Recombinant Firefly Luciferase protein is a Firefly Full Length protein, in the 1 to 550 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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- BMC cancer 22:8442022Characterization and comparison of human glioblastoma models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia A Schulz,Louis T Rodgers,Richard J Kryscio,Anika M S Hartz,Björn BauerPubMed 35922758
- Biotechnology and bioengineering 118:186-1982020Knockout of the caspase 8-associated protein 2 gene improves recombinant protein expression in HEK293 cells through up-regulation of the cyclin-dependent kinase inhibitor 2A gene.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Abaandou,Ashish K Sharma,Joseph ShiloachPubMed 32910455
- Nature communications 10:762019Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTobias G Schips et. al.PubMed 30622267
- Medical sciences (Basel, Switzerland) 6:2018Knocking out Ornithine Decarboxylase Antizyme 1 () Improves Recombinant Protein Expression in the HEK293 Cell Line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Abaandou et. al.PubMed 29890687
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M M E D A K N I K K G P A P F Y P L E D G T A G E Q L H K A M K R Y A L V P G T I A F T D A H I E V D I T Y A E Y F E M S V R L A E A M K R Y G L N T N H R I V V C S E N S L Q F F M P V L G A L F I G V A V A P A N D I Y N E R E L L N S M G I S Q P T V V F V S K K G L Q K I L N V Q K K L P I I Q K I I I M D S K T D Y Q G F Q S M Y T F V T S H L P P G F N E Y D F V P E S F D R D K T I A L I M N S S G S T G L P K G V A L P H R T A C V R F S H A R D P I F G N Q I I P D T A I L S V V P F H H G F G M F T T L G Y L I C G F R V V L M Y R F E E E L F L R S L Q D Y K I Q S A L L V P T L F S F F A K S T L I D K Y D L S N L H E I A S G G A P L S K E V G E A V A K R F H L P G I R Q G Y G L T E T T S A I L I T P E G D D K P G A V G K V V P F F E A K V V D L D T G K T L G V N Q R G E L C V R G P M I M S G Y V N N P E A T N A L I D K D G W L H S G D I A Y W D E D E H F F I V D R L K S L I K Y K G Y Q V A P A E L E S I L L Q H P N I F D A G V A G L P D D D A G E L P A A V V V L E H G K T M T E K E I V D Y V A S Q V T T A K K L R G G V V F V D E V P K G L T G K L D A R K I R E I L I K A K K G G K I A V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Produces green light with a wavelength of 562 nm.
Alternative names
Luciferin 4-monooxygenase, Luciferase
Recommended products
Recombinant Firefly Luciferase protein is a Firefly Full Length protein, in the 1 to 550 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P08659-1
- Animal free
- No
- Species
- Firefly
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M M E D A K N I K K G P A P F Y P L E D G T A G E Q L H K A M K R Y A L V P G T I A F T D A H I E V D I T Y A E Y F E M S V R L A E A M K R Y G L N T N H R I V V C S E N S L Q F F M P V L G A L F I G V A V A P A N D I Y N E R E L L N S M G I S Q P T V V F V S K K G L Q K I L N V Q K K L P I I Q K I I I M D S K T D Y Q G F Q S M Y T F V T S H L P P G F N E Y D F V P E S F D R D K T I A L I M N S S G S T G L P K G V A L P H R T A C V R F S H A R D P I F G N Q I I P D T A I L S V V P F H H G F G M F T T L G Y L I C G F R V V L M Y R F E E E L F L R S L Q D Y K I Q S A L L V P T L F S F F A K S T L I D K Y D L S N L H E I A S G G A P L S K E V G E A V A K R F H L P G I R Q G Y G L T E T T S A I L I T P E G D D K P G A V G K V V P F F E A K V V D L D T G K T L G V N Q R G E L C V R G P M I M S G Y V N N P E A T N A L I D K D G W L H S G D I A Y W D E D E H F F I V D R L K S L I K Y K G Y Q V A P A E L E S I L L Q H P N I F D A G V A G L P D D D A G E L P A A V V V L E H G K T M T E K E I V D Y V A S Q V T T A K K L R G G V V F V D E V P K G L T G K L D A R K I R E I L I K A K K G G K I A V
- Accession
- P08659
- Protein length
- Full Length
- Predicted molecular weight
- 62.9 kDa
- Amino acids
- 1 to 550
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab100961 is purified using conventional chromatography techniques.
General info
- Function
Produces green light with a wavelength of 562 nm.
- Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family.
- Subcellular localisation
- Peroxisome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Firefly luciferase also known as luc assay or luciferase protein is an enzyme that catalyzes the oxidation of luciferin resulting in bioluminescence. This protein has a molecular mass of approximately 61 kDa and is responsible for the characteristic glow of fireflies. The luciferase expression occurs mainly in the light-producing organs of fireflies such as in the insect Photinus pyralis where it plays an important role in light emission.
- Biological function summary
Firefly luciferase converts chemical energy into light making it valuable for various biological applications as a reporter gene. It participates in the formation of a complex with its substrates luciferin and ATP resulting in the production of a photon. This enzyme is widely used in molecular and cellular biology experiments to monitor gene expression and study protein-protein interactions due to its luminescent properties.
- Pathways
Firefly luciferase functions in the bioluminescent pathway involving the conversion of luciferin to oxyluciferin and light emission. It interfaces with ATP a universal energy carrier which highlights its integration into the cellular energy systems. Firefly luciferase is similar to other oxidative enzymes though it is not directly involved with metabolic pathways like the TCA cycle or glycolysis.
- Associated diseases and disorders
Researchers often utilize firefly luciferase as a tool in tumor studies or infectious disease models. Its luminescence aids in imaging and tracking biological processes in real-time. While firefly luciferase itself is not directly associated with specific diseases the use of firefly agents allows researchers to study disease outcomes assess therapeutic efficacy and understand gene expression profiles related to cancer and viral infections often in connection with proteins such as p53 in cancer research.
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1 product image
SDS-PAGE - Recombinant Firefly Luciferase protein (ab100961)
15% SDS-PAGE showing Mouse IGFBP2 ELISA Kit ab100691 (3 μg) at approximately 62.9 kDa.
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