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Recombinant E. coli Multi Tag protein is a protein, expressed in Escherichia coli and suitable for WB.

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Images

Western blot - Recombinant E. coli Multi Tag protein (AB36791), expandable thumbnail
  • Western blot - Recombinant E. coli Multi Tag protein (AB36791), expandable thumbnail
  • Western blot - Recombinant E. coli Multi Tag protein (AB36791), expandable thumbnail

Publications

Key facts

Expression system
Escherichia coli
Tags
HA tag Myc tag Glu-Glu tag GST tag 6x His tag HSV tag T7 tag V5 tag VSV-G tag E-tag tag S-tag tag DDDDK tag
Applications
WB
Biologically active
No

Reactivity data

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

Use 0.5 - 2 μl for western blot.

Target data

Recommended products

Recombinant E. coli Multi Tag protein is a protein, expressed in Escherichia coli and suitable for WB.

Key facts

Expression system
Escherichia coli
Applications
WB
Animal free
No
Concentration
Loading...
Storage buffer

pH: 6.8
Constituents: 10% Glycerol (glycerin, glycerine), 2% Sodium lauryl sulfate, 0.76% Tris, 0.01% 3,3-bis(3,5-dibromo-4-hydroxyphenyl)-3H-2,1λ⁶-benzoxathiole-1,1-dione

Sequence info

Amino acid sequence

Accession
Predicted molecular weight
45 kDa
Nature
Synthetic
Tags
HA tag, Myc tag, Glu-Glu tag, GST tag, 6x His tag, HSV tag, T7 tag, V5 tag, VSV-G tag, E-tag tag, S-tag tag, DDDDK tag

Specifications

Form
Liquid

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This protein lysate contains the commonly used epitope tags: HA, c-myc, Glu-Glu, GST, 6-His, HSV, T7, V5, VSV, E-tag, S-tag, FLAG (TM). These proteins were fused in the following order, GST, T7, HSV, c-myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag and HA, 6xHis.

This product is a bacterial lysate provided in1 x reduced SDS-PAGE loading buffer. It is effective as a positive control for epitope tag antibodies in immunoblotting.

For Western blotting, the suggested use of this reagent is 0.5 - 2.0 μl. It is recommended that the reagent be titrated for optimal performance for each application.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Recombinant E. coli Multi Tag protein (ab36791), expandable thumbnail

    Western blot - Recombinant E. coli Multi Tag protein (ab36791)

    Lane 1: HA-HRP

    Lanes 1 - 11: Western blot - Recombinant E. coli Multi Tag protein (ab36791) at 0.5 µL

    Lane 2: Myc-HRP

    Lane 3: Glu-Glu

    Lane 4: GST

    Lane 5: His

    Lane 6: HSV

    Lane 7: T7

    Lane 8: V5

    Lane 9: VSV

    Lane 10: S-Tag

    Lane 11: FLAG-HRP

  • Western blot - Recombinant E. coli Multi Tag protein (ab36791), expandable thumbnail

    Western blot - Recombinant E. coli Multi Tag protein (ab36791)

    Blocking buffer: 2% BSA
    Gel type: MOPS

    All lanes: Western blot - HRP Anti-Myc tag antibody [9E10] (HRP Anti-Myc tag antibody [9E10] ab62928) at 1/10000 dilution

    All lanes: Western blot - Recombinant E. coli Multi Tag protein (ab36791) at 0.5 µg

    Predicted band size: 49 kDa

    Observed band size: 45 kDa

    Exposure time: 4min

  • Western blot - Recombinant E. coli Multi Tag protein (ab36791), expandable thumbnail

    Western blot - Recombinant E. coli Multi Tag protein (ab36791)

    Anti-V5 tag antibody [SV5-P-K] staining at 1/1000 dilution, shown in black. In Western blot, Anti-V5 tag antibody [SV5-P-K] ab206566 was shown to bind specifically to V5 tag. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent Anti-PKC delta (phospho S299) antibody [EPNCI119] ab133456) and imaged with 3 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) at 1/50000 dilution.

    All lanes: Western blot - Anti-V5 tag antibody [SV5-P-K] (Anti-V5 tag antibody [SV5-P-K] ab206566) at 1/1000 dilution

    Lane 1: Western blot - Recombinant E. coli Multi Tag protein (ab36791) at 0.2 µg

    Lane 2: Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) ab273530) at 0.2 µg

    Performed under reducing conditions.

    Observed band size: 45 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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