Recombinant Hemagglutinin neuraminidase protein (Tagged) is a Newcastle disease virus strain HER/33 Fragment protein, in the 115 to 473 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
N G A A N N S G C G A P V H D P D Y I G G I G K E L I V D D A S D V T S F Y P S A F Q E H L N F I P A P T T G S G C T R I P S F D I S A T H Y C Y T H N V I L S G C R D H S H S H Q Y L A L G V L R T S A T G R V F F S T L R S I N L D D N Q N R K S C S V S A T P L G C D M L C S K I T E T E E E D Y S S V T P T S M V H G R L G F D G Q Y H E K D L D V I T L F K D W V A N Y P G V G G G S F I D N R V W F P V Y G G L K P N S P S D T V Q E G R Y V I Y K R Y N D T C P D E Q D Y Q I R M A K S S Y K P G R F G G K R V Q Q A I L S I K V S T S L G E D P V L T I P P N T V T L M G A E G R V L T V G T S H F L Y Q R G S S Y F S P A L L Y P M T V N N K T A T L H S P Y T F N A F T R P G S V P C Q A S A R C P N S C V T G V Y T D P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Mediates the viral entry into the host cell together with fusion/F protein. Attaches the virus to sialic acid-containing cell receptors and thereby initiates infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion. Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.
Alternative names
Hemagglutinin-neuraminidase, HN
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Recombinant Hemagglutinin neuraminidase protein (Tagged) is a Newcastle disease virus strain HER/33 Fragment protein, in the 115 to 473 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P35741-1
- Animal free
- No
- Species
- Newcastle disease virus strain HER/33
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- N G A A N N S G C G A P V H D P D Y I G G I G K E L I V D D A S D V T S F Y P S A F Q E H L N F I P A P T T G S G C T R I P S F D I S A T H Y C Y T H N V I L S G C R D H S H S H Q Y L A L G V L R T S A T G R V F F S T L R S I N L D D N Q N R K S C S V S A T P L G C D M L C S K I T E T E E E D Y S S V T P T S M V H G R L G F D G Q Y H E K D L D V I T L F K D W V A N Y P G V G G G S F I D N R V W F P V Y G G L K P N S P S D T V Q E G R Y V I Y K R Y N D T C P D E Q D Y Q I R M A K S S Y K P G R F G G K R V Q Q A I L S I K V S T S L G E D P V L T I P P N T V T L M G A E G R V L T V G T S H F L Y Q R G S S Y F S P A L L Y P M T V N N K T A T L H S P Y T F N A F T R P G S V P C Q A S A R C P N S C V T G V Y T D P
- Accession
- P35741
- Protein length
- Fragment
- Predicted molecular weight
- 44.2 kDa
- Amino acids
- 115 to 473
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Mediates the viral entry into the host cell together with fusion/F protein. Attaches the virus to sialic acid-containing cell receptors and thereby initiates infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion.
- Sequence similarities
Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hemagglutinin neuraminidase often known as HN is a protein complex mechanically combining the functions of both hemagglutinin and neuraminidase activities. This protein resides on the surface of certain viruses particularly within the Paramyxoviridae family like human parainfluenza viruses. The hemagglutinin component assists the virus in attaching to the host cell by binding to sialic acid residues on the cell surface. The neuraminidase activity then helps the release of the newly formed viral particles by cleaving sialic acids preventing the aggregation of virions. The protein usually displays a mass of around 66 kDa with its expression highly specific to the cells infected by the virus.
- Biological function summary
The hemagglutinin neuraminidase protein plays a critical role in viral infectivity and propagation. Its dual functions significantly contribute to both the initial adhesion of the virus to the host cell and the subsequent release of progeny virions. The interaction of hemagglutinin and neuraminidase activities facilitates efficient viral replication making the protein essential for the viral life cycle. It often forms part of a larger protein complex on the viral surface to enhance its multifunctional capabilities assisting in the spread from host to host.
- Pathways
Hemagglutinin neuraminidase actively participates in the sialic acid biosynthesis and interference pathways. The enzymatic removal and binding actions of this protein modulate the sialic acid-rich glycoproteins and glycolipids found on the host cell surface. This process is essential for preventing premature viral aggregation allowing efficient release of newly produced virions. The protein interacts with other viral surface proteins such as the fusion protein (F) in viruses to optimize infective efficiency across host cells integrating into notable synergy within a successful infection pathway.
- Associated diseases and disorders
Hemagglutinin neuraminidase significantly relates to respiratory illnesses caused by paramyxoviruses such as croup in children and certain types of pneumonia. Its role in viral attachment and release makes it an important player in the pathogenesis of these diseases. Additionally the protein connects to disease processes through its interaction with other viral proteins including the aforementioned fusion protein which contributes to the pathophysiology of viral infections by mediating membrane fusion required for viral entry into the host cell. Overall hemagglutinin neuraminidase’s function and interaction with cell surfaces highlight its impact on viral virulence and pathogenicity in respiratory tract conditions.
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SDS-PAGE - Recombinant Hemagglutinin neuraminidase protein (Tagged) (ab236804)
ab236804 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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