JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB225585

Recombinant E. coli Protease 7 protein (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant E. coli Protease 7 protein (His tag) is a Escherichia coli K-12 Full Length protein, in the 21 to 317 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

b0565, JW0554, ompT, Protease 7, Omptin, Outer membrane protein 3B, Protease A, Protease VII

1 Images
SDS-PAGE - Recombinant E. coli Protease 7 protein (His tag) (AB225585)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli Protease 7 protein (His tag) (AB225585)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225585 with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P09169

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF","proteinLength":"Full Length","predictedMolecularWeight":"37.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":317,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P09169","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protease 7 also known as PRSS7 is a serine protease enzyme with a molecular mass of around 27 kDa. It belongs to the trypsin-like protease family and exhibits proteolytic activity by cleaving peptide bonds in proteins. This enzyme is mainly expressed in the pancreas and the digestive system. Protease 7 plays a role in regulating various physiological processes by breaking down protein substrates.
Biological function summary

Protease 7 contributes to the digestion of proteins by facilitating their breakdown into smaller peptides that can be easily absorbed by the body. It is not known to be a part of a larger protein complex but interacts closely with other digestive enzymes enhancing its efficiency in processing food proteins. This functionality supports the maintenance of nutrient balance and optimal digestion within the organism.

Pathways

The enzymatic activity of Protease 7 integrates into the digestive enzyme pathway working alongside other proteases such as trypsin and chymotrypsin to facilitate protein absorption. It relates to the catabolic process of dietary protein degradation ensuring efficient nutrient assimilation. By interacting with similar enzymes Protease 7 contributes to the broader protein digestion and absorption pathways which are essential for maintaining cellular and systemic homeostasis.

Altered Protease 7 activity has been associated with pancreatic disorders especially pancreatitis where the interruption of normal enzyme activity can lead to tissue inflammation and damage. It also shares a connection with cystic fibrosis-related digestive complications where enzyme imbalance impacts gastrointestinal function. In these conditions the interactions with other pancreatic enzymes become critical as dysregulation can exacerbate disease symptoms and affect digestive health.

Specifications

Form

Liquid

General info

Function

Protease that can cleave T7 RNA polymerase, ferric enterobactin receptor protein (FEP), antimicrobial peptide protamine and other proteins. This protease has a specificity for paired basic residues.

Sequence similarities

Belongs to the peptidase A26 family.

Product protocols

Target data

Protease that can cleave T7 RNA polymerase, ferric enterobactin receptor protein (FEP), antimicrobial peptide protamine and other proteins. This protease has a specificity for paired basic residues.
See full target information ompT

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com