Recombinant Enhanced GFP protein (His tag)
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(5 Publications)
Recombinant Enhanced GFP protein (His tag) is a Aequorea victoria Full Length protein, in the 2 to 238 aa range, expressed in Escherichia coli, with >95% purity, = 5 EU/µg endotoxin level and suitable for SDS-PAGE. The predicted molecular weight of ab134853 protein is 33.1 kDa.
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View Alternative Names
Green fluorescent protein, GFP
Reactivity data
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Why is this recommended?
We recommend this product because it’s often used in the same experiment or related research.
We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.
Product details
The ab134853 protein can be used as negative control in any transcription factor protein intracellular delivery experiments.
Incubate the eGFP ab199750 protein in various culture mediums at 2µg/ ml concentration to monitoring intracellular protein delivery efficiency.
Function
The Recombinant Enhanced GFP (eGFP) protein ab134853 is second generation monomeric green fluorescent protein that has improved brightness and photostability.
This recombinant GFP protein was constructed with N-terminal tag of HA2 peptide for efficient endosomal release after intracellular protein delivery, and C-terminal 9 arginine domain / 6His Tag, which will efficiently delivery this recombinant protein intracellularly.
ab199750 have a single excitation peak at about 488 nm, with an emission peak wavelength of 509 nm.
Check out our protein gel staining guide for SDS-PAGE here
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Liquid
Additional notes
ab134853 was expressed in E.coli as soluble protein and was purified using a Ni-NTA column.
General info
Function
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Sequence similarities
Belongs to the GFP family.
Post-translational modifications
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Product protocols
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Target data
Publications (5)
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iScience 25:105675 PubMed36561885
2022
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Nature protocols 17:727-747 PubMed35110739
2022
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Nature 595:303-308 PubMed34108682
2021
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Journal of neurotrauma 36:165-175 PubMed29737232
2018
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Biomicrofluidics 10:024118 PubMed27158286
2016
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