Recombinant Herpes simplex virus HSV2 gB protein (His tag) is a Human herpesvirus 2 strain HG52 Fragment protein, in the 23 to 279 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
A P A A P A A P R A S G G V A A T V A A N G G P A S R P P P V P S P A T T K A R K R K T K K P P K R P E A T P P P D A N A T V A A G H A T L R A H L R E I K V E N A D A Q F Y V C P P P T G A T V V Q F E Q P R R C P T R P E G Q N Y T E G I A V V F K E N I A P Y K F K A T M Y Y K D V T V S Q V W F G H R Y S Q F M G I F E D R A P V P F E E V I D K I N T K G V C R S T A K Y V R N N M E T T A F H R D D H E T D M E L K P A K V A T R T S R G W H T T D L K Y N P S R V E A F H R Y G T T V N C I V E E V D A R S V Y P Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Envelope glycoprotein that forms spikes at the surface of virion envelope. Essential for the initial attachment to heparan sulfate moieties of the host cell surface proteoglycans. Involved in fusion of viral and cellular membranes leading to virus entry into the host cell. Following initial binding to its host receptors, membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. May be involved in the fusion between the virion envelope and the outer nuclear membrane during virion egress.
Targets
HSV2 gB
Alternative names
Envelope glycoprotein B, gB, gB, UL27
Recommended products
Recombinant Herpes simplex virus HSV2 gB protein (His tag) is a Human herpesvirus 2 strain HG52 Fragment protein, in the 23 to 279 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P08666-1
- Animal free
No
- Species
Human herpesvirus 2 strain HG52
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
A P A A P A A P R A S G G V A A T V A A N G G P A S R P P P V P S P A T T K A R K R K T K K P P K R P E A T P P P D A N A T V A A G H A T L R A H L R E I K V E N A D A Q F Y V C P P P T G A T V V Q F E Q P R R C P T R P E G Q N Y T E G I A V V F K E N I A P Y K F K A T M Y Y K D V T V S Q V W F G H R Y S Q F M G I F E D R A P V P F E E V I D K I N T K G V C R S T A K Y V R N N M E T T A F H R D D H E T D M E L K P A K V A T R T S R G W H T T D L K Y N P S R V E A F H R Y G T T V N C I V E E V D A R S V Y P Y
- Accession
- Protein length
Fragment
- Predicted molecular weight
44.4 kDa
- Amino acids
23 to 279
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Envelope glycoprotein that forms spikes at the surface of virion envelope. Essential for the initial attachment to heparan sulfate moieties of the host cell surface proteoglycans. Involved in fusion of viral and cellular membranes leading to virus entry into the host cell. Following initial binding to its host receptors, membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. May be involved in the fusion between the virion envelope and the outer nuclear membrane during virion egress.
- Sequence similarities
Belongs to the herpesviridae glycoprotein B family.
- Subcellular localisation
Host endosome membrane
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HSV2 gB also known as glycoprotein B is a glycoprotein found on the surface of Herpes Simplex Virus type 2 (HSV2). It has a molecular mass of approximately 130 kDa. The glycoprotein is expressed on the envelope of the viral particle playing an important role during the viral infection process. It is responsible for mediating the fusion of the viral envelope with host cell membranes allowing the virus to enter the host cell. This makes HSV2 gB an important player in the lifecycle of the virus facilitating infection and subsequent replication within human cells.
- Biological function summary
HSV2 gB is important in the initiation of infection and the spread of the virus within the host organism. It belongs to a group of viral envelope proteins that form a complex interacting with other viral glycoproteins to achieve successful attachment and entry into host cells. The complex includes other glycoproteins such as HSV2 gD and HSV2 gH which work together to mediate membrane fusion and entry functions critical for viral propagation and pathogenicity.
- Pathways
HSV2 gB interacts with cellular pathways involved in host cell entry and immune evasion. Important in viral entry pathways gB collaborates with proteins like HSV2 gL to execute the membrane fusion process efficiently. It is also related to pathways that impact immune system recognition. These pathways facilitate HSV2's ability to establish latency and persistence in the host sometimes leading to reactivation and recurrent infections.
- Associated diseases and disorders
HSV2 gB is a central factor in the pathology of herpetic infections primarily genital herpes. The protein's interaction with host immunity contributes to the characteristic symptoms and clinical manifestation of the disease. Moreover HSV2 gB plays a role in the progression of herpetic encephalitis a severe brain infection. It connects with HSV2 gD and gC proteins which also contribute to the virus's disease-causing potential. Understanding HSV2 gB’s role in these diseases guides the development of vaccines and antiviral therapies.
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SDS-PAGE - Recombinant Herpes simplex virus HSV2 gB protein (His tag) (ab225600)
ab225600 analysed by SDS-PAGE (Tris-Glycine with 5% enrichment gel and 15% separation gel).
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