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AB99241

Recombinant E. coli surA protein

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Recombinant E. coli surA protein is a Escherichia coli K-12 Full Length protein, in the 21 to 428 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec, FuncS.

View Alternative Names

b0053, JW0052, surA, Chaperone SurA, Peptidyl-prolyl cis-trans isomerase SurA, Rotamase SurA, Survival protein A, PPIase SurA

1 Images
SDS-PAGE - Recombinant E. coli surA protein (AB99241)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant E. coli surA protein (AB99241)

15% SDS-PAGE showing ab99241 at approximately 47.3kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS, Mass Spec

applications

Biologically active

Yes

Biological activity

Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1 nmole of suc-AAPF-pNA per minute at 37°C in Tris-Hcl pH 8.0 using chymotrypsin.

Accession

P0ABZ6

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAPQVVDKVAAVVNNGVVLESDVDGLMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIANIAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESLAQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIAHSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGESKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGSANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAAQKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN","proteinLength":"Full Length","predictedMolecularWeight":"47.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":428,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P0ABZ6","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True

Specifications

Form

Liquid

Additional notes

ab99241 is purified using conventional chromatography techniques.

General info

Function

Chaperone involved in the correct folding and assembly of outer membrane proteins, such as OmpA, OmpF and LamB (PubMed : 8985185). Recognizes specific patterns of aromatic residues and the orientation of their side chains, which are found more frequently in integral outer membrane proteins (PubMed : 8985185). May act in both early periplasmic and late outer membrane-associated steps of protein maturation (PubMed : 2165476). Essential for the survival of E.coli in stationary phase. Required for pilus biogenesis (PubMed : 16267292).

Product protocols

Target data

Chaperone involved in the correct folding and assembly of outer membrane proteins, such as OmpA, OmpF and LamB (PubMed : 8985185). Recognizes specific patterns of aromatic residues and the orientation of their side chains, which are found more frequently in integral outer membrane proteins (PubMed : 8985185). May act in both early periplasmic and late outer membrane-associated steps of protein maturation (PubMed : 2165476). Essential for the survival of E.coli in stationary phase. Required for pilus biogenesis (PubMed : 16267292).
See full target information surA

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