Recombinant EN-TEV Protease (mutated S219N) protein (Tagged-His Tag) is a Full Length protein, in the 2037 to 2073 aa range, expressed in Escherichia coli, with >98% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >98% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
G H H H H H H H G E S L F K G P R D Y N P I S S T I C H L T N E S D G H T T S L Y G I G F G P F I I T N K H L F R R N N G T L L V Q S L H G V F K V K N T T T L Q Q H L I D G R D M I I I R M P K D F P P F P Q K L K F R E P Q R E E R I C L V T T N F Q T K S M S S M V S D T S C T F P S S D G I F W K H W I Q T K D G Q C G S P L V S T R D G F I V G I H S A S N F T N T N N Y F T S V P K N F M E L L T N Q E A Q Q W V S G W R L N A D S V L W G G H K V F M V K P E E P F Q P V K E A T Q L M N R R R R R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Recommended products
Recombinant EN-TEV Protease (mutated S219N) protein (Tagged-His Tag) is a Full Length protein, in the 2037 to 2073 aa range, expressed in Escherichia coli, with >98% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >98% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Complete digestion of 50μg of T7 Tag-HBxAg protein using 1μg TEV enzyme (in 1/50 ratio) in either 3 hours at 37 °C or 12 hours at 4 °C.
The "standard" reaction buffer for TEV protease is 50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA and 1mM DTT. The duration of the cleavage reaction is typically overnight, although lots of cleavage will happen in the first few hours and prolonged incubation times may not lead to proportional increases in cleavage. TEV protease is maximally active at 34 °C, but we recommend performing the digest at room temperature (20 °C) or 4 °C. TEV protease is only three-fold less active at 4 °C than at 20 °C.
Typically, a good rule of thumb for initial test of digestion is 1 μg TEV enzyme per 50 - 100μg target protein ratio. Perform a small-scale reaction first, if possible, to gauge the efficiency of processing. - Animal free
- No
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.015% EDTA
Sequence info
Amino acid sequence
- G H H H H H H H G E S L F K G P R D Y N P I S S T I C H L T N E S D G H T T S L Y G I G F G P F I I T N K H L F R R N N G T L L V Q S L H G V F K V K N T T T L Q Q H L I D G R D M I I I R M P K D F P P F P Q K L K F R E P Q R E E R I C L V T T N F Q T K S M S S M V S D T S C T F P S S D G I F W K H W I Q T K D G Q C G S P L V S T R D G F I V G I H S A S N F T N T N N Y F T S V P K N F M E L L T N Q E A Q Q W V S G W R L N A D S V L W G G H K V F M V K P E E P F Q P V K E A T Q L M N R R R R R
- Accession
- P04517
- Protein length
- Full Length
- Amino acids
- 2037 to 2073
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab134877 is sterile-filtered.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EN-TEV Protease also known simply as TEV protease is a specific proteolytic enzyme commonly utilized for its high specificity in cleaving peptide sequences. Its other popular name is Tobacco Etch Virus NIa protease and it has a molecular mass of approximately 27 kDa. TEV protease is expressed mainly in bacteria such as Escherichia coli when used in laboratory settings for protein preparation. Its ability to cleave at a specific sequence makes it invaluable in protein purification processes.
- Biological function summary
The action of TEV protease involves recognizing and cleaving a specific sequence in fusion proteins allowing for the removal of affinity tags. Though not generally part of a complex its activity is often important for modifying proteins without interfering with their structure or function beyond the cleaved site. By providing a method to separate desired parts of proteins TEV protease enhances the study of protein functions and structures. It also assists in generating authentic recombinant proteins critical for various research applications.
- Pathways
TEV protease's role mainly revolves around its function in post-translational modification processes rather than classical signaling pathways. It facilitates pathways where the removal of fusion tags is necessary for studying protein interactions and conformations. This function does not directly involve TEV protease in cellular metabolic pathways but its activity complements the activity of other proteases like Actev protease and protease assay protocol tools by providing a precise cleavage mechanism necessary for subsequent biochemical analysis and studies.
- Associated diseases and disorders
Although TEV protease does not directly relate to specific diseases it has a significant role in research involving proteins associated with various conditions. For instance in neurodegenerative studies researchers utilize TEV protease to isolate and study proteins potentially connected to disorders such as Alzheimer's disease. The protease substrate generated by its specific cleavage can be studied alongside proteins like tau which are linked to disease pathology. The ability to manipulate protein structures cleanly and efficiently with TEV protease underpins its utility in both clinical research and therapeutic development.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Functional Studies - Recombinant EN-TEV Protease (mutated S219N) protein (Tagged-His Tag) (ab134877)
4-12% SDS-PAGE gel.
M: Protein Marker
Lane 1: N‐terminal tag HBxAg‐11R protein before TEV
enzyme digestion.
Lane 2: N‐terminal tag HBxAg‐11R protein
digested using 1/50 dilution (1µg TEV/ 50µg
target protein at 4°C for overnight reaction) in
buffer 20 mM Tris‐HCl, pH 8.0, 100 mM KCl.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com