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AB174091

Recombinant E. coli RecA protein (Active)

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(1 Publication)

Recombinant E. coli RecA protein (Active) is a Escherichia coli K-12 Full Length protein, in the 1 to 353 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, EM, FuncS.

View Alternative Names

lexB, recH, rnmB, tif, umuB, zab, b2699, JW2669, recA, Protein RecA, Recombinase A

2 Images
SDS-PAGE - Recombinant E. coli RecA protein (Active) (AB174091)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli RecA protein (Active) (AB174091)

SDS-PAGE analysis of purified Recombinant E. coli RecA protein (ab174091).

SDS-PAGE - Recombinant E. coli RecA protein (Active) (AB174091)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli RecA protein (Active) (AB174091)

Polyacrylamide gel electrophoresis of ab174091.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, EM, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The activity of the RecA protein is measured by its ability to hydrolyze ATP when bound to ssDNA, drive strand exchange events, and facilitate autolytic cleavage of regulatory proteins during the SOS response.

Accession

P0A7G6

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

Storage buffer

pH: 6 - 8.5 Constituents: 50% Glycerol (glycerin, glycerine), 1.1% Potassium chloride, 0.32% Tris-HCl buffer, 0.03% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "EM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Facilitate DNA observation by electron microscope due to nucleofilament formation with DNA.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Functional studies on homologous recombination mechanism and SOS response.</p>" } } }
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Sequence info

[{"sequence":"MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGSLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDPIYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEIGDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNSTPDFSVDDSEGVAETNEDF","proteinLength":"Full Length","predictedMolecularWeight":"36 kDa","actualMolecularWeight":null,"aminoAcidEnd":353,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P0A7G6","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein RecA also known as protein recA My RecA or BB RecA plays a significant role in DNA repair and homologous recombination. RecA has a molecular mass of approximately 38 kilodaltons and is highly conserved among various species including in bacteria E. coli. It features ATPase activity allowing it to bind and hydrolyze ATP to facilitate its functions. RecA is expressed widely in bacterial cells where it forms a filament with single-stranded DNA to initiate repair processes.
Biological function summary

RecA performs several critical functions in maintaining genetic stability. It facilitates homologous pairing and strand exchange essential during DNA repair and recombination events. RecA operates as part of a nucleoprotein filament a complex formed during its interaction with single-stranded DNA. Additionally it activates the autocatalytic cleavage of the LexA repressor which induces the SOS response in bacteria following DNA damage. This response includes the upregulation of various DNA repair enzymes contributing to cell survival under stress conditions.

Pathways

RecA integrates into cellular processes like the SOS response and homologous recombination. In the SOS response pathway it regulates the cleavage of the LexA repressor protein resulting in the induction of DNA repair genes. In homologous recombination RecA mediates the search for homologous sequences and promotes strand invasion leading to genetic exchange. RecA interacts with proteins such as RuvA and RuvB which assist in branch migration and resolution of recombination intermediates.

RecA-related functions can provide insights into bacterial pathogenicity and stress resistance. Deficiencies in RecA function may result in increased susceptibility to DNA damage affecting bacteria's ability to survive under environmental stresses. While not directly linked to human diseases understanding RecA's mechanisms informs the development of antimicrobial strategies targeting bacterial resistance. Proteins involved in similar repair pathways such as Rad51 in eukaryotes offer parallels for comparing DNA repair processes across different organisms and help in understanding bacterial resilience and persistence.
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Specifications

Form

Liquid

Additional notes

ab174091 was over-expressed as a recombinant protein and highly purified by several steps of chromatography.

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General info

Function

Required for homologous recombination and the bypass of mutagenic DNA lesions by the SOS response. Catalyzes ATP-driven homologous pairing and strand exchange of DNA molecules necessary for DNA recombinational repair. Catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed : 22412352).. Mutations in this gene were selected in directed evolution experiments for resistance to intense ionizing radiation (3000 Gy).

Sequence similarities

Belongs to the RecA family.

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Product protocols

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Target data

Required for homologous recombination and the bypass of mutagenic DNA lesions by the SOS response. Catalyzes ATP-driven homologous pairing and strand exchange of DNA molecules necessary for DNA recombinational repair. Catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed : 22412352).. Mutations in this gene were selected in directed evolution experiments for resistance to intense ionizing radiation (3000 Gy).
See full target information recA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 27:515-22 PubMed6101204

1981

Regulation of SOS functions: purification of E. coli LexA protein and determination of its specific site cleaved by the RecA protein.

Applications

FuncS

Species

Escherichia coli

T Horii,T Ogawa,T Nakatani,T Hase,H Matsubara,H Ogawa
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