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AB238365

Recombinant Endo-beta-1,6-galactanase protein (His tag)

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Recombinant Endo-beta-1,6-galactanase protein (His tag) is a Trichoderma viride Full Length protein, in the 21 to 479 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

6GAL

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SDS-PAGE - Recombinant Endo-beta-1,6-galactanase protein (His tag) (AB238365)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Endo-beta-1,6-galactanase protein (His tag) (AB238365)

ab238365 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

10x His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q76FP5

Animal free

No

Carrier free

No

Species

Trichoderma viride

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"DTTLTIDPTSNWGTWEGWGVSLAWWAKAFGNRDDLASVFFSRNNQAVNGQTLPGLGFNIVRYNAGACSNNSYDGSTMVVSPNIKPSRQMDGFWLDWASSDPSSSSWNWNVDANQRAMLQKAKANGANIFELFSNSPMWWMCNNHNPSGSGSSDNLQSWNYQNHAVYLADIAQHAQQSWRIQFQSVEAFNEPSSSWWTAEGTQEGCHFDVSTMATVIGYLNTELSSRGLSSFVASSDENTYDLAISTWQGFNSSTRNIVKRINVHGYQDGGGRRDTLYSLASQAGKRLWNSEYGDSDASGKSMYQNLLLDFTWLHPTAWVYWQAIDGAGWGLIVGDNDNLTLSSASTKYFVLAQLTRHIRQGMQILTTPDVNTAVAYDAGSQKLVIVTANWGSAQTITFDLTRARTAGSNGATVPRWSTQTGGGDQYRSYTDTKINNGKFSASFSSGQVQTFEVSGVVLQ","proteinLength":"Full Length","predictedMolecularWeight":"56.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":479,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q76FP5","tags":[{"tag":"10x His","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endo-beta-16-galactanase also known as E-B-16-gal is an enzyme that performs hydrolysis of the beta-16-galactan linkages in complex carbohydrates. The enzyme carries a mass of about 40 kDa depending on the organism of origin. This protein exhibits broad expression in various plant tissues as it plays a fundamental role in the breakdown of plant cell wall polymers. It facilitates the conversion of complex polysaccharides into simpler sugars.
Biological function summary

This enzyme functions to degrade rhamnogalacturonan-I a component of pectin within the plant cell wall matrix. Endo-beta-16-galactanase operates as part of the larger pectinase complex which includes other enzymes like polygalacturonase and pectin lyase. Through collaboration within this complex the enzyme contributes significantly to cell wall modification and redevelopment during processes such as growth fruit ripening and plant senescence.

Pathways

Endo-beta-16-galactanase plays a role in the plant cell wall degradation pathway actively participating in remodeling plant structure and facilitating plant growth. It works alongside enzymes like endo-14-beta-glucanase which also modulate the structure of the cell wall. Additionally the enzyme plays a part in the carbohydrate biosynthesis pathway ensuring the cycling of sugars needed for energy and growth.

The activity of endo-beta-16-galactanase can be linked to plant defense mechanisms and responses to pathogen attacks. It influences the integrity of the cell wall with its dysregulation potentially leading to issues with plant pathogenicity. The enzyme connects with proteins like chitinase as both participate in fortifying the plant’s defenses against fungal pathogens. Although it is extensively studied in plant systems its activity and function might have parallels in other biological contexts where it's involved in complex carbohydrate metabolism.
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General info

Function

Hydrolyzes galactooligomers with a degree of polymerization higher than 3. Hydrolyzes radish root arabinogalactan-protein. Does not hydrolyze dextran, arabinan, starch, laminarin, beta-1,4- and beta-1,3-galactans, larch wood arabinogalactan or acid-insoluble polygalacturonic acid.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

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Product protocols

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Target data

Hydrolyzes galactooligomers with a degree of polymerization higher than 3. Hydrolyzes radish root arabinogalactan-protein. Does not hydrolyze dextran, arabinan, starch, laminarin, beta-1,4- and beta-1,3-galactans, larch wood arabinogalactan or acid-insoluble polygalacturonic acid.
See full target information 6GAL
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