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AB269037

Recombinant GST-TRK fused gene product-ALK fusion protein (Active)

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Recombinant GST-TRK fused gene product-ALK fusion protein (Active) is a Human Fragment protein, in the 1058 to 1620 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Protein TFG, TRK-fused gene protein, TFG

2 Images
Functional Studies - Recombinant GST-TRK fused gene product-ALK fusion protein (Active) (AB269037)
  • FuncS

Supplier Data

Functional Studies - Recombinant GST-TRK fused gene product-ALK fusion protein (Active) (AB269037)

The specific activity of ab269037 was 28 nmol/min/mg in a kinase assay using IGF1Rtide synthetic peptide substrate.

SDS-PAGE - Recombinant GST-TRK fused gene product-ALK fusion protein (Active) (AB269037)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant GST-TRK fused gene product-ALK fusion protein (Active) (AB269037)

SDS-PAGE analysis of ab269037.

Key facts

Purity

>70% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab269037 was 28 nmol/min/mg in a kinase assay using IGF1Rtide synthetic peptide substrate.

Accession

Q92734

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

GST-TRK fused gene product-ALK fusion protein.
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Sequence info

[{"sequence":"VYRRKHQELQAMQMELQSPEYKLSKLRTSTIMTDYNPNYCFAGKTSSISDLKEVPRKNITLIRGLGHGAFGEVYEGQVSGMPNDPSPLQVAVKTLPEVCSEQDELDFLMEALIISKFNHQNIVRCIGVSLQSLPRFILLELMAGGDLKSFLRETRPRPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTCPGPGRVAKIGDFGMARDIYRASYYRKGGCAMLPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLGYMPYPSKSNQEVLEFVTSGGRMDPPKNCPGPVYRIMTQCWQHQPEDRPNFAIILERIEYCTQDPDVINTALPIEYGPLVEEEEKVPVRPKDPEGVPPLLVSQQAKREEERSPAAPPPLPTTSSGKAAKKPTAAEISVRVPRGPAVEGGHVNMAFSQSNPPSELHKVHGSRNKPTSLWNPTYGSWFTEKPTKKNNPIAKKEPHDRGNLGLEGSCTVPPNVATGRLPGASLLLEPSSLTANMKEVPLFRLRHFPCGNVNYGYQQQGLPLEAATAPGAGHYEDTILKSKNSMNQPGP","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1620,"aminoAcidStart":1058,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9UM73","tags":[]},{"sequence":"MNGQLDLSGKLIIKAQLGEDIRRIPIHNEDITYDELVLMMQRVFRGKLLSNDEVTIKYKDEDGDLITIFDSSDLSFAIQCSRILKLTLFVNGQPRPLESSQVKYLRRELIELRNKVNRLLDSLEPPGEPGPSTNIPENDTVDGREEKSASDSSGKQSTQVMAASMSAFDPLKNQDEINKNVMSAFGLTDDQVS","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":193,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q92734","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ALKTRK fused gene product known as a fusion protein combines parts of the anaplastic lymphoma kinase (ALK) and neurotrophic receptor tyrosine kinase (TRK) genes. The fusion protein has a molecular mass usually in the range of 75–200 kDa dependent on the exact fusion partners and the number of amino acids in the resulting protein. Researchers identify these proteins mainly in cancer cells where they play a role in oncogenic signaling. ALK typically shows expression in the brain and nervous system while TRK proteins express in the nervous system and various other tissues during development.
Biological function summary

The ALKTRK fusion protein participates in abnormal cellular signaling that can lead to uncontrolled cell growth. Normally both ALK and TRK proteins function in neuronal development and signaling but when fused the resulting protein loses normal regulatory control. This fusion protein is not generally part of a larger complex but acts independently to drive oncogenic processes. ALK a receptor tyrosine kinase activates pathways that promote cell proliferation and survival mimicked in the fusion form with TRK proteins.

Pathways

The ALKTRK fusion protein engages primarily in the MAPK/ERK and PI3K/AKT pathways which are critical for cell proliferation and survival. In its normal non-fused state ALK interacts in pathways with proteins like IRS1 and GRB2 while TRK partners with proteins such as SHC and PLC-γ. The fusion form activates similar downstream signaling pathways bypassing normal cellular controls and frequently leading to malignancy.

The ALKTRK fused gene product associates closely with certain cancers particularly non-small cell lung cancer and some pediatric malignancies. This fusion protein plays a significant role in these diseases by driving oncogenic transformation and progression. Specifically TRK fusions have been implicated in various solid tumors while ALK fusions are prominently observed in anaplastic large cell lymphoma. Targeting the ALKTRK fusion protein with specific inhibitors can lead to effective therapies for these cancers.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Plays a role in the normal dynamic function of the endoplasmic reticulum (ER) and its associated microtubules (PubMed : 23479643, PubMed : 27813252). Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus (PubMed : 21478858).

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Product protocols

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Target data

Plays a role in the normal dynamic function of the endoplasmic reticulum (ER) and its associated microtubules (PubMed : 23479643, PubMed : 27813252). Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus (PubMed : 21478858).
See full target information Protein TFG

Additional targets

ALK
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