Recombinant E. coli RecQ DNA Helicase protein (Active)
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(3 Publications)
Recombinant E. coli RecQ DNA Helicase protein (Active) is a Escherichia coli K-12 Full Length protein, in the 1 to 609 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.
View Alternative Names
b3822, JW5855, recQ, ATP-dependent DNA helicase RecQ, DNA 3'-5' helicase RecQ
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant E. coli RecQ DNA Helicase protein (Active) (AB63813)
SDS-PAGE analysis of Recombinant E. coli RecQ DNA Helicase protein (ab63813).
Reactivity data
Product details
Applications
1) DNA dependent ATPase.
2) DNA helicase specific for various form of DNA structure like branched form and fork.
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RecQ DNA helicase plays an important role in DNA repair replication and recombination processes. It participates in preventing DNA damage accumulation and ensuring accurate replication. RecQ functions as part of a larger complex involving other DNA repair proteins like topoisomerases and single-strand DNA binding proteins. Some variants of this helicase protein such as those found in E. coli DNA serve as models to understand its enzymatic action and structural dynamics.
Pathways
The RecQ helicase functions within the homologous recombination and non-homologous end joining pathways both essential for DNA repair. It collaborates closely with proteins like RAD51 and BRCA1 which facilitate error-free DNA repair and genome stability. The helicase protein's unwinding activity is matched with these pathways to ensure that DNA damage is resolved accurately and efficiently reducing the risk of mutagenesis.
Specifications
Form
Liquid
Additional notes
Highly purified by several steps of chromatography.
General info
Function
An ATP-dependent DNA helicase which unwinds DNA in a 3'-5' direction (PubMed : 12771204, PubMed : 16084389, PubMed : 2164680, PubMed : 9553043). ATPase activity is stimulated by ssDNA but only very poorly by dsDNA (PubMed : 2164680). Binds to and unwinds a wide variety of substrates including 3- and 4-way junctions (including Holliday junctions), flayed duplexes, 5'- and 3'-overhangs and blunt end duplexes (PubMed : 19151156, PubMed : 9553043). Unwinds G-quadruplex DNA (PubMed : 11292849). Unlike yeast SGS1 or human BLM, is equally active on dsDNA versus G-quadruplex substrates (PubMed : 11292849). Can act as an initiator during homologous recombination; unwinds a linear dsDNA substrate which can be used by RecA to initiate homologous DNA pairing as well as dissociation (PubMed : 9553043). The identity of a few residues in the C-terminal HRDC domain influences the type of DNA substrate bound (PubMed : 16084389). Involved in the RecF recombination pathway (PubMed : 2993821).
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Target data
Publications (3)
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PloS one 9:e113293 PubMed25409515
2014
Applications
Unspecified application
Species
Unspecified reactive species
The Journal of biological chemistry 287:19188-98 PubMed22500020
2012
Applications
Unspecified application
Species
Unspecified reactive species
Genes & development 18:1886-97 PubMed15289460
2004
Applications
Unspecified application
Species
Unspecified reactive species
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