Skip to main content

Recombinant E. coli RecQ DNA Helicase protein (Active) is a Escherichia coli K-12 Full Length protein, in the 1 to 609 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.

This product has no reviews yet! Submit a review
Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Sequence

M A Q A E V L N L E S G A K Q V L Q E T F G Y Q Q F R P G Q E E I I D T V L S G R D C L V V M P T G G G K S L C Y Q I P A L L L N G L T V V V S P L I S L M K D Q V D Q L Q A N G V A A A C L N S T Q T R E Q Q L E V M T G C R T G Q I R L L Y I A P E R L M L D N F L E H L A H W N P V L L A V D E A H C I S Q W G H D F R P E Y A A L G Q L R Q R F P T L P F M A L T A T A D D T T R Q D I V R L L G L N D P L I Q I S S F D R P N I R Y M L M E K F K P L D Q L M R Y V Q E Q R G K S G I I Y C N S R A K V E D T A A R L Q S K G I S A A A Y H A G L E N N V R A D V Q E K F Q R D D L Q I V V A T V A F G M G I N K P N V R F V V H F D I P R N I E S Y Y Q E T G R A G R D G L P A E A M L F Y D P A D M A W L R R C L E E K P Q G Q L Q D I E R H K L N A M G A F A E A Q T C R R L V L L N Y F G E G R Q E P C G N C D I C L D P P K Q Y D G S T D A Q I A L S T I G R V N Q R F G M G Y V V E V I R G A N N Q R I R D Y G H D K L K V Y G M G R D K S H E H W V S V I R Q L I H L G L V T Q N I A Q H S A L Q L T E A A R P V L R G E S S L Q L A V P R I V A L K P K A M Q K S F G G N Y D R K L F A K L R K L R K S I A D E S N V P P Y V V F N D A T L I E M A E Q M P I T A S E M L S V N G V G M R K L E R F G K P F M A L I R A H V D G D D E E

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Shipping To:
United States

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes
See Hishida et at., 2004.

Images

Target data

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Recombinant E. coli RecQ DNA Helicase protein (Active) is a Escherichia coli K-12 Full Length protein, in the 1 to 609 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, FuncS

Biological activity

Unwinding duplex DNA, dependent on ATP. DNA-dependent ATPase.

Predicted molecular weight

68.36 kDa

Amino acids

1 to 609

Sequence

M A Q A E V L N L E S G A K Q V L Q E T F G Y Q Q F R P G Q E E I I D T V L S G R D C L V V M P T G G G K S L C Y Q I P A L L L N G L T V V V S P L I S L M K D Q V D Q L Q A N G V A A A C L N S T Q T R E Q Q L E V M T G C R T G Q I R L L Y I A P E R L M L D N F L E H L A H W N P V L L A V D E A H C I S Q W G H D F R P E Y A A L G Q L R Q R F P T L P F M A L T A T A D D T T R Q D I V R L L G L N D P L I Q I S S F D R P N I R Y M L M E K F K P L D Q L M R Y V Q E Q R G K S G I I Y C N S R A K V E D T A A R L Q S K G I S A A A Y H A G L E N N V R A D V Q E K F Q R D D L Q I V V A T V A F G M G I N K P N V R F V V H F D I P R N I E S Y Y Q E T G R A G R D G L P A E A M L F Y D P A D M A W L R R C L E E K P Q G Q L Q D I E R H K L N A M G A F A E A Q T C R R L V L L N Y F G E G R Q E P C G N C D I C L D P P K Q Y D G S T D A Q I A L S T I G R V N Q R F G M G Y V V E V I R G A N N Q R I R D Y G H D K L K V Y G M G R D K S H E H W V S V I R Q L I H L G L V T Q N I A Q H S A L Q L T E A A R P V L R G E S S L Q L A V P R I V A L K P K A M Q K S F G G N Y D R K L F A K L R K L R K S I A D E S N V P P Y V V F N D A T L I E M A E Q M P I T A S E M L S V N G V G M R K L E R F G K P F M A L I R A H V D G D D E E

Accession
P15043-1
Protein length

Full Length

Animal free

No

Nature

Recombinant

Species

Escherichia coli K-12

Concentration
Loading...
Storage buffer

pH: 6 - 8.5
Constituents: 10% Glycerol (glycerin, glycerine), 0.38% Potassium chloride, 0.316% Tris HCl, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Specifications

Form

Liquid

Additional notes

Highly purified by several steps of chromatography.

General info

Function

Involved in the RecF recombination pathway; its gene expression is under the regulation of the SOS system. It is a DNA helicase.

Sequence similarities

Belongs to the helicase family. RecQ subfamily.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Applications

1) DNA dependent ATPase.

2) DNA helicase specific for various form of DNA structure like branched form and fork.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com