Recombinant HIV1 p55 protein is a HIV-1 M:B_ARV2/SF2 Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.
View Alternative Names
Gag polyprotein, Pr55Gag, gag
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant HIV1 p55 protein (AB63995)
Polyacrylamide gel electrophoresis of ab63995.
The arrows show degradation products.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant HIV1 p55 protein (AB63995)
SDS-PAGE analysis of Recombinant HIV1 p55 protein. As the substrate, Recombinant HIV1 p55 protein (1 μg, ab63995) was used in 20 μl reaction volume. The reaction was carried by incubating at 37℃ for 3 h and stopped by adding SDS-PAGE sample buffer. Lane 1 : no protease,
Lane 2 : 0.16 pg.
Lane 3 : 1.6 pg.
Lane 4 : 16 pg
Lane 5 : 0.16 μg
Lane 6 : 1.6 μg protease.
Note that two degradation bands are observed in the preparation of p55 substrate. In lane 4, p25 band is visible and in lane 5, p13 band is visible.
Reactivity data
Product details
ab63995 has been derived from infectious plasmid clone pNL4-3 and belongs to Group B of HIV-1.
Active as HIV-1 protease substrate
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The HIV-1 p55 protein plays a significant role in the structural integrity and replication cycle of the virus. This polyprotein is part of a larger complex involved in the intracellular assembly and release of viral particles. Its assembly process occurs predominantly at the plasma membrane of the host cell where p55 targets and orchestrates the encapsidation of the viral RNA and other proteins. By doing so it facilitates the budding and maturation of the virus which is critical for sustaining the infection process in the host.
Pathways
The HIV-1 p55 protein integrates into the HIV replication pathway where it interacts with host cell machinery to initiate viral assembly. The protein coordinates with other viral proteins like reverse transcriptase and integrase which are involved in the reverse transcription and integration of the viral genome. Additionally the p55 protein links to the ESCRT pathway a cellular pathway important for membrane fission events including virus budding. Through interactions with proteins such as Tsg101 the p55 protein navigates these host pathways to ensure successful viral replication and spread.
Specifications
Form
Liquid
Additional notes
Purified by several steps of chromatography
General info
Function
Gag polyprotein. Mediates, with Gag-Pol polyprotein, the essential events in virion assembly, including binding the plasma membrane, making the protein-protein interactions necessary to create spherical particles, recruiting the viral Env proteins, and packaging the genomic RNA via direct interactions with the RNA packaging sequence (Psi).. Matrix protein p17. Targets the polyprotein to the plasma membrane via a multipartite membrane-binding signal, that includes its myristoylated N-terminus (By similarity). Matrix protein is part of the pre-integration complex. Implicated in the release from host cell mediated by Vpu. Binds to RNA (By similarity).. Capsid protein p24. Forms the conical core that encapsulates the genomic RNA-nucleocapsid complex in the virion. Most core are conical, with only 7% tubular. The core is constituted by capsid protein hexamer subunits. The core is disassembled soon after virion entry (By similarity). The capsid promotes immune invasion by cloaking viral DNA from CGAS detection (By similarity). Host restriction factors such as TRIM5-alpha or TRIMCyp bind retroviral capsids and cause premature capsid disassembly, leading to blocks in reverse transcription. Capsid restriction by TRIM5 is one of the factors which restricts HIV-1 to the human species. Host PIN1 apparently facilitates the virion uncoating (By similarity). On the other hand, interactions with PDZD8 or CYPA stabilize the capsid (By similarity).. Nucleocapsid protein p7. Encapsulates and protects viral dimeric unspliced genomic RNA (gRNA). Binds these RNAs through its zinc fingers. Acts as a nucleic acid chaperone which is involved in rearangement of nucleic acid secondary structure during gRNA retrotranscription. Also facilitates template switch leading to recombination. As part of the polyprotein, participates in gRNA dimerization, packaging, tRNA incorporation and virion assembly.. p6-gag. Plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1.
Sequence similarities
Belongs to the primate lentivirus group gag polyprotein family.
Post-translational modifications
Gag-Pol polyprotein: Specific enzymatic cleavages by the viral protease yield mature proteins.. Matrix protein p17. Tyrosine phosphorylated presumably in the virion by a host kinase. Phosphorylation is apparently not a major regulator of membrane association.. Capsid protein p24 is phosphorylated possibly by host MAPK1; this phosphorylation is necessary for Pin1-mediated virion uncoating.. Nucleocapsid protein p7 is methylated by host PRMT6, impairing its function by reducing RNA annealing and the initiation of reverse transcription.
Subcellular localisation
Host endosome
Target data
Additional targets
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Product promise
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