Recombinant E. coli mdh protein (His tag N-Terminus)

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Biological function summary

Malate dehydrogenase is essential in energy production and cellular respiration. The enzyme operates in both the mitochondria and cytosol indicating its ability to shuttle metabolic intermediates between different cellular compartments. MDH does not typically form part of a multi-subunit complex but can interact closely with other dehydrogenases to facilitate metabolic flux. By efficiently interchanging between malate and oxaloacetate MDH maintains the balance of redox states and supports ATP generation.

Pathways

Malate dehydrogenase plays a role in the citric acid cycle and the malate-aspartate shuttle which are central metabolic pathways. In the citric acid cycle it influences the homeostasis of NAD⁺ and NADH which is important for effective energy yield. The malate-aspartate shuttle where MDH works alongside aspartate aminotransferase aids in transferring reducing equivalents across the mitochondrial membrane. This cooperation assists in maintaining the efficiency of oxidative phosphorylation and ATP synthesis.