Recombinant EBNA2 protein (Tagged) is a Epstein-barr virus strain ag876 Fragment protein, in the 247 to 454 aa range, expressed in Yeast, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>85% SDS-PAGE
- Expression system
Yeast
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M P T Y Y L A L H G G Q S Y N L I V D T D M S G N P S L S V I P T N P Y Q E Q L S N N P L I Q L Q I V V G E N T G A P A P P Q P P P P P P P P P P P E R R D A W T Q E P L P L D M N P L G S D A S Q G P L A S S I R M L C M A Q Y L L R N A R G Q Q G L L R P L G P Q T R S Q V T L E R Q P V H N P R Q E A P I I L L Q S P A P P R F T P V P M V A L G H T L Q P T P P P R P T L P Q P R I P L I I P P R H T N Q P A T T P P T A P Q R L T L G H Q L S L P P H P P P H Q S T P H C S S D S T G L P P P P T S Y S I P S M T L S P E P L P P P A A P A H P L P G V I Y D Q Q A L P P T P G P P W W P P V R D P T P T T Q T P P T N T K Q G P D Q G Q G R G R W R G R G R S K G R G R M H K L P E P R R P G P D T S S P S M P Q L S P V V S L H Q G Q G P E N S P T P G P S T A G P V C R V T P S A T P D I S P I H E P E S S D S E E P P F L F P S D W Y P P T L E P A E L D E S W E G I F E T T E S H S S D E E N V G G P S K R P R T S T Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays a key role in the activation of the host resting B-cell and stimulation of B-cell proliferation. Acts by up-regulating the expression of viral EBNA1-6, LMP1, LMP2A and LMP2B genes, as well as several host genes including CD21, CD23 and MYC. Activates transcription by acting as an adapter molecule that binds to cellular sequence-specific DNA-binding proteins such as host CBF1, SMARCB1 and SPI1. Once EBNA2 is near promoter sites, its acidic activating domain recruits basal and activation-associated transcription factors TFIIB, TAF40, TFIIH components ERCC2 and ERCC3, and CBP in order to promote transcription. Alternatively, EBNA2 can affect activities of cell cycle regulators and retard cell cycle progression at G2/M phase. It also induces chromosomal instability, by disrupting mitotic checkpoints, multi-nucleation and formation of micronuclei in infected cells (By similarity).
Targets
EBNA2
Alternative names
Epstein-Barr nuclear antigen 2, EBNA-2, EBV nuclear antigen 2, EBNA2, BYRF1
Recommended products
Recombinant EBNA2 protein (Tagged) is a Epstein-barr virus strain ag876 Fragment protein, in the 247 to 454 aa range, expressed in Yeast, with >85% purity and suitable for SDS-PAGE.
Alternative names
Epstein-Barr nuclear antigen 2, EBNA-2, EBV nuclear antigen 2, EBNA2, BYRF1
Key facts
- Purity
>85% SDS-PAGE
- Expression system
Yeast
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q69022-1
- Animal free
No
- Species
Epstein-barr virus strain ag876
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
M P T Y Y L A L H G G Q S Y N L I V D T D M S G N P S L S V I P T N P Y Q E Q L S N N P L I Q L Q I V V G E N T G A P A P P Q P P P P P P P P P P P E R R D A W T Q E P L P L D M N P L G S D A S Q G P L A S S I R M L C M A Q Y L L R N A R G Q Q G L L R P L G P Q T R S Q V T L E R Q P V H N P R Q E A P I I L L Q S P A P P R F T P V P M V A L G H T L Q P T P P P R P T L P Q P R I P L I I P P R H T N Q P A T T P P T A P Q R L T L G H Q L S L P P H P P P H Q S T P H C S S D S T G L P P P P T S Y S I P S M T L S P E P L P P P A A P A H P L P G V I Y D Q Q A L P P T P G P P W W P P V R D P T P T T Q T P P T N T K Q G P D Q G Q G R G R W R G R G R S K G R G R M H K L P E P R R P G P D T S S P S M P Q L S P V V S L H Q G Q G P E N S P T P G P S T A G P V C R V T P S A T P D I S P I H E P E S S D S E E P P F L F P S D W Y P P T L E P A E L D E S W E G I F E T T E S H S S D E E N V G G P S K R P R T S T Q
- Accession
- Protein length
Fragment
- Amino acids
247 to 454
- Nature
Recombinant
Specifications
- Form
Liquid
General info
- Function
Plays a key role in the activation of the host resting B-cell and stimulation of B-cell proliferation. Acts by up-regulating the expression of viral EBNA1-6, LMP1, LMP2A and LMP2B genes, as well as several host genes including CD21, CD23 and MYC. Activates transcription by acting as an adapter molecule that binds to cellular sequence-specific DNA-binding proteins such as host CBF1, SMARCB1 and SPI1. Once EBNA2 is near promoter sites, its acidic activating domain recruits basal and activation-associated transcription factors TFIIB, TAF40, TFIIH components ERCC2 and ERCC3, and CBP in order to promote transcription. Alternatively, EBNA2 can affect activities of cell cycle regulators and retard cell cycle progression at G2/M phase. It also induces chromosomal instability, by disrupting mitotic checkpoints, multi-nucleation and formation of micronuclei in infected cells (By similarity).
- Sequence similarities
Belongs to the herpesviridae EBNA2 family.
- Post-translational modifications
Phosphorylated.
- Subcellular localisation
Host nucleus matrix
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EBNA2 also known as the Epstein-Barr virus nuclear antigen 2 (EBNA-2) plays a critical role in viral protein function. It is a transcriptional activator essential for the latent infection cycle of Epstein-Barr virus (EBV). EBNA2 binds to specific DNA sequences and interacts with other host cellular factors to initiate transcription. The molecular mass of EBNA2 typically is approximately 88 kDa. EBNA2 mainly expresses in EBV-infected B lymphocytes where it contributes to their immortalization and proliferation.
- Biological function summary
EBNA2 is pivotal in the activation of several viral and cellular genes. It forms complexes with other proteins including the cellular transcription factor RBP-Jκ part of the Notch signaling pathway. This interaction is important for altering the expression patterns of both host and viral genes causing changes in the cell cycle and promoting latency. EBNA2 also regulates genes involved in immune response modulation aiding in viral persistence within the host by evading immune surveillance.
- Pathways
EBNA2 impacts transcriptional regulation and cell signaling reversibly. It is integral to the Notch signaling and transcriptional activation pathway related to EBV latency. EBNA2 recruits proteins like CBF1 (RBP-Jκ) a transcription factor that helps mediate cell fate decisions and maintain latency. It modulates pathways involving c-MYC a central regulator of cell growth and influences transcriptional activity with a downstream effect on cellular transformation and proliferation.
- Associated diseases and disorders
EBNA2 is prominently associated with EBV-related malignancies such as Burkitt's lymphoma and Hodgkin's lymphoma. These cancers arise partly from the dysregulated cellular growth induced by EBNA2-mediated transcriptional changes. In these disorders EBNA2 may interact with proteins like LMP1 another EBV-encoded protein that aids in the transformation process promoting oncogenesis and affecting therapy resistance mechanisms.
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SDS-PAGE - Recombinant EBNA2 protein (Tagged) (ab241243)
ab241243 analyzed by SDS-PAGE.
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