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AB225594

Recombinant E. coli Murein hydrolase activator NlpD protein (His tag)

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(1 Publication)

Recombinant E. coli Murein hydrolase activator NlpD protein (His tag) is a Escherichia coli K-12 Full Length protein, in the 26 to 379 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

b2742, JW2712, nlpD, Murein hydrolase activator NlpD

1 Images
SDS-PAGE - Recombinant E. coli Murein hydrolase activator NlpD protein (His tag) (AB225594)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli Murein hydrolase activator NlpD protein (His tag) (AB225594)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225594 with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P0ADA3

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"CSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR","proteinLength":"Full Length","predictedMolecularWeight":"53.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":379,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P0ADA3","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Murein hydrolase activator NlpD also known as lipoprotein NlpD facilitates the regulation of peptidoglycan hydrolysis in bacterial cell walls. The protein assists in activating murein hydrolase enzymes which are essential for cell wall metabolism particularly during cell division. NlpD is a periplasmic protein with an approximate mass of 45 kDa and it is expressed in Gram-negative bacteria residing within the inner membrane.
Biological function summary

The murein hydrolase activator plays a significant role in bacterial cell growth and division. NlpD is involved in the coordination of septum formation and cell wall remodeling by its association with specific peptidoglycan hydrolases. It does not form a complex however its activity is closely regulated by its interaction with other cell division proteins facilitating precise control over cell wall degradation and renewal processes.

Pathways

NlpD integrates into the broader framework of cell envelope biosynthesis and cell cycle regulation. The protein participates in peptidoglycan turnover and is connected with the division septum positioning pathway. It interacts with proteins like FtsZ and AmiC helping organize the activity of enzymatic complexes necessary for cytokinesis and cell shape maintenance.

Perturbations involving NlpD can contribute to pathogenicity in bacteria enhancing their virulence. Changes in the activity or expression of NlpD may play a role in conditions like antibiotic resistance and certain infections. The protein's dysregulation can affect the bacteria’s ability to adapt its cell wall under antibiotic pressure linking it functionally to other resistance factors and mechanisms within the bacterial cell.
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Specifications

Form

Liquid

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General info

Function

Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.

Sequence similarities

Belongs to the E.coli NlpD/Haemophilus LppB family.

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Product protocols

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Target data

Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.
See full target information nlpD

Additional targets

Murein hydrolase activator NlpD
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genome medicine 17:65 PubMed40457459

2025

Associations between HLA-II variation and antibody specificity are predicted by antigen properties.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel Innocenti,Sergio Andreu-Sánchez,Nicolai V Hörstke,Hesham Elabd,Iros Barozzi,Andre Franke,Máté Manczinger,Thomas Vogl
View all publications
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