Recombinant Hepatitis B Virus Core Antigen protein is a Hepatitis B virus subtype adyw Full Length protein, in the 1 to 183 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, ELISA, WB.
M D I D P Y K E F G A T V E L L S F L P S D F F P S V R D L L D N A S A L Y R E A L E S P E H C S P H H T A L R Q A I L C W G E L M T L A T W V G G N L E D P I S R D L V V S Y V N T N M G L K F R Q L L W F H I S C L T F G R E T V I E Y L V S F G V W I R T P P A Y R P P N A P I L S T L P E T T V V R R R G R S P R R R T P S P R R R R S Q S P R R R R S Q S R E S Q C
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Self assembles to form an icosahedral capsid. Most capsids appear to be large particles with an icosahedral symmetry of T=4 and consist of 240 copies of capsid protein, though a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Entering capsids are transported along microtubules to the nucleus. Phosphorylation of the capsid is thought to induce exposure of nuclear localization signal in the C-terminal portion of the capsid protein that allows binding to the nuclear pore complex via the importin (karyopherin-) alpha and beta. Capsids are imported in intact form through the nuclear pore into the nuclear basket, where it probably binds NUP153. Only capsids that contain the mature viral genome can release the viral DNA and capsid protein into the nucleoplasm. Immature capsids get stuck in the basket. Capsids encapsulate the pre-genomic RNA and the P protein. Pre-genomic RNA is reverse-transcribed into DNA while the capsid is still in the cytoplasm. The capsid can then either be directed to the nucleus, providing more genomes for transcription, or bud through the endoplasmic reticulum to provide new virions.
Hepatitis B Virus Core Antigen, C, C, C, C
Capsid protein, Core antigen, Core protein, HBcAg, p21.5, C
Recombinant Hepatitis B Virus Core Antigen protein is a Hepatitis B virus subtype adyw Full Length protein, in the 1 to 183 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, ELISA, WB.
pH: 7.2 - 7.6
Constituents: 50% Glycerol (glycerin, glycerine), 0.435% Sodium chloride, 0.02% PBS
Purification method is proprietary.
Self assembles to form an icosahedral capsid. Most capsids appear to be large particles with an icosahedral symmetry of T=4 and consist of 240 copies of capsid protein, though a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Entering capsids are transported along microtubules to the nucleus. Phosphorylation of the capsid is thought to induce exposure of nuclear localization signal in the C-terminal portion of the capsid protein that allows binding to the nuclear pore complex via the importin (karyopherin-) alpha and beta. Capsids are imported in intact form through the nuclear pore into the nuclear basket, where it probably binds NUP153. Only capsids that contain the mature viral genome can release the viral DNA and capsid protein into the nucleoplasm. Immature capsids get stuck in the basket. Capsids encapsulate the pre-genomic RNA and the P protein. Pre-genomic RNA is reverse-transcribed into DNA while the capsid is still in the cytoplasm. The capsid can then either be directed to the nucleus, providing more genomes for transcription, or bud through the endoplasmic reticulum to provide new virions.
Belongs to the orthohepadnavirus core antigen family.
Phosphorylated by host SRPK1, SRPK2, and maybe protein kinase C or GAPDH. Phosphorylation is critical for pregenomic RNA packaging. Protein kinase C phosphorylation is stimulated by HBx protein and may play a role in transport of the viral genome to the nucleus at the late step during the viral replication cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reacts strongly with human HBV positive serum.
The Hepatitis B Virus Core Antigen also known as HBV core antigen or Hep B core is an important component of the hepatitis B virus (HBV). This antigen often referred to simply as 'core' forms the nucleocapsid of the virus. The HBV core antigen consists of a protein structure with a mass of approximately 21 kilodaltons. It is primarily expressed within the hepatocytes the liver cells where it plays a pivotal role in viral replication and assembly. The HBV core antigen is also referred to in studies as HBV protein or B core.
The hepatitis B core antigen is integral for the assembly of the viral capsid which protects the viral genome during infection. It functions as part of a larger complex with other viral proteins including the pre-core and envelope proteins to facilitate viral lifecycle processes. This antigen allows for the encapsidation of viral RNA and serves as a scaffold for reverse transcription of the viral genome ensuring replication fidelity and successful infection spread.
The hepatitis B core antigen is essential in the viral replication pathway and the HBV lifecycle pathway. It interacts closely with HBV polymerase during the packaging of viral genome into the capsid. These interactions are important for the transformation of RNA into DNA a critical step in viral maturation. The antigen is related to the HBx protein through these pathways; the HBx protein modulates viral replication and can influence the pathogenesis of the virus.
The hepatitis B core antigen is strongly linked to chronic hepatitis B infection and liver diseases such as cirrhosis and hepatocellular carcinoma. The persistence of the core antigen in infected hepatocytes signifies ongoing viral replication which can lead to liver inflammation and damage. The interaction of the core antigen with immune system components such as anti-HBc antibodies assists in the diagnosis and monitoring of disease progression. It connects to the envelope proteins during the disease process which are important in immune system evasion and chronic infection establishment.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com