Recombinant E. coli RecQ DNA Helicase protein (Active) is a Escherichia coli K-12 Full Length protein, in the 1 to 609 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.
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Publications
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- PloS one 9:e1132932014Impact of age-associated cyclopurine lesions on DNA repair helicases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrfan Khan et. al.PubMed 25409515
- The Journal of biological chemistry 287:19188-982012DNA repair and replication fork helicases are differentially affected by alkyl phosphotriester lesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAvvaru N Suhasini et. al.PubMed 22500020
- Genes & development 18:1886-972004Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakashi Hishida,Yong-Woon Han,Tatsuya Shibata,Yoshino Kubota,Yoshizumi Ishino,Hiroshi Iwasaki,Hideo ShinagawaPubMed 15289460
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A Q A E V L N L E S G A K Q V L Q E T F G Y Q Q F R P G Q E E I I D T V L S G R D C L V V M P T G G G K S L C Y Q I P A L L L N G L T V V V S P L I S L M K D Q V D Q L Q A N G V A A A C L N S T Q T R E Q Q L E V M T G C R T G Q I R L L Y I A P E R L M L D N F L E H L A H W N P V L L A V D E A H C I S Q W G H D F R P E Y A A L G Q L R Q R F P T L P F M A L T A T A D D T T R Q D I V R L L G L N D P L I Q I S S F D R P N I R Y M L M E K F K P L D Q L M R Y V Q E Q R G K S G I I Y C N S R A K V E D T A A R L Q S K G I S A A A Y H A G L E N N V R A D V Q E K F Q R D D L Q I V V A T V A F G M G I N K P N V R F V V H F D I P R N I E S Y Y Q E T G R A G R D G L P A E A M L F Y D P A D M A W L R R C L E E K P Q G Q L Q D I E R H K L N A M G A F A E A Q T C R R L V L L N Y F G E G R Q E P C G N C D I C L D P P K Q Y D G S T D A Q I A L S T I G R V N Q R F G M G Y V V E V I R G A N N Q R I R D Y G H D K L K V Y G M G R D K S H E H W V S V I R Q L I H L G L V T Q N I A Q H S A L Q L T E A A R P V L R G E S S L Q L A V P R I V A L K P K A M Q K S F G G N Y D R K L F A K L R K L R K S I A D E S N V P P Y V V F N D A T L I E M A E Q M P I T A S E M L S V N G V G M R K L E R F G K P F M A L I R A H V D G D D E E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes See Hishida et at., 2004. |
Target data
Function
An ATP-dependent DNA helicase which unwinds DNA in a 3'-5' direction (PubMed:12771204, PubMed:16084389, PubMed:2164680, PubMed:9553043). ATPase activity is stimulated by ssDNA but only very poorly by dsDNA (PubMed:2164680). Binds to and unwinds a wide variety of substrates including 3- and 4-way junctions (including Holliday junctions), flayed duplexes, 5'- and 3'-overhangs and blunt end duplexes (PubMed:19151156, PubMed:9553043). Unwinds G-quadruplex DNA (PubMed:11292849). Unlike yeast SGS1 or human BLM, is equally active on dsDNA versus G-quadruplex substrates (PubMed:11292849). Can act as an initiator during homologous recombination; unwinds a linear dsDNA substrate which can be used by RecA to initiate homologous DNA pairing as well as dissociation (PubMed:9553043). The identity of a few residues in the C-terminal HRDC domain influences the type of DNA substrate bound (PubMed:16084389). Involved in the RecF recombination pathway (PubMed:2993821).
Additional Targets
RecQ DNA Helicase
Alternative names
b3822, JW5855, recQ, ATP-dependent DNA helicase RecQ, DNA 3'-5' helicase RecQ
Recommended products
Recombinant E. coli RecQ DNA Helicase protein (Active) is a Escherichia coli K-12 Full Length protein, in the 1 to 609 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Unwinding duplex DNA, dependent on ATP. DNA-dependent ATPase.
- Accession
- P15043-1
- Animal free
- No
- Species
- Escherichia coli K-12
- Concentration
- Storage buffer
pH: 6 - 8.5
Constituents: 10% Glycerol (glycerin, glycerine), 0.38% Potassium chloride, 0.316% Tris HCl, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M A Q A E V L N L E S G A K Q V L Q E T F G Y Q Q F R P G Q E E I I D T V L S G R D C L V V M P T G G G K S L C Y Q I P A L L L N G L T V V V S P L I S L M K D Q V D Q L Q A N G V A A A C L N S T Q T R E Q Q L E V M T G C R T G Q I R L L Y I A P E R L M L D N F L E H L A H W N P V L L A V D E A H C I S Q W G H D F R P E Y A A L G Q L R Q R F P T L P F M A L T A T A D D T T R Q D I V R L L G L N D P L I Q I S S F D R P N I R Y M L M E K F K P L D Q L M R Y V Q E Q R G K S G I I Y C N S R A K V E D T A A R L Q S K G I S A A A Y H A G L E N N V R A D V Q E K F Q R D D L Q I V V A T V A F G M G I N K P N V R F V V H F D I P R N I E S Y Y Q E T G R A G R D G L P A E A M L F Y D P A D M A W L R R C L E E K P Q G Q L Q D I E R H K L N A M G A F A E A Q T C R R L V L L N Y F G E G R Q E P C G N C D I C L D P P K Q Y D G S T D A Q I A L S T I G R V N Q R F G M G Y V V E V I R G A N N Q R I R D Y G H D K L K V Y G M G R D K S H E H W V S V I R Q L I H L G L V T Q N I A Q H S A L Q L T E A A R P V L R G E S S L Q L A V P R I V A L K P K A M Q K S F G G N Y D R K L F A K L R K L R K S I A D E S N V P P Y V V F N D A T L I E M A E Q M P I T A S E M L S V N G V G M R K L E R F G K P F M A L I R A H V D G D D E E
- Accession
- P15043
- Protein length
- Full Length
- Predicted molecular weight
- 68.36 kDa
- Amino acids
- 1 to 609
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Highly purified by several steps of chromatography.
General info
- Function
An ATP-dependent DNA helicase which unwinds DNA in a 3'-5' direction (PubMed:12771204, PubMed:16084389, PubMed:2164680, PubMed:9553043). ATPase activity is stimulated by ssDNA but only very poorly by dsDNA (PubMed:2164680). Binds to and unwinds a wide variety of substrates including 3- and 4-way junctions (including Holliday junctions), flayed duplexes, 5'- and 3'-overhangs and blunt end duplexes (PubMed:19151156, PubMed:9553043). Unwinds G-quadruplex DNA (PubMed:11292849). Unlike yeast SGS1 or human BLM, is equally active on dsDNA versus G-quadruplex substrates (PubMed:11292849). Can act as an initiator during homologous recombination; unwinds a linear dsDNA substrate which can be used by RecA to initiate homologous DNA pairing as well as dissociation (PubMed:9553043). The identity of a few residues in the C-terminal HRDC domain influences the type of DNA substrate bound (PubMed:16084389). Involved in the RecF recombination pathway (PubMed:2993821).
- Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Applications
1) DNA dependent ATPase.
2) DNA helicase specific for various form of DNA structure like branched form and fork.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RecQ DNA helicase also known as RECQL or simply RecQ is a protein essential for maintaining genomic stability. It unwinds DNA duplexes during processes like replication and repair. RecQ helicase has a molecular mass that varies between species typically around 70-100 kDa. This protein is expressed in various cell types and tissue including human fibroblasts and yeasts. RecQ helicase shares an evolutionary conservation across species highlighting its importance in DNA metabolism.
- Biological function summary
RecQ DNA helicase plays an important role in DNA repair replication and recombination processes. It participates in preventing DNA damage accumulation and ensuring accurate replication. RecQ functions as part of a larger complex involving other DNA repair proteins like topoisomerases and single-strand DNA binding proteins. Some variants of this helicase protein such as those found in E. coli DNA serve as models to understand its enzymatic action and structural dynamics.
- Pathways
The RecQ helicase functions within the homologous recombination and non-homologous end joining pathways both essential for DNA repair. It collaborates closely with proteins like RAD51 and BRCA1 which facilitate error-free DNA repair and genome stability. The helicase protein's unwinding activity is matched with these pathways to ensure that DNA damage is resolved accurately and efficiently reducing the risk of mutagenesis.
- Associated diseases and disorders
Mutations in RecQ helicase genes are linked to disorders like Bloom syndrome and Werner syndrome which involve premature aging and increased cancer risk. The helicase interacts with proteins like WRN and BLM whose dysfunction results in these syndromes. Understanding how RecQ helicase proteins contribute to these disorders helps in designing targeted therapies potentially offering therapeutic intervention for affected individuals.
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SDS-PAGE - Recombinant E. coli RecQ DNA Helicase protein (Active) (ab63813)
SDS-PAGE analysis of Recombinant E. coli RecQ DNA Helicase protein (ab63813).
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