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AB256517

Recombinant Hepatitis E Virus ORF2 antigen protein (Fc Chimera)

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Recombinant Hepatitis E Virus ORF2 antigen protein (Fc Chimera) is a Hepatitis E virus human/g1/India/Hyderabad Full Length protein, in the 1 to 660 aa range, expressed in HEK 293 cells, with >95%, suitable for SDS-PAGE.

View Alternative Names

Pro-secreted protein ORF2, Protein ORF2, pORF2, ORF2

1 Images
SDS-PAGE - Recombinant Hepatitis E Virus ORF2 antigen protein (Fc Chimera) (AB256517)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Hepatitis E Virus ORF2 antigen protein (Fc Chimera) (AB256517)

SDS-PAGE analysis of 1.4 μg (Lane 1) and 2.8 μg (Lane 2) of ab256517.

Key facts

Purity

>95% SDS-PAGE

Expression system

HEK 293 cells

Tags

Fc tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q68985

Animal free

No

Carrier free

No

Species

Hepatitis E virus human/g1/India/Hyderabad

Storage buffer

pH: 7 - 8 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGPRPILLLFLMFLPMLLAPPPGQPSGRRRGRRSGGSGGGFWGDRVDSQPFAIPYIHPTNPFAPNVTAAAGAGPRVRQPVRPLGSAWRDQAQRPAAASRRRPTTAGAAPLTAVAPAHDTPPVPDVDSRGAILRRQYNLSTSPLTSSVATGTNLVLYAAPLSPLLPLQDGTNTHIMATEASNYAQYRVARATIRYRPLVPNAVGGYAISISFWPQTTPTPTSVDMNSITSTDVRILVQPGIASELVIPSERLHYRNQGWRSVETSGVAEEEATSGLVMLCIHGSPVNSYTNTPYTGALGLLDFALELEFRNLTPGNTNTRVSRYSSTARHRLRRGADGTAELTTTAATRFMKDLYFTSTNGVGEIGRGIALTLFNLADTLLGGLPTELISSAGGQLFYSRPVVSANGEPTVKLYTSVENAQQDKGIAIPNDIDLGESRVVIQDYDNQHEQDRPTPSPAPSRPFSVLRANDVLWLSLTAAEYDQSTYGSSTGPVYVSDSVTLVNVATGAQAVARSLDWTKVTLDGRPLSTIQQYSKIFFVLPLRGKLSFWEAGTTRPGYPYNYNTTASDQLLVENAAGHRVAISTYTTSLGAGPVSISAVAVLGPHSALALLEDTLDYPARAHTFDDFCPECRPLGLQGCAFQSTVAELQRLKMKVGKTREL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":660,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q68985","tags":[{"tag":"Fc","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Hepatitis E Virus ORF2 antigen also known as the ORF2 protein is the major capsid protein of the hepatitis E virus (HEV). It plays an important role in forming the viral particle and is approximately 60 kDa in mass. ORF2 protein is expressed primarily in infected hepatocytes but viral shedding studies detect it in bodily fluids like feces and blood serum during HEV infection. Viral structural proteins like the ORF2 protein are essential for understanding HEV assembly and transmission.
Biological function summary

The ORF2 protein facilitates essential processes in the HEV life cycle. It encompasses virus attachment entry and eventually the encapsidation of the viral RNA. This protein is involved in forming a protective layer around the viral genetic material playing a critical role in host immune evasion. The ability of ORF2 to form virus-like particles makes it a significant component in vaccine development and serological assays intended for hepatitis E diagnosis.

Pathways

ORF2 protein participates actively in pathways related to HEV capsid assembly and virus-host interaction. Its functions intersect with the immune response evasion mechanisms where it interacts with host cellular proteins to help the virus elude immune detection. The ORF2 is often studied alongside other HEV proteins such as ORF3 which aids in viral particle release reflecting their collaborative roles in ensuring efficient viral propagation and survival within the host.

The Hepatitis E Virus ORF2 antigen is directly linked to hepatitis E often associated with acute liver inflammation. The interaction of ORF2 protein with host immune components is important in the disease's progression leading to symptoms like jaundice and liver dysfunction. Research into ORF2 also examines its relationship with other hepatotropic viruses assessing the cross-reactivity or co-infection potential especially in hepatitis B and C. Understanding these interactions enhances strategies for therapeutic developments and vaccine production against hepatitis E and related viral hepatitis infections.
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Specifications

Form

Liquid

Additional notes

Purified from culture supernatant using Protein G affinity chromatography followed by dialysis.

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General info

Function

Isoform Secreted protein ORF2. Plays a role in the inhibition of host antibody-mediated neutralization without blocking viral cell entry.. Isoform Capsid protein. Forms an icosahedral capsid with a T=1 symmetry and a 34 nm diameter. The capsid is composed of 60 copies linked to each other. Binds to the 5' end of the genomic RNA to mediate genome encapsidation (By similarity). Binds to heparin surface proteoglycans (HSPGs) to mediate viral entry. Additionally, the interactions with host ASGR1 and ASGR2 facilitate viral infection of hepatocytes (By similarity). Inhibits IFN production by blocking host TBK1-induced IRF3 phosphorylation (By similarity). The nuclear form probably modulates host gene expression (By similarity).

Sequence similarities

Belongs to the hepevirus capsid protein family.

Post-translational modifications

Pro-secreted protein ORF2. Cleaved by host protease in the N-terminus.. Isoform Secreted protein ORF2. N-glycosylated.. Isoform Capsid protein. Not N-glycosylated. The C-terminus of the capsid protein ORF2 is truncated in non-enveloped virions shedded in feces, probably due to host proteases.

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Product protocols

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Target data

Isoform Secreted protein ORF2. Plays a role in the inhibition of host antibody-mediated neutralization without blocking viral cell entry.. Isoform Capsid protein. Forms an icosahedral capsid with a T=1 symmetry and a 34 nm diameter. The capsid is composed of 60 copies linked to each other. Binds to the 5' end of the genomic RNA to mediate genome encapsidation (By similarity). Binds to heparin surface proteoglycans (HSPGs) to mediate viral entry. Additionally, the interactions with host ASGR1 and ASGR2 facilitate viral infection of hepatocytes (By similarity). Inhibits IFN production by blocking host TBK1-induced IRF3 phosphorylation (By similarity). The nuclear form probably modulates host gene expression (By similarity).
See full target information ORF2
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