Recombinant E. coli Multi Tag protein

• WB

Lab

Western blot - Recombinant E. coli Multi Tag protein (AB36791)

Lane 1:

HA-HRP

Lanes 1 - 11:

Western blot - Recombinant E. coli Multi Tag protein (ab36791) at 0.5 µL

Lane 2:

Myc-HRP

Lane 3:

Glu-Glu

Lane 4:

GST

Lane 5:

His

Lane 6:

HSV

Lane 7:

T7

Lane 8:

V5

Lane 9:

VSV

Lane 10:

S-Tag

Lane 11:

FLAG-HRP

false

• WB

Lab

Western blot - Recombinant E. coli Multi Tag protein (AB36791)

Blocking buffer : 2% BSA Gel type : MOPS

All lanes:

Western blot - HRP Anti-Myc tag antibody [9E10] (<a href='/en-us/products/primary-antibodies/hrp-myc-tag-antibody-9e10-ab62928'>ab62928</a>) at 1/10000 dilution

All lanes:

Western blot - Recombinant E. coli Multi Tag protein (ab36791) at 0.5 µg

Predicted band size: 49 kDa

Observed band size: 45 kDa

false

Exposure time: 4min

• WB

Lab

Western blot - Recombinant E. coli Multi Tag protein (AB36791)

Anti-V5 tag antibody [SV5-P-K] staining at 1/1000 dilution, shown in black. In Western blot, ab206566 was shown to bind specifically to V5 tag. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 3 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) at 1/50000 dilution.

All lanes:

Western blot - Anti-V5 tag antibody [SV5-P-K] (<a href='/en-us/products/primary-antibodies/v5-tag-antibody-sv5-p-k-ab206566'>ab206566</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant E. coli Multi Tag protein (ab36791) at 0.2 µg

Lane 2:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab273530'>ab273530</a>) at 0.2 µg

Observed band size: 45 kDa

false

Exposure time: 3min