Recombinant E. coli Protease 7 protein (His tag) is a Escherichia coli K-12 Full Length protein, in the 21 to 317 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
S T E T L S F T P D N I N A D I S L G T L S G K T K E R V Y L A E E G G R K V S Q L D W K F N N A A I I K G A I N W D L M P Q I S I G A A G W T T L G S R G G N M V D Q D W M D S S N P G T W T D E S R H P D T Q L N Y A N E F D L N I K G W L L N E P N Y R L G L M A G Y Q E S R Y S F T A R G G S Y I Y S S E E G F R D D I G S F P N G E R A I G Y K Q R F K M P Y I G L T G S Y R Y E D F E L G G T F K Y S G W V E S S D N D E H Y D P G K R I T Y R S K V K D Q N Y Y S V A V N A G Y Y V T P N A K V Y V E G A W N R V T N K K G N T S L Y D H N N N T S D Y S K N G A G I E N Y N F I T T A G L K Y T F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Protease that can cleave T7 RNA polymerase, ferric enterobactin receptor protein (FEP), antimicrobial peptide protamine and other proteins. This protease has a specificity for paired basic residues.
Targets
Protease 7
Alternative names
Protease 7, Omptin, Outer membrane protein 3B, Protease A, Protease VII, JW0554, b0565, ompT
Recommended products
Recombinant E. coli Protease 7 protein (His tag) is a Escherichia coli K-12 Full Length protein, in the 21 to 317 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P09169-1
- Animal free
No
- Species
Escherichia coli K-12
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
S T E T L S F T P D N I N A D I S L G T L S G K T K E R V Y L A E E G G R K V S Q L D W K F N N A A I I K G A I N W D L M P Q I S I G A A G W T T L G S R G G N M V D Q D W M D S S N P G T W T D E S R H P D T Q L N Y A N E F D L N I K G W L L N E P N Y R L G L M A G Y Q E S R Y S F T A R G G S Y I Y S S E E G F R D D I G S F P N G E R A I G Y K Q R F K M P Y I G L T G S Y R Y E D F E L G G T F K Y S G W V E S S D N D E H Y D P G K R I T Y R S K V K D Q N Y Y S V A V N A G Y Y V T P N A K V Y V E G A W N R V T N K K G N T S L Y D H N N N T S D Y S K N G A G I E N Y N F I T T A G L K Y T F
- Accession
- Protein length
Full Length
- Predicted molecular weight
37.5 kDa
- Amino acids
21 to 317
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Protease that can cleave T7 RNA polymerase, ferric enterobactin receptor protein (FEP), antimicrobial peptide protamine and other proteins. This protease has a specificity for paired basic residues.
- Sequence similarities
Belongs to the peptidase A26 family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protease 7 also known as PRSS7 is a serine protease enzyme with a molecular mass of around 27 kDa. It belongs to the trypsin-like protease family and exhibits proteolytic activity by cleaving peptide bonds in proteins. This enzyme is mainly expressed in the pancreas and the digestive system. Protease 7 plays a role in regulating various physiological processes by breaking down protein substrates.
- Biological function summary
Protease 7 contributes to the digestion of proteins by facilitating their breakdown into smaller peptides that can be easily absorbed by the body. It is not known to be a part of a larger protein complex but interacts closely with other digestive enzymes enhancing its efficiency in processing food proteins. This functionality supports the maintenance of nutrient balance and optimal digestion within the organism.
- Pathways
The enzymatic activity of Protease 7 integrates into the digestive enzyme pathway working alongside other proteases such as trypsin and chymotrypsin to facilitate protein absorption. It relates to the catabolic process of dietary protein degradation ensuring efficient nutrient assimilation. By interacting with similar enzymes Protease 7 contributes to the broader protein digestion and absorption pathways which are essential for maintaining cellular and systemic homeostasis.
- Associated diseases and disorders
Altered Protease 7 activity has been associated with pancreatic disorders especially pancreatitis where the interruption of normal enzyme activity can lead to tissue inflammation and damage. It also shares a connection with cystic fibrosis-related digestive complications where enzyme imbalance impacts gastrointestinal function. In these conditions the interactions with other pancreatic enzymes become critical as dysregulation can exacerbate disease symptoms and affect digestive health.
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1 product image
SDS-PAGE - Recombinant E. coli Protease 7 protein (His tag) (ab225585)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225585 with 5% enrichment gel and 15% separation gel.
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