Recombinant EBV Nuclear Antigen/EBNA1 protein is a Epstein-Barr Virus (Strain B95-8) Full Length protein, in the 1 to 640 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB, ELISA.
>95% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, WB, ELISA
No
S D E G P G T G P G N G L G E K G D T S G P E G S G G S G P Q R R G G D N H G R G R G R G R G R G G G R P G A P G G S G S G P R H R D G V R R P Q K R P S C I G C K G T H G G T G A G A G A G G A G A G G A G A G G G A G A G G G A G G A G G A G G A G A G G G A G A G G G A G G A G G A G A G G G A G A G G G A G G A G A G G G A G G A G G A G A G G G A G A G G G A G G A G A G G G A G G A G G A G A G G G A G A G G A G G A G G A G A G G A G A G G G A G G A G G A G A G G A G A G G A G A G G A G A G G A G G A G A G G A G G A G A G G A G G A G A G G G A G G A G A G G G A G G A G A G G A G G A G A G G A G G A G A G G A G G A G A G G G A G A G G A G A G G G G R G R G G S G G R G R G G S G G R G R G G S G G R R G R G R E R A R G G S R E R A R G R G R G R G E K R P R S P S S Q S S S S G S P P R R P P P G R R P F F H P V G E A D Y F E Y H Q E G G P D G E P D V P P G A I E Q G P A D D P G E G P S T G P R G Q G D G G R R K K G G W F G K H R G Q G G S N P K F E N I A E G L R A L L A R S H V E R T T D E G T W V A G V F V Y G G S K T S L Y N L R R G T A L A I P Q C R L T P L S R L P F G M A P G P G P Q P G P L R E S I V C Y F M V F L Q T H I F A E V L K D A I K D L V M T K P A P T C N I R V T V C S F D D G V D L P P W F P P M V E G A A A E G D D G D D G D E G G D G D E G E E G Q E
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 0.5 µg/mL | Notes - |
Plays an essential role in replication and partitioning of viral genomic DNA during latent viral infection. During this phase, the circular double-stranded viral DNA undergoes replication once per cell cycle and is efficiently partitioned to the daughter cells. EBNA1 activates the initiation of viral DNA replication through binding to specific sites in the viral latent origin of replication, oriP. Additionally, it governs the segregation of viral episomes by mediating their attachment to host cell metaphase chromosomes. Also activates the transcription of several viral latency genes. Finally, it can counteract the stabilization of host p53/TP53 by host USP7, thereby decreasing apoptosis and increasing host cell survival.
EBV Nuclear Antigen/EBNA1
Epstein-Barr nuclear antigen 1, EBNA-1, EBV nuclear antigen 1, EBNA1, BKRF1
Recombinant EBV Nuclear Antigen/EBNA1 protein is a Epstein-Barr Virus (Strain B95-8) Full Length protein, in the 1 to 640 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB, ELISA.
>95% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, WB, ELISA
No
No
Epstein-Barr Virus (Strain B95-8)
Constituents: Phosphate Buffer, 36% Urea, 5.8% Sodium chloride, 0.1% Poly(oxy-1,2-ethanediyl), .alpha.-tridecyl-.omega.-hydroxy-
S D E G P G T G P G N G L G E K G D T S G P E G S G G S G P Q R R G G D N H G R G R G R G R G R G G G R P G A P G G S G S G P R H R D G V R R P Q K R P S C I G C K G T H G G T G A G A G A G G A G A G G A G A G G G A G A G G G A G G A G G A G G A G A G G G A G A G G G A G G A G G A G A G G G A G A G G G A G G A G A G G G A G G A G G A G A G G G A G A G G G A G G A G A G G G A G G A G G A G A G G G A G A G G A G G A G G A G A G G A G A G G G A G G A G G A G A G G A G A G G A G A G G A G A G G A G G A G A G G A G G A G A G G A G G A G A G G G A G G A G A G G G A G G A G A G G A G G A G A G G A G G A G A G G A G G A G A G G G A G A G G A G A G G G G R G R G G S G G R G R G G S G G R G R G G S G G R R G R G R E R A R G G S R E R A R G R G R G R G E K R P R S P S S Q S S S S G S P P R R P P P G R R P F F H P V G E A D Y F E Y H Q E G G P D G E P D V P P G A I E Q G P A D D P G E G P S T G P R G Q G D G G R R K K G G W F G K H R G Q G G S N P K F E N I A E G L R A L L A R S H V E R T T D E G T W V A G V F V Y G G S K T S L Y N L R R G T A L A I P Q C R L T P L S R L P F G M A P G P G P Q P G P L R E S I V C Y F M V F L Q T H I F A E V L K D A I K D L V M T K P A P T C N I R V T V C S F D D G V D L P P W F P P M V E G A A A E G D D G D D G D E G G D G D E G E E G Q E
Full Length
63 kDa
1 to 640
Recombinant
His tag N-Terminus
Liquid
Plays an essential role in replication and partitioning of viral genomic DNA during latent viral infection. During this phase, the circular double-stranded viral DNA undergoes replication once per cell cycle and is efficiently partitioned to the daughter cells. EBNA1 activates the initiation of viral DNA replication through binding to specific sites in the viral latent origin of replication, oriP. Additionally, it governs the segregation of viral episomes by mediating their attachment to host cell metaphase chromosomes. Also activates the transcription of several viral latency genes. Finally, it can counteract the stabilization of host p53/TP53 by host USP7, thereby decreasing apoptosis and increasing host cell survival.
Belongs to the herpesviridae EBNA1 family.
Host nucleus
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product was previously labelled as EBV Nuclear Antigen
This supplementary information is collated from multiple sources and compiled automatically.
EBV Nuclear Antigen 1 (EBNA1) is a protein expressed by the Epstein-Barr Virus (EBV) also known as Human Herpesvirus 4. It has a molecular weight of approximately 80 kDa and shows expression in latently infected cells. EBNA1 acts as a viral episome-maintenance protein that binds to the EBV genome ensuring viral DNA replication and partitioning during cell division. This protein facilitates the persistent infection of EBV in host cells. Occasionally it is also referred to as EBV EBNA or EBNA-1 protein.
EBNA1 functions as an EBV-specific DNA-binding protein essential for maintaining the viral episome within infected cells. EBNA1 plays a role in gene regulation by interacting with specific DNA sequences called oriP to assist in the replication and maintenance of the viral DNA. It does not form part of a larger protein complex but strongly influences cellular processes through these interactions. EBNA1 ensures the survival and propagation of the virus without immediate cytopathic effects contributing to its long-term persistence.
EBNA1 is central to viral latency and replication pathways in host cells. EBNA1 influences both the EBV lytic and latent cycles maintaining the latency by stabilizing the viral episome within the nucleus of host cells and regulating viral gene expression. It interacts with cellular proteins like USP7 a deubiquitinase important for the regulation of EBNA1 stability and function. These pathways highlight EBNA1's role in the balance between viral latency and active replication.
EBNA1 is closely associated with several EBV-related cancers such as Burkitt's lymphoma and nasopharyngeal carcinoma. The persistent expression of EBNA1 contributes to oncogenic processes by disrupting normal cellular control mechanisms. EBNA1's interaction with p53 a tumor suppressor protein can interfere with the host's cell-cycle regulation and apoptosis allowing for malignant transformation. These interactions illustrate EBNA1's significant role in the pathogenesis of disorders linked to EBV infection.
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