Recombinant E. coli RecA protein (Active) is a Escherichia coli K-12 Full Length protein, in the 1 to 353 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, EM, FuncS.
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- Cell 27:515-221981Regulation of SOS functions: purification of E. coli LexA protein and determination of its specific site cleaved by the RecA protein.Applications:FuncSReactive species:Escherichia coliT Horii,T Ogawa,T Nakatani,T Hase,H Matsubara,H OgawaPubMed 6101204
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, EM, FuncS
- Biologically active
- No
Amino acid sequence
M A I D E N K Q K A L A A A L G Q I E K Q F G K G S I M R L G E D R S M D V E T I S T G S L S L D I A L G A G G L P M G R I V E I Y G P E S S G K T T L T L Q V I A A A Q R E G K T C A F I D A E H A L D P I Y A R K L G V D I D N L L C S Q P D T G E Q A L E I C D A L A R S G A V D V I V V D S V A A L T P K A E I E G E I G D S H M G L A A R M M S Q A M R K L A G N L K Q S N T L L I F I N Q I R M K I G V M F G N P E T T T G G N A L K F Y A S V R L D I R R I G A V K E G E N V V G S E T R V K V V K N K I A A P F K Q A E F Q I L Y G E G I N F Y G E L V D L G V K E K L I E K A G A W Y S Y K G E K I G Q G K A N A T A W L K D N P E T A K E I E K K V R E L L L S N P N S T P D F S V D D S E G V A E T N E D F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application EM | Reactivity Reacts | Dilution info - | Notes Facilitate DNA observation by electron microscope due to nucleofilament formation with DNA. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes Functional studies on homologous recombination mechanism and SOS response. |
Associated Products
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Target data
Function
Required for homologous recombination and the bypass of mutagenic DNA lesions by the SOS response. Catalyzes ATP-driven homologous pairing and strand exchange of DNA molecules necessary for DNA recombinational repair. Catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352). Mutations in this gene were selected in directed evolution experiments for resistance to intense ionizing radiation (3000 Gy).
Additional Targets
RecA
Alternative names
lexB, recH, rnmB, tif, umuB, zab, b2699, JW2669, recA, Protein RecA, Recombinase A
Recommended products
Recombinant E. coli RecA protein (Active) is a Escherichia coli K-12 Full Length protein, in the 1 to 353 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, EM, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, EM, FuncS
- Biologically active
- No
- Accession
- P0A7G6-1
- Animal free
- No
- Species
- Escherichia coli K-12
- Concentration
- Storage buffer
pH: 6 - 8.5
Constituents: 50% Glycerol (glycerin, glycerine), 1.1% Potassium chloride, 0.32% Tris-HCl buffer, 0.03% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M A I D E N K Q K A L A A A L G Q I E K Q F G K G S I M R L G E D R S M D V E T I S T G S L S L D I A L G A G G L P M G R I V E I Y G P E S S G K T T L T L Q V I A A A Q R E G K T C A F I D A E H A L D P I Y A R K L G V D I D N L L C S Q P D T G E Q A L E I C D A L A R S G A V D V I V V D S V A A L T P K A E I E G E I G D S H M G L A A R M M S Q A M R K L A G N L K Q S N T L L I F I N Q I R M K I G V M F G N P E T T T G G N A L K F Y A S V R L D I R R I G A V K E G E N V V G S E T R V K V V K N K I A A P F K Q A E F Q I L Y G E G I N F Y G E L V D L G V K E K L I E K A G A W Y S Y K G E K I G Q G K A N A T A W L K D N P E T A K E I E K K V R E L L L S N P N S T P D F S V D D S E G V A E T N E D F
- Accession
- P0A7G6
- Protein length
- Full Length
- Predicted molecular weight
- 36 kDa
- Amino acids
- 1 to 353
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab174091 was over-expressed as a recombinant protein and highly purified by several steps of chromatography.
General info
- Function
Required for homologous recombination and the bypass of mutagenic DNA lesions by the SOS response. Catalyzes ATP-driven homologous pairing and strand exchange of DNA molecules necessary for DNA recombinational repair. Catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352).
- Sequence similarities
Belongs to the RecA family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein RecA also known as protein recA My RecA or BB RecA plays a significant role in DNA repair and homologous recombination. RecA has a molecular mass of approximately 38 kilodaltons and is highly conserved among various species including in bacteria E. coli. It features ATPase activity allowing it to bind and hydrolyze ATP to facilitate its functions. RecA is expressed widely in bacterial cells where it forms a filament with single-stranded DNA to initiate repair processes.
- Biological function summary
RecA performs several critical functions in maintaining genetic stability. It facilitates homologous pairing and strand exchange essential during DNA repair and recombination events. RecA operates as part of a nucleoprotein filament a complex formed during its interaction with single-stranded DNA. Additionally it activates the autocatalytic cleavage of the LexA repressor which induces the SOS response in bacteria following DNA damage. This response includes the upregulation of various DNA repair enzymes contributing to cell survival under stress conditions.
- Pathways
RecA integrates into cellular processes like the SOS response and homologous recombination. In the SOS response pathway it regulates the cleavage of the LexA repressor protein resulting in the induction of DNA repair genes. In homologous recombination RecA mediates the search for homologous sequences and promotes strand invasion leading to genetic exchange. RecA interacts with proteins such as RuvA and RuvB which assist in branch migration and resolution of recombination intermediates.
- Associated diseases and disorders
RecA-related functions can provide insights into bacterial pathogenicity and stress resistance. Deficiencies in RecA function may result in increased susceptibility to DNA damage affecting bacteria's ability to survive under environmental stresses. While not directly linked to human diseases understanding RecA's mechanisms informs the development of antimicrobial strategies targeting bacterial resistance. Proteins involved in similar repair pathways such as Rad51 in eukaryotes offer parallels for comparing DNA repair processes across different organisms and help in understanding bacterial resilience and persistence.
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2 product images
SDS-PAGE - Recombinant E. coli RecA protein (Active) (ab174091)
SDS-PAGE analysis of purified Recombinant E. coli RecA protein (ab174091).
SDS-PAGE - Recombinant E. coli RecA protein (Active) (ab174091)
Polyacrylamide gel electrophoresis of ab174091.
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