Recombinant hamster PCSK9 protein (Active) is a Full Length protein, in the 30 to 691 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
Q D E D A E Y E E L M L A L R S Q E D G L A E E E A P H V A T A P F H R C S K E A W R L P G T Y I V V L I D G A Q R L Q I E Q T I H R L Q T Q A A R R G Y V I K V L D I F Y D I L P G F V V K M S S D L L D L A L K L P H V K Y I E E D S L V F G Q S I P W N L D R I I P A G R Q A Q E Y S S S N G S G Q V E V Y L L D T S I Q S D H R E I E G R V T I T D F N S V P E E D G T R F H R Q A S K C D S H G T H L A G V V S G R D A G V A K G T I L H S L R V L N C Q G K G T V S G T L I G L E F I W K S Q L I Q P S G P L V V L L P L A G R Y S R I L N T A C Q H L A S N G V V L V A A A G N F R D D A C L Y S P A S A P E V I T V G A T D V Q D Q P V T L G T L G T N F G R C V D L F A P G K D I I G A S S D C S T C F M S Q S G T S Q A A A H V A G I V V T M L T L E P E L T L A E L R Q R L I H F S T K D V I N M A W F P E D Q R V L T P N L V A T M P P K T H G T G L V A G G Q L L C R T V W S A H S G P T R T A T A T A R C A P G E E L L S C S S F S R S G R R R G D R I E A I G G Q Q V C K A F N A F G G E G V Y A V A R C C L L P R A N C S T H T T P A A R T S L G T H V H C H Q K D H V L T G C S F H W E V E G I G V Q R W A V L R S R H Q P G Q C I G H R E A S A H A S C C H A P G L D C K I K E H G I S G P A E Q V T V A C E A G W T L T G C N V L P G A F M T L G A Y A V D N M C V A R S C A T D T A G R T S E E A I V A A A I C C R S R P S A K A S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant hamster PCSK9 protein (Active) is a Full Length protein, in the 30 to 691 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA against immobilized Human LDL R (High Purity) Protein, Strep Tag, (0.5 μg/well). The linear range is 6-50 ng/mL.
- Animal free
- No
- Reconstitution
- Reconstitute at 200 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- Q D E D A E Y E E L M L A L R S Q E D G L A E E E A P H V A T A P F H R C S K E A W R L P G T Y I V V L I D G A Q R L Q I E Q T I H R L Q T Q A A R R G Y V I K V L D I F Y D I L P G F V V K M S S D L L D L A L K L P H V K Y I E E D S L V F G Q S I P W N L D R I I P A G R Q A Q E Y S S S N G S G Q V E V Y L L D T S I Q S D H R E I E G R V T I T D F N S V P E E D G T R F H R Q A S K C D S H G T H L A G V V S G R D A G V A K G T I L H S L R V L N C Q G K G T V S G T L I G L E F I W K S Q L I Q P S G P L V V L L P L A G R Y S R I L N T A C Q H L A S N G V V L V A A A G N F R D D A C L Y S P A S A P E V I T V G A T D V Q D Q P V T L G T L G T N F G R C V D L F A P G K D I I G A S S D C S T C F M S Q S G T S Q A A A H V A G I V V T M L T L E P E L T L A E L R Q R L I H F S T K D V I N M A W F P E D Q R V L T P N L V A T M P P K T H G T G L V A G G Q L L C R T V W S A H S G P T R T A T A T A R C A P G E E L L S C S S F S R S G R R R G D R I E A I G G Q Q V C K A F N A F G G E G V Y A V A R C C L L P R A N C S T H T T P A A R T S L G T H V H C H Q K D H V L T G C S F H W E V E G I G V Q R W A V L R S R H Q P G Q C I G H R E A S A H A S C C H A P G L D C K I K E H G I S G P A E Q V T V A C E A G W T L T G C N V L P G A F M T L G A Y A V D N M C V A R S C A T D T A G R T S E E A I V A A A I C C R S R P S A K A S
- Protein length
- Full Length
- Amino acids
- 30 to 691
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a protein responsible for the regulation of cholesterol levels by binding to low-density lipoprotein receptors (LDLR) on hepatocytes. This interaction marks the LDLRs for degradation reducing the liver's ability to clear LDL cholesterol from the blood. PCSK9 has a molecular weight of approximately 74 kDa. The protein is mainly expressed in the liver but is also found in the intestine and kidneys. PCSK9 is referred to as NARC-1 standing for neural apoptosis-regulated convertase 1 highlighting its role in the liver's cholesterol management system.
- Biological function summary
PCSK9 influences cholesterol homeostasis by its important role in degrading LDL receptors. It functions independently rather than as part of larger protein complexes. PCSK9 gains particular interest in therapeutic contexts where its inhibition can lead to increased LDLR levels and enhanced clearance of LDL cholesterol. Biotinylated PCSK9 and mouse PCSK9 variants provide significant tools for experimental study. Kits such as the PCSK9 ELISA kit enable detailed measurement of PCSK9 levels in blood samples providing insights into cholesterol metabolism dynamics.
- Pathways
PCSK9 operates within the lipid metabolism pathway and the cholesterol biosynthesis pathway. The protein's activity affects the fate of LDL cholesterol within these pathways. It interacts with proteins such as apolipoprotein B (ApoB) which plays a central role in the structural component of LDL particles. The modulation of these pathways by PCSK9 highlights the significance of its function in maintaining cardiovascular health and managing cholesterol levels.
- Associated diseases and disorders
PCSK9 is closely linked to hypercholesterolemia and coronary artery disease. Mutations in PCSK9 can lead to autosomal dominant hypercholesterolemia due to its effect on LDL receptor degradation. Other proteins such as ApoB and LDLR are involved in these conditions tightly interacting with PCSK9's regulatory function. A better understanding of PCSK9's role offers potential therapeutic targets for cardiovascular disease interventions especially through the development of PCSK9 antibodies and PCSK9 assays that adjust cholesterol levels.
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3 product images
Functional Studies - Recombinant hamster PCSK9 protein (Active) (ab221332)
Immobilized Human LDL R (High Purity) Protein, Strep Tag at 5 μg/mL (100 μl/well) can bind ab221332 with a linear range of 6-50 ng/mL.
SDS-PAGE - Recombinant hamster PCSK9 protein (Active) (ab221332)
SDS-PAGE analysis of reduced ab221332 stained overnight with Coomassie Blue.
The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.9 kDa and 59.0 kDa respectively. The protein migrates as 18 kDa and 65 kDa under reducing (R) condition here due to glycosylation.
Functional Studies - Recombinant hamster PCSK9 protein (Active) (ab221332)
Loaded Human LDL R, Fc Tag on Protein A Biosensor, can bind Hamster PCSK9, His Tag with an affinity constant of 4.09 nM as determined in BLI assay
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