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AB202171

Recombinant E. coli Tryptophanase protein (His tag N-Terminus)

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Recombinant E. coli Tryptophanase protein (His tag N-Terminus) is a Escherichia coli K-12 Full Length protein, in the 1 to 471 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

ind, b3708, JW3686, tnaA, Tryptophanase, L-tryptophan indole-lyase, TNase

1 Images
SDS-PAGE - Recombinant E. coli Tryptophanase protein (His tag N-Terminus) (AB202171)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli Tryptophanase protein (His tag N-Terminus) (AB202171)

15% SDS-PAGE analysis of 3 μg ab202171.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P0A853

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSEDVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDTGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV","proteinLength":"Full Length","predictedMolecularWeight":"55.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":471,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P0A853","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tryptophanase also known as TnaA is an enzyme that catalyzes the cleavage of L-tryptophan into indole pyruvate and ammonia. The mechanism involves the cofactor pyridoxal phosphate (PLP) for the reaction. The enzyme operates as a homotetramer with a molecular mass of around 200 kDa composed of four identical subunits. Tryptophanase is expressed in diverse bacterial species including Escherichia coli where it resides in the cytoplasm. The presence of tryptophanase can vary depending on environmental conditions such as the availability of tryptophan or other relevant substrates.
Biological function summary

Tryptophanase participates in the catabolism of tryptophan an essential amino acid. It serves to break down tryptophan into key metabolic components contributing to the regulation of tryptophan levels within the cell and the production of indole as a signaling molecule. Tryptophanase does not form part of large protein complexes but it acts in conjunction with other enzymes for efficient tryptophan metabolism. Its activity influences bacterial growth and survival particularly when under nutrient-limited conditions.

Pathways

Tryptophanase plays a role in the tryptophan degradation pathway also known as the tryptophan catabolic pathway. This pathway contributes to energy production and cellular signaling. The pathway works alongside the kynurenine pathway where enzymes such as tryptophan 23-dioxygenase help break down tryptophan in different contexts. Tryptophanase’s production of indole affects signaling pathways by serving as a precursor for various compounds impacting bacterial communication and biofilm formation.

The activity of tryptophanase connects to conditions linked to bacterial metabolism such as certain infections or gut microbiome imbalances. E. coli expressing tryptophanase impacts gut health and can relate to disorders like small intestinal bacterial overgrowth (SIBO). Indole one of tryptophanase's products influences human health by affecting host-pathogen interactions and mucosal inflammation. Additionally other proteins such as those involved in immune response and neurotransmitter regulation can be indirectly influenced by tryptophanase activity through changes in indole concentrations.
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Specifications

Form

Liquid

Additional notes

ab202171 was purified using conventional chromatography techniques.

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General info

Sequence similarities

Belongs to the beta-eliminating lyase family.

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Product protocols

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Target data

See full target information tnaA

Additional targets

Tryptophanase
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