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AB78932

Recombinant E. coli nusA protein (Tag Free)

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Recombinant E. coli nusA protein (Tag Free) is a Escherichia coli K-12 Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

b3169, JW3138, nusA, Transcription termination/antitermination protein NusA, N utilization substance protein A, Transcription termination/antitermination L factor

1 Images
SDS-PAGE - Recombinant E. coli nusA protein (Tag Free) (AB78932)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant E. coli nusA protein (Tag Free) (AB78932)

ab78932 on 15% SDS-PAGE (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P0AFF6

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P0AFF6","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NusA protein sometimes referred to simply as nusA is a highly conserved transcription factor in bacteria. It plays an essential role in the regulation of transcriptional elongation termination and anti-termination. NusA has a mass of approximately 55 kDa and can be identified in the cytoplasm of prokaryotic cells where it interacts directly with RNA polymerase. It contains distinct domains including an N-terminal S1 domain and several K-homology (KH) domains which facilitate its multiple interactions throughout the transcription process.
Biological function summary

NusA plays an important role in modulating RNA polymerase activity by temporally pausing transcription which allows for proper folding and processing of the RNA transcript. It functions as part of a larger transcription complex working with other proteins such as NusB NusG and ribosomal proteins. NusA is essential for promoting termination at intrinsic terminators and participates in antitermination mechanisms during bacteriophage lambda N gene transcription. This dual role makes it an important regulator of gene expression and response to environmental changes.

Pathways

NusA is integral to the bacterial transcription regulation and gene expression pathways. It primarily functions in the elongation and termination phases of transcription which links NusA to the regulation of the RNA synthesis pathway. Interaction with NusG can influence the action of RNA polymerase by causing a pause during transcription which affects the overall transcription efficiency and fidelity. NusA's involvement in the intricate regulation of transcription indicates its importance in adaptive cellular responses.

Disruptions in nusA-related pathways can have implications for antibiotic resistance development as it affects transcriptional regulation mechanisms that bacteria may exploit under stress conditions. Additionally studies suggest a link between NusA and the persistence phenotypes in bacterial infections connecting it to the management of chronic bacterial diseases. NusA's interaction with ribosomal proteins like S10 may influence translational stability and efficiency which might further affect bacterial virulence and persistence under hostile conditions.
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Specifications

Form

Liquid

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General info

Function

Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNA polymerase (RNAP). It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis (PubMed : 32871103). Participates in both transcription termination and antitermination. Involved in a variety of cellular termination and antitermination processes, such as Rho-dependent transcriptional termination and intrinsic termination (PubMed : 31020314). Domain AR2 interacts with a large number of other proteins and may serve as a platform to recruit these factors for transcriptional regulation (PubMed : 31127279). Involved in phage lambda N-mediated transcriptional antitermination. Also important for coordinating the cellular responses to DNA damage by coupling the processes of nucleotide excision repair and translesion synthesis to transcription.

Sequence similarities

Belongs to the NusA family.

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Product protocols

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Target data

Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNA polymerase (RNAP). It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis (PubMed : 32871103). Participates in both transcription termination and antitermination. Involved in a variety of cellular termination and antitermination processes, such as Rho-dependent transcriptional termination and intrinsic termination (PubMed : 31020314). Domain AR2 interacts with a large number of other proteins and may serve as a platform to recruit these factors for transcriptional regulation (PubMed : 31127279). Involved in phage lambda N-mediated transcriptional antitermination. Also important for coordinating the cellular responses to DNA damage by coupling the processes of nucleotide excision repair and translesion synthesis to transcription.
See full target information nusA
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