Recombinant German cockroach Allergen Bla g 4 protein (His tag) is a German cockroach Full Length protein, in the 13 to 182 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
N E D C F R H E S L V P N L D Y E R F R G S W I I A A G T S E A L T Q Y K C W I D R F S Y D D A L V S K Y T D S Q G K N R T T I R G R T K F E G N K F T I D Y N D K G K A F S A P Y S V L A T D Y E N Y A I V E G C P A A A N G H V I Y V Q I R F S V R R F H P K L G D K E M I Q H Y T L D Q V N Q H K K A I E E D L K H F N L K Y E D L H S T C H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
Allergen Bla g 4, Allergen Bla g IV
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Recombinant German cockroach Allergen Bla g 4 protein (His tag) is a German cockroach Full Length protein, in the 13 to 182 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- P54962-1
- Animal free
No
- Species
German cockroach
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
N E D C F R H E S L V P N L D Y E R F R G S W I I A A G T S E A L T Q Y K C W I D R F S Y D D A L V S K Y T D S Q G K N R T T I R G R T K F E G N K F T I D Y N D K G K A F S A P Y S V L A T D Y E N Y A I V E G C P A A A N G H V I Y V Q I R F S V R R F H P K L G D K E M I Q H Y T L D Q V N Q H K K A I E E D L K H F N L K Y E D L H S T C H
- Accession
- Protein length
Full Length
- Predicted molecular weight
35.8 kDa
- Amino acids
13 to 182
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Probable ligand-binding protein.
- Sequence similarities
Belongs to the calycin superfamily. Lipocalin family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The allergen Bla g 4 also known as the cockroach allergen is a glycoprotein with a molecular mass of approximately 21 kDa. Scientists observe its expression in the reproductive tissues of cockroaches specifically Blattella germanica commonly known as the German cockroach. This protein functions as a lipocalin binding and transporting small hydrophobic molecules which plays a role in affecting human allergic responses.
- Biological function summary
This allergen serves as a significant component in the cockroach's ability to provoke immune responses in humans. Studies reveal that Bla g 4 forms part of a larger complex that includes volatile pheromones which cockroaches use for communication and mating. This interaction heightens its potency as an allergenic factor causing asthma and other respiratory allergic reactions when humans inhale or come into contact with cockroach debris.
- Pathways
Bla g 4 interacts in immunological pathways that trigger the human immune response. It directly associates with the TH2 (T-helper type 2) pathway which leads to the production of IgE antibodies. These antibodies link to mast cells and basophils inducing the secretion of histamines and other mediators. Additionally connections exist between Bla g 4 and other cockroach proteins such as Bla g 1 which further influence these allergenic pathways.
- Associated diseases and disorders
Research highlights Bla g 4's involvement in allergenic disorders like asthma and allergic rhinitis. When humans with sensitivities encounter this allergen it contributes significantly to the exacerbation of symptoms. Bla g 4 also relates to proteins involved in inflammatory responses such as histamine receptors intensifying conditions related to cockroach exposure and further driving allergic conditions.
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3 product images
SDS-PAGE - Recombinant German cockroach Allergen Bla g 4 protein (His tag) (ab225565)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225565 with 5% enrichment gel and 15% separation gel.
Mass Spectrometry - Recombinant German cockroach Allergen Bla g 4 protein (His tag) (ab225565)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab225565 could indicate that this peptide derived from E.coli-expressed German cockroach Allergen Bla g 4 protein.
Mass Spectrometry - Recombinant German cockroach Allergen Bla g 4 protein (His tag) (ab225565)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab225565 could indicate that this peptide derived from E.coli-expressed German cockroach Allergen Bla g 4 protein.
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