Recombinant Enhanced GFP protein (His tag) is a Aequorea victoria Full Length protein, in the 2 to 238 aa range, expressed in Escherichia coli, with >95% purity, = 5 EU/µg endotoxin level and suitable for SDS-PAGE. The predicted molecular weight of ab134853 protein is 33.1 kDa.
- Save time and ensure accurate results - use our recombinant eGFP protein as a control
M G D I M G E W G N E I F G A I A G F L G V S K G E E L F T G V V P I L V E L D G D V N G H K F S V S G E G E G D A T Y G K L T L K F I C T T G K L P V P W P T L V T T L T Y G V Q C F S R Y P D H M K Q H D F F K S A M P E G Y V Q E R T I F F K D D G N Y K T R A E V K F E G D T L V N R I E L K G I D F K E D G N I L G H K L E Y N Y N S H N V Y I M A D K Q K N G I K V N F K I R H N I E D G S V Q L A D H Y Q Q N T P I G D G P V L L P D N H Y L S T Q S A L S K D P N E K R D H M V L L E F V T A A G I T L G M D E L Y K S R H R R H R Q R S R S R A A A R R R R R R R R R H H H H H H
| Application | Reactivity | Dilution info | Notes |
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Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Green fluorescent protein, GFP
Recombinant Enhanced GFP protein (His tag) is a Aequorea victoria Full Length protein, in the 2 to 238 aa range, expressed in Escherichia coli, with >95% purity, = 5 EU/µg endotoxin level and suitable for SDS-PAGE. The predicted molecular weight of ab134853 protein is 33.1 kDa.
- Save time and ensure accurate results - use our recombinant eGFP protein as a control
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
ab134853 was expressed in E.coli as soluble protein and was purified using a Ni-NTA column.
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Belongs to the GFP family.
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Ensure the validity of your result using our recombinant Enhanced GFP protein (His tag) protein ab134853 as a control in SDS-PAGE.
The ab134853 protein can be used as negative control in any transcription factor protein intracellular delivery experiments.
Incubate the eGFP Recombinant mCherry protein (His tag) ab199750 protein in various culture mediums at 2µg/ ml concentration to monitoring intracellular protein delivery efficiency.
Function
The Recombinant Enhanced GFP (eGFP) protein ab134853 is second generation monomeric green fluorescent protein that has improved brightness and photostability.
This recombinant GFP protein was constructed with N-terminal tag of HA2 peptide for efficient endosomal release after intracellular protein delivery, and C-terminal 9 arginine domain / 6His Tag, which will efficiently delivery this recombinant protein intracellularly.
Recombinant mCherry protein (His tag) ab199750 have a single excitation peak at about 488 nm, with an emission peak wavelength of 509 nm.
Check out our protein gel staining guide for SDS-PAGE here
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