Recombinant E. coli RuvC protein (Active)

Specifications

Form

Liquid

General info

Function

The RuvA-RuvB-RuvC complex processes Holliday junctions during genetic recombination and DNA repair (PubMed : 6374379). Endonuclease that resolves Holliday junction (HJ) intermediates. Cleaves cruciform DNA by making single-stranded nicks across the junction at symmetrical positions within the homologous arms, leaving a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction (PubMed : 10471285, PubMed : 1661673, PubMed : 1758493, PubMed : 1829835, PubMed : 36000732, PubMed : 8001122, PubMed : 8106500, PubMed : 8195150, PubMed : 9000618, PubMed : 9135161, PubMed : 9160752). The consensus cleavage sequence is 5'-(A/T)TT(C>G/A)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange, although there is some flexibility in the position cleaved (PubMed : 10471285, PubMed : 8001122, PubMed : 8195150, PubMed : 9135161). The cleavage reactions can be uncoupled; incision requires the presence of two consensus cleavage sequences, although they do not have to be identical (PubMed : 9135161). The presence of a 5'-phosphate in a half-cut site accelerates cleavage of the second site, ensuring the second cleavage occurs within the lifetime of a single RuvC-HJ complex (PubMed : 19399178). Binds to cruciform DNA in a sequence non-specific manner (PubMed : 10471285, PubMed : 8106500, PubMed : 8195150).. An in vitro resolvase system that forms and processes HJ has been reconstituted with DNA substrates, RuvA, RuvB and RuvC. RuvA-RuvB increases the rate of strand exchange (branch migration), dissociates the RecA filament and allows RuvC to cleave in both orientations at the cruciform junction (PubMed : 10421637, PubMed : 9160752). HJ-RuvA-RuvB-RuvC complexes resolve Holliday junctions and also undergo branch migration, providing evidence for a coupled branch migration/HJ resolution reaction (PubMed : 10421637).

Sequence similarities

Belongs to the RuvC family.