding Protein is a Escherichia coli K-12 Full Length protein, in the 27 to 396 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M K I E E G K L V I W I N G D K G Y N G L A E V G K K F E K D T G I K V T V E H P D K L E E K F P Q V A A T G D G P D I I F W A H D R F G G Y A Q S G L L A E I T P D K A F Q D K L Y P F T W D A V R Y N G K L I A Y P I A V E A L S L I Y N K D L L P N P P K T W E E I P A L D K E L K A K G K S A L M F N L Q E P Y F T W P L I A A D G G Y A F K Y E N G K Y D I K D V G V D N A G A K A G L T F L V D L I K N K H M N A D T D Y S I A E A A F N K G E T A M T I N G P W A W S N I D T S K V N Y G V T V L P T F K G Q P S K P F V G V L S A G I N A A S P N K E L A K E F L E N Y L L T D E G L E A V N K D K P L G A V A L K S Y E E E L A K D P R I A A T M E N A Q K G E I M P N I P Q M S A F W Y A V R T A V I N A A S G R Q T V D E A L K D A Q T R I T K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose.
Additional Targets
Maltose Binding Protein
Alternative names
b4034, JW3994, malE, Maltose/maltodextrin-binding periplasmic protein, MMBP, Maltodextrin-binding protein, Maltose-binding protein, MBP
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Recombinant E. coli Maltose Binding Protein is a Escherichia coli K-12 Full Length protein, in the 27 to 396 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P0AEX9-1
- Animal free
- No
- Species
- Escherichia coli K-12
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M K I E E G K L V I W I N G D K G Y N G L A E V G K K F E K D T G I K V T V E H P D K L E E K F P Q V A A T G D G P D I I F W A H D R F G G Y A Q S G L L A E I T P D K A F Q D K L Y P F T W D A V R Y N G K L I A Y P I A V E A L S L I Y N K D L L P N P P K T W E E I P A L D K E L K A K G K S A L M F N L Q E P Y F T W P L I A A D G G Y A F K Y E N G K Y D I K D V G V D N A G A K A G L T F L V D L I K N K H M N A D T D Y S I A E A A F N K G E T A M T I N G P W A W S N I D T S K V N Y G V T V L P T F K G Q P S K P F V G V L S A G I N A A S P N K E L A K E F L E N Y L L T D E G L E A V N K D K P L G A V A L K S Y E E E L A K D P R I A A T M E N A Q K G E I M P N I P Q M S A F W Y A V R T A V I N A A S G R Q T V D E A L K D A Q T R I T K
- Accession
- P0AEX9
- Protein length
- Full Length
- Predicted molecular weight
- 40.8 kDa
- Amino acids
- 27 to 396
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab219252 was purified by conventional chromatography techniques.
General info
- Function
Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose.
- Sequence similarities
Belongs to the bacterial solute-binding protein 1 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Maltose Binding Protein often referred to as MBP is a periplasmic protein that plays an important role in the transportation of maltose and maltodextrins in E. coli. It binds to maltose with high specificity facilitating its uptake into the bacterial cell. The MBP protein size is approximately 42 kDa which allows it to effectively interact with its ligands. Scientists often use MBP as a fusion partner in protein purification processes due to its solubility and ability to enhance the expression of recombinant proteins.
- Biological function summary
Within the cell it functions by ensuring the effective transport of sugars across the bacterial membrane. Since MBP is not typically a part of a larger complex it acts more as an individual unit facilitating the binding of its target sugars. This binding and transport mechanism is vital for the bacterial carbohydrate metabolism supporting the overall energy balance within the microorganism.
- Pathways
This protein is integral to the maltose/maltodextrin transport system which is a part of the larger phosphotransferase system. Its role in these pathways is closely associated with other proteins such as the Maltose ABC transporter proteins which form a transport mechanism across the bacterial inner membrane. Additionally MBP has a functional relationship with the enzymes in the glycolysis pathway as the transported sugars eventually feed into this energy-producing route.
- Associated diseases and disorders
Maltose binding protein itself is not a direct causative factor. However understanding MBP can assist in researching bacterial infections caused by pathogenic strains like E. coli where maltose metabolism is important for survival and virulence. Moreover disruptions in sugar transport pathways involving proteins like MBP can contribute to metabolic imbalances. Although MBP is not directly implicated in human diseases analyzing its function can aid in developing therapeutic approaches targeting sugar transport systems in harmful bacteria.
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SDS-PAGE - Recombinant E. coli Maltose Binding Protein (ab219252)
15% SDS-PAGE analysis of 3 μg ab219252.
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