Recombinant E. coli RecQ DNA Helicase protein (Active) is a Escherichia coli K-12 Full Length protein, in the 1 to 609 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.
M A Q A E V L N L E S G A K Q V L Q E T F G Y Q Q F R P G Q E E I I D T V L S G R D C L V V M P T G G G K S L C Y Q I P A L L L N G L T V V V S P L I S L M K D Q V D Q L Q A N G V A A A C L N S T Q T R E Q Q L E V M T G C R T G Q I R L L Y I A P E R L M L D N F L E H L A H W N P V L L A V D E A H C I S Q W G H D F R P E Y A A L G Q L R Q R F P T L P F M A L T A T A D D T T R Q D I V R L L G L N D P L I Q I S S F D R P N I R Y M L M E K F K P L D Q L M R Y V Q E Q R G K S G I I Y C N S R A K V E D T A A R L Q S K G I S A A A Y H A G L E N N V R A D V Q E K F Q R D D L Q I V V A T V A F G M G I N K P N V R F V V H F D I P R N I E S Y Y Q E T G R A G R D G L P A E A M L F Y D P A D M A W L R R C L E E K P Q G Q L Q D I E R H K L N A M G A F A E A Q T C R R L V L L N Y F G E G R Q E P C G N C D I C L D P P K Q Y D G S T D A Q I A L S T I G R V N Q R F G M G Y V V E V I R G A N N Q R I R D Y G H D K L K V Y G M G R D K S H E H W V S V I R Q L I H L G L V T Q N I A Q H S A L Q L T E A A R P V L R G E S S L Q L A V P R I V A L K P K A M Q K S F G G N Y D R K L F A K L R K L R K S I A D E S N V P P Y V V F N D A T L I E M A E Q M P I T A S E M L S V N G V G M R K L E R F G K P F M A L I R A H V D G D D E E
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes See Hishida et at., 2004. |
An ATP-dependent DNA helicase which unwinds DNA in a 3'-5' direction (PubMed:12771204, PubMed:16084389, PubMed:2164680, PubMed:9553043). ATPase activity is stimulated by ssDNA but only very poorly by dsDNA (PubMed:2164680). Binds to and unwinds a wide variety of substrates including 3- and 4-way junctions (including Holliday junctions), flayed duplexes, 5'- and 3'-overhangs and blunt end duplexes (PubMed:19151156, PubMed:9553043). Unwinds G-quadruplex DNA (PubMed:11292849). Unlike yeast SGS1 or human BLM, is equally active on dsDNA versus G-quadruplex substrates (PubMed:11292849). Can act as an initiator during homologous recombination; unwinds a linear dsDNA substrate which can be used by RecA to initiate homologous DNA pairing as well as dissociation (PubMed:9553043). The identity of a few residues in the C-terminal HRDC domain influences the type of DNA substrate bound (PubMed:16084389). Involved in the RecF recombination pathway (PubMed:2993821).
RecQ DNA Helicase
b3822, JW5855, recQ, ATP-dependent DNA helicase RecQ, DNA 3'-5' helicase RecQ
Recombinant E. coli RecQ DNA Helicase protein (Active) is a Escherichia coli K-12 Full Length protein, in the 1 to 609 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.
pH: 6 - 8.5
Constituents: 10% Glycerol (glycerin, glycerine), 0.38% Potassium chloride, 0.316% Tris HCl, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Highly purified by several steps of chromatography.
An ATP-dependent DNA helicase which unwinds DNA in a 3'-5' direction (PubMed:12771204, PubMed:16084389, PubMed:2164680, PubMed:9553043). ATPase activity is stimulated by ssDNA but only very poorly by dsDNA (PubMed:2164680). Binds to and unwinds a wide variety of substrates including 3- and 4-way junctions (including Holliday junctions), flayed duplexes, 5'- and 3'-overhangs and blunt end duplexes (PubMed:19151156, PubMed:9553043). Unwinds G-quadruplex DNA (PubMed:11292849). Unlike yeast SGS1 or human BLM, is equally active on dsDNA versus G-quadruplex substrates (PubMed:11292849). Can act as an initiator during homologous recombination; unwinds a linear dsDNA substrate which can be used by RecA to initiate homologous DNA pairing as well as dissociation (PubMed:9553043). The identity of a few residues in the C-terminal HRDC domain influences the type of DNA substrate bound (PubMed:16084389). Involved in the RecF recombination pathway (PubMed:2993821).
Belongs to the helicase family. RecQ subfamily.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Applications
1) DNA dependent ATPase.
2) DNA helicase specific for various form of DNA structure like branched form and fork.
RecQ DNA helicase also known as RECQL or simply RecQ is a protein essential for maintaining genomic stability. It unwinds DNA duplexes during processes like replication and repair. RecQ helicase has a molecular mass that varies between species typically around 70-100 kDa. This protein is expressed in various cell types and tissue including human fibroblasts and yeasts. RecQ helicase shares an evolutionary conservation across species highlighting its importance in DNA metabolism.
RecQ DNA helicase plays an important role in DNA repair replication and recombination processes. It participates in preventing DNA damage accumulation and ensuring accurate replication. RecQ functions as part of a larger complex involving other DNA repair proteins like topoisomerases and single-strand DNA binding proteins. Some variants of this helicase protein such as those found in E. coli DNA serve as models to understand its enzymatic action and structural dynamics.
The RecQ helicase functions within the homologous recombination and non-homologous end joining pathways both essential for DNA repair. It collaborates closely with proteins like RAD51 and BRCA1 which facilitate error-free DNA repair and genome stability. The helicase protein's unwinding activity is matched with these pathways to ensure that DNA damage is resolved accurately and efficiently reducing the risk of mutagenesis.
Mutations in RecQ helicase genes are linked to disorders like Bloom syndrome and Werner syndrome which involve premature aging and increased cancer risk. The helicase interacts with proteins like WRN and BLM whose dysfunction results in these syndromes. Understanding how RecQ helicase proteins contribute to these disorders helps in designing targeted therapies potentially offering therapeutic intervention for affected individuals.
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SDS-PAGE analysis of Recombinant E. coli RecQ DNA Helicase protein (ab63813).
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