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Recombinant HIV1 Gag protein (His-tag) is a HIV-1 M:B_MN Fragment protein, in the 136 to 366 aa range, expressed in Escherichia coli, with >=95% purity, <= 0.1 EU/mg endotoxin level and suitable for MS, SDS-PAGE, HPLC.

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Images

Mass Spectrometry - Recombinant HIV1 Gag protein (His-tag) (AB310318), expandable thumbnail
  • SDS-PAGE - Recombinant HIV1 Gag protein (His-tag) (AB310318), expandable thumbnail

Key facts

Purity

>=95% HPLC

Endotoxin level

<= 0.1 EU/mg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

MS, SDS-PAGE, HPLC

Biologically active

No

Amino acid sequence

P I V Q N I Q G Q M V H Q A I S P R T L N A W V K V V E E K A F S P E V I P M F S A L S E G A T P Q D L N T M L N T V G G H Q A A M Q M L K E T I N E E A A E W D R L H P A H A G P I A P G Q M R E P R G S D I A G T T S T L Q E Q I G W M T N N P P I P V G E I Y K R W I I L G L N K I V R M Y S P S S I L D I R Q G P K E P F R D Y V D R F Y K T L R A E Q A S Q E V K N W M T E T L L V Q N A N P D C K T I L K A L G P A A T L E E M M T A C Q G V G G P G H K A R V L

Reactivity data

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

HPLC

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Gag polyproteinMediates, with Gag-Pol polyprotein, the essential events in virion assembly, including binding the plasma membrane, making the protein-protein interactions necessary to create spherical particles, recruiting the viral Env proteins, and packaging the genomic RNA via direct interactions with the RNA packaging sequence (Psi).Matrix protein p17Targets the polyprotein to the plasma membrane via a multipartite membrane-binding signal, that includes its myristoylated N-terminus (By similarity). Matrix protein is part of the pre-integration complex. Implicated in the release from host cell mediated by Vpu. Binds to RNA (By similarity).Capsid protein p24Forms the conical core that encapsulates the genomic RNA-nucleocapsid complex in the virion. Most core are conical, with only 7% tubular. The core is constituted by capsid protein hexamer subunits. The core is disassembled soon after virion entry (By similarity). The capsid promotes immune invasion by cloaking viral DNA from CGAS detection (By similarity). Host restriction factors such as TRIM5-alpha or TRIMCyp bind retroviral capsids and cause premature capsid disassembly, leading to blocks in reverse transcription. Capsid restriction by TRIM5 is one of the factors which restricts HIV-1 to the human species. Host PIN1 apparently facilitates the virion uncoating (By similarity). On the other hand, interactions with PDZD8 or CYPA stabilize the capsid.Nucleocapsid protein p7Encapsulates and protects viral dimeric unspliced genomic RNA (gRNA). Binds these RNAs through its zinc fingers. Acts as a nucleic acid chaperone which is involved in rearangement of nucleic acid secondary structure during gRNA retrotranscription. Also facilitates template switch leading to recombination. As part of the polyprotein, participates in gRNA dimerization, packaging, tRNA incorporation and virion assembly.p6-gagPlays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1.

Alternative names

Recommended products

Recombinant HIV1 Gag protein (His-tag) is a HIV-1 M:B_MN Fragment protein, in the 136 to 366 aa range, expressed in Escherichia coli, with >=95% purity, <= 0.1 EU/mg endotoxin level and suitable for MS, SDS-PAGE, HPLC.

Key facts

Purity

>=95% HPLC

Endotoxin level

<= 0.1 EU/mg

Expression system

Escherichia coli

Applications

MS, SDS-PAGE, HPLC

Accession
P05888-1
Animal free

Yes

Species

HIV-1 M:B_MN

Reconstitution

Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

P I V Q N I Q G Q M V H Q A I S P R T L N A W V K V V E E K A F S P E V I P M F S A L S E G A T P Q D L N T M L N T V G G H Q A A M Q M L K E T I N E E A A E W D R L H P A H A G P I A P G Q M R E P R G S D I A G T T S T L Q E Q I G W M T N N P P I P V G E I Y K R W I I L G L N K I V R M Y S P S S I L D I R Q G P K E P F R D Y V D R F Y K T L R A E Q A S Q E V K N W M T E T L L V Q N A N P D C K T I L K A L G P A A T L E E M M T A C Q G V G G P G H K A R V L

Accession

P05888

Protein length

Fragment

Predicted molecular weight

25.68 kDa

Amino acids

136 to 366

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Lyophilized

Additional notes

SDS-PAGE >= 95%

General info

Function

Gag polyprotein

Sequence similarities

Belongs to the primate lentivirus group gag polyprotein family.

Post-translational modifications

Gag-Pol polyprotein: Specific enzymatic cleavages by the viral protease yield mature proteins.

Subcellular localisation

Host endosome, Host multivesicular body, Host nucleus

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Gag polyprotein also known as the Gag protein or HIV Gag is an essential component of the HIV virus. It is expressed predominantly in host cells infected with HIV-1 and HIV-2. The Gag protein organizes into a structural protein that facilitates viral assembly and release. It typically has a mass of around 55 kDa. Gag proteins localize primarily in the cytoplasm and assemble at the plasma membrane where they form virus-like particles (VLPs).

Biological function summary

The Gag polyprotein serves an essential role in the life cycle of HIV. It forms a major structural component of the virus particle and contributes to the virion's architecture. The Gag protein mediates the packaging of the viral RNA genome into new virus particles and is important for forming the matrix capsid and nucleocapsid of the virus. It is not typically considered part of a larger complex but forms its own multimeric assembly during virus budding.

Pathways

The Gag polyprotein is integral to the viral replication pathway and the process of viral budding from host cells. It interacts with host cell membranes and orchestrates the encapsulation of viral components into new virions. Critical pathways like the proteolytic maturation of viral proteins and assembly incorporate Gag proteins involving other viral proteins such as the Pol protein which helps in the processing and maturation of the viral structure.

Associated diseases and disorders

The Gag protein is directly related to HIV/AIDS. The presence and functionality of the Gag protein enable the virus to replicate within the host contributing to the progression of the disease. The interaction between the Gag protein and other viral components like the Env protein is also essential for the viral entry into new cells which exacerbates the infection process. Efforts to inhibit the Gag polyprotein function are important in controlling HIV proliferation and managing HIV-related disorders.

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2 product images

  • Mass Spectrometry - Recombinant HIV1 Gag protein (His-tag) (ab310318), expandable thumbnail

    Mass Spectrometry - Recombinant HIV1 Gag protein (His-tag) (ab310318)

    Mass determination by ESI-TOF. Predicted MW is 25682.83 +/- 10Da. Observed MW is 25551.87.

  • SDS-PAGE - Recombinant HIV1 Gag protein (His-tag) (ab310318), expandable thumbnail

    SDS-PAGE - Recombinant HIV1 Gag protein (His-tag) (ab310318)

    SDS-PAGE analysis of ab310318

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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