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AB235567

Recombinant Hepcidin protein

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Recombinant Hepcidin protein is a Larimichthys crocea Full Length protein, in the 65 to 85 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Hepcidin, hamp

1 Images
SDS-PAGE - Recombinant Hepcidin protein (AB235567)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Hepcidin protein (AB235567)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab235567.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

A1Z0M0

Animal free

No

Carrier free

No

Species

Larimichthys crocea

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"RCRFCCRCCPRMRGCGICCRF","proteinLength":"Full Length","predictedMolecularWeight":"2.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":85,"aminoAcidStart":65,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"A1Z0M0","tags":[]}]
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Properties and storage information

Form
Liquid
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepcidin also known as Liver-Expressed Antimicrobial Peptide (LEAP) or HEPC is an important regulator of iron homeostasis functioning as a hormone. It consists of a small peptide typically around 25 amino acids in length with a molecular mass of approximately 2.8 kDa. Hepcidin is produced predominantly in the liver and is secreted into the bloodstream where it directly binds to the iron export protein ferroportin. This binding induces ferroportin's internalization and degradation effectively restricting iron release into the circulation and controlling systemic iron levels.
Biological function summary

This hormone plays a critical role in maintaining iron balance within the body by regulating both the absorption of dietary iron in the intestine and the release of stored iron from macrophages and hepatocytes. Hepcidin operates independently and is not part of any larger complex. Its levels fluctuate in response to various stimuli including iron levels inflammation and erythropoietic demand impacting iron transport and storage.

Pathways

Hepcidin is integral to the iron metabolism pathway. It interacts intricately with proteins such as transferrin and ferritin which are involved in iron transport and storage. Hepcidin's function is modulated by the BMP/SMAD signaling pathway as well where its expression is promoted by these signaling proteins. This regulation ensures that iron levels are properly maintained for critical processes such as hemoglobin synthesis and cellular respiration.

Hepcidin is connected to conditions such as anemia of chronic disease and hereditary hemochromatosis. In anemia of chronic disease increased hepcidin levels lead to iron sequestration and restricted availability which contributes to the anemic state. In hereditary hemochromatosis mutations can result in decreased hepcidin activity causing excessive iron accumulation in the body. Through these mechanisms hepcidin's interactions with proteins such as ferroportin and transferrin receptor 2 influence the development and progression of these iron-related disorders.
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General info

Function

Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages (By similarity).. Has very strong antibacterial activity against the marine Gram-negative bacteria V.alginolyticus (MIC=24 uM), V.fluvialis, V.harveyis (MIC=12 uM) and V.parahaemolyticus (MIC=6 uM). Has antibacterial activity against the Gram-negative bacteria A.hydrophila (MIC=6 uM), E.coli (MIC=24 uM), and E.coli BL21(DE3)plysS (MIC=6 uM), and the Gram-positive bacteria B.cereus (MIC=24 uM), B.subtilis (MIC=6 uM), C.glutamicum (MIC=3 uM), M.luteus (MIC=3 uM), M.lysodeikticus, S.aureus (MIC=6 uM) and S.epidermis (MIC=12 uM). Possesses antifungal activity against A.niger (MIC=24 uM), F.graminearum (MIC24 uM) and F.solani (MIC=24 uM), but lacks antifungal activity against the yeasts P.pastoris GS115 and C.albicans.

Sequence similarities

Belongs to the hepcidin family.

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Product protocols

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Target data

Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages (By similarity).. Has very strong antibacterial activity against the marine Gram-negative bacteria V.alginolyticus (MIC=24 uM), V.fluvialis, V.harveyis (MIC=12 uM) and V.parahaemolyticus (MIC=6 uM). Has antibacterial activity against the Gram-negative bacteria A.hydrophila (MIC=6 uM), E.coli (MIC=24 uM), and E.coli BL21(DE3)plysS (MIC=6 uM), and the Gram-positive bacteria B.cereus (MIC=24 uM), B.subtilis (MIC=6 uM), C.glutamicum (MIC=3 uM), M.luteus (MIC=3 uM), M.lysodeikticus, S.aureus (MIC=6 uM) and S.epidermis (MIC=12 uM). Possesses antifungal activity against A.niger (MIC=24 uM), F.graminearum (MIC24 uM) and F.solani (MIC=24 uM), but lacks antifungal activity against the yeasts P.pastoris GS115 and C.albicans.
See full target information Hepcidin
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