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Recombinant E. coli Multi Tag protein is a protein, expressed in Escherichia coli and suitable for WB.
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
WB
- Biologically active
No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes Use 0.5 - 2 μl for western blot. |
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Recombinant E. coli Multi Tag protein is a protein, expressed in Escherichia coli and suitable for WB.
Key facts
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
WB
- Biologically active
No
- Predicted molecular weight
45 kDa
- Animal free
No
- Nature
Synthetic
- Concentration
- Storage buffer
pH: 6.8
Constituents: 10% Glycerol (glycerin, glycerine), 2% Sodium lauryl sulfate, 0.76% Tris, 0.01% 3,3-bis(3,5-dibromo-4-hydroxyphenyl)-3H-2,1λ⁶-benzoxathiole-1,1-dione
Specifications
- Form
Liquid
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This protein lysate contains the commonly used epitope tags: HA, c-myc, Glu-Glu, GST, 6-His, HSV, T7, V5, VSV, E-tag, S-tag, FLAG (TM). These proteins were fused in the following order, GST, T7, HSV, c-myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag and HA, 6xHis.
This product is a bacterial lysate provided in1 x reduced SDS-PAGE loading buffer. It is effective as a positive control for epitope tag antibodies in immunoblotting.
For Western blotting, the suggested use of this reagent is 0.5 - 2.0 μl. It is recommended that the reagent be titrated for optimal performance for each application.
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