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AB91360

Recombinant E. coli RNase H protein

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Recombinant E. coli RNase H protein is a Escherichia coli K-12 Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

dasF, herA, rnh, sdrA, b0214, JW0204, rnhA, Ribonuclease HI, RNase HI, Ribonuclease H, RNase H

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SDS-PAGE - Recombinant E. coli RNase H protein (AB91360)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant E. coli RNase H protein (AB91360)

SDS-PAGE of E.coli RNase H.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 100,000 units/mg protein. Unit Definition: 1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37°C. Endo- and exo-DNase activities and RNase activity were not detected with 100 U/ml RNaseH in 50 ul reaction at 37°C.

Accession

P0A7Y4

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

Storage buffer

pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.75% Potassium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

To avoid contamination of trace amounts of nucleic acids in BSA, use reaction buffer that does not contain BSA and use RNaseH at higher concentrations.

1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H-labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37°C.

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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P0A7Y4","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Greater than 95% protein determined by SDS-PAGE (CBB staining) Endo- and exo-DNase activities and RNase activity were not detected with 100 U/ml RNaseH in 50 ul reaction at 37 degrees C.

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General info

Function

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer. Involved in production of retron derived msDNA (a branched RNA linked by a 2',5'-phosphodiester bond to a single-stranded DNA). Strain K12 / MG1655 does not have retrons, however they can be expressed in this strain which requires this enzyme (tested with retron Ec86) (PubMed : 7568472).

Sequence similarities

Belongs to the RNase H family.

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Product protocols

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Target data

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer. Involved in production of retron derived msDNA (a branched RNA linked by a 2',5'-phosphodiester bond to a single-stranded DNA). Strain K12 / MG1655 does not have retrons, however they can be expressed in this strain which requires this enzyme (tested with retron Ec86) (PubMed : 7568472).
See full target information rnhA
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Product promise

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