Recombinant E. coli Outer membrane protein C (Tagged) is a Escherichia coli K-12 Full Length protein, in the 22 to 367 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
A E V Y N K D G N K L D L Y G K V D G L H Y F S D N K D V D G D Q T Y M R L G F K G E T Q V T D Q L T G Y G Q W E Y Q I Q G N S A E N E N N S W T R V A F A G L K F Q D V G S F D Y G R N Y G V V Y D V T S W T D V L P E F G G D T Y G S D N F M Q Q R G N G F A T Y R N T D F F G L V D G L N F A V Q Y Q G K N G N P S G E G F T S G V T N N G R D A L R Q N G D G V G G S I T Y D Y E G F G I G G A I S S S K R T D A Q N T A A Y I G N G D R A E T Y T G G L K Y D A N N I Y L A A Q Y T Q T Y N A T R V G S L G W A N K A Q N F E A V A Q Y Q F D F G L R P S L A Y L Q S K G K N L G R G Y D D E D I L K Y V D V G A T Y Y F N K N M S T Y V D Y K I N L L D D N Q F T R D A G I N T D N I V A L G L V Y Q F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Forms pores that allow passive diffusion of small molecules across the outer membrane.(Microbial infection) Supports colicin E5 entry in the absence of its major receptor OmpF.(Microbial infection) A mixed OmpC-OmpF heterotrimer is the outer membrane receptor for toxin CdiA-EC536; polymorphisms in extracellular loops 4 and 5 of OmpC confer susceptibility to CdiA-EC536-mediated toxicity.
Alternative names
Outer membrane porin C, Outer membrane protein 1B, Outer membrane protein C, Porin OmpC, meoA, JW2203, b2215, ompC, par
Recommended products
Recombinant E. coli Outer membrane protein C (Tagged) is a Escherichia coli K-12 Full Length protein, in the 22 to 367 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- P06996-1
- Animal free
No
- Species
Escherichia coli K-12
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
A E V Y N K D G N K L D L Y G K V D G L H Y F S D N K D V D G D Q T Y M R L G F K G E T Q V T D Q L T G Y G Q W E Y Q I Q G N S A E N E N N S W T R V A F A G L K F Q D V G S F D Y G R N Y G V V Y D V T S W T D V L P E F G G D T Y G S D N F M Q Q R G N G F A T Y R N T D F F G L V D G L N F A V Q Y Q G K N G N P S G E G F T S G V T N N G R D A L R Q N G D G V G G S I T Y D Y E G F G I G G A I S S S K R T D A Q N T A A Y I G N G D R A E T Y T G G L K Y D A N N I Y L A A Q Y T Q T Y N A T R V G S L G W A N K A Q N F E A V A Q Y Q F D F G L R P S L A Y L Q S K G K N L G R G Y D D E D I L K Y V D V G A T Y Y F N K N M S T Y V D Y K I N L L D D N Q F T R D A G I N T D N I V A L G L V Y Q F
- Accession
- Protein length
Full Length
- Predicted molecular weight
54.3 kDa
- Amino acids
22 to 367
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Forms pores that allow passive diffusion of small molecules across the outer membrane.
- Sequence similarities
Belongs to the Gram-negative porin family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Outer membrane protein C also known as OmpC protein is a significant component of the outer membrane in Gram-negative bacteria particularly E. coli. This protein with a mass of approximately 40 kDa spans the outer membrane and acts as a porin. It facilitates the selective transport of small molecules and ions into and out of the bacterial cell playing an important role in maintaining the cellular homeostasis. OmpC is abundantly expressed in the outer membrane of E. coli where it forms trimeric complexes contributing to the permeability barrier of the membrane.
- Biological function summary
Outer membrane protein C contributes to the osmotic balance of E. coli cells. It adjusts the permeability of the outer membrane under different environmental conditions such as changes in osmolarity. OmpC forms trimeric structures working in concert with other porins like OmpF to modulate the membrane's permeability to various solutes. This ability to regulate solute passage is important for the bacteria's adaptive responses allowing E. coli to thrive in fluctuating environments.
- Pathways
Outer membrane protein C plays a significant role in the regulation of osmotic stress pathways in E. coli. OmpC alongside porins like OmpF and LamB is an important player in these pathways adapting its expression and function in response to changing osmotic conditions. This regulation is essential for the effective transport of metabolites and ions impacting the larger metabolic networks within the bacterium. It influences pathways involved in nutrient uptake which are vital for bacterial survival and adaptation.
- Associated diseases and disorders
Outer membrane protein C has implications in the pathogenesis of certain infections like urinary tract infections (UTIs) caused by E. coli. Variations in the expression of OmpC along with proteins such as TolC can affect the bacterium’s resistance to antibiotics. This resistance arises from changes in membrane permeability that can influence antibiotic uptake presenting challenges for treatment. Understanding OmpC's role in such infections is essential for developing more effective therapeutic strategies.
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3 product images
SDS-PAGE - Recombinant E. coli Outer membrane protein C (Tagged) (ab225590)
Discontinuous SDS-PAGE (Tris-Glycine gel) (reduced) with 5% enrichment gel and 15% separation gel.
Mass Spectrometry - Recombinant E. coli Outer membrane protein C (Tagged) (ab225590)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab225590 could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) ompC
Mass Spectrometry - Recombinant E. coli Outer membrane protein C (Tagged) (ab225590)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab225590 could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) ompC.
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