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AB119880

Recombinant HPV16 L1 protein

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(5 Publications)

Recombinant HPV16 L1 protein is a Human papillomavirus type 16 L1 Full Length protein, in the 1 to 505 aa range, expressed in Saccharomyces cerevisiae, with >90% purity and suitable for western blot, SDS-PAGE, electron microscopy. The predicted molecular weight of ab119880 protein is 56.2 kDa.

- Save time and ensure accurate results - use our HPV 16 L1 protein (VLP) as a control

View Alternative Names

Major capsid protein L1, L1, HPV-16, HPV-16 capsid, HPV16 capsid protein, HPV16 major capsid protein L1, Human papillomavirus type 16 L1, Human papillomavirus type 16 major capsid protein L1, L1, Major capsid L1 protein, Major capsid protein, Major capsid protein L1

3 Images
Electron Microscopy - Recombinant HPV16 L1 protein (AB119880)
  • EM

Supplier Data

Electron Microscopy - Recombinant HPV16 L1 protein (AB119880)

ab119880 viewed by electron microscopy.

Western blot - Recombinant HPV16 L1 protein (AB119880)
  • WB

Supplier Data

Western blot - Recombinant HPV16 L1 protein (AB119880)

Western blot analysis of ab119880.

Lane 1. MWt marker
Lane 2. ab119808

All lanes:

Western blot - Recombinant HPV16 L1 protein (ab119880)

false

SDS-PAGE - Recombinant HPV16 L1 protein (AB119880)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant HPV16 L1 protein (AB119880)

SDS-PAGE analysis of ab119880.

Lane 1. ab119880
Lane 2. MWt marker

Key facts

Purity

>90% SDS-PAGE

Expression system

Saccharomyces cerevisiae

Tags

Tag free

Applications

WB, SDS-PAGE, EM

applications

Biologically active

No

Accession

P03101

Animal free

No

Carrier free

No

Species

Human papillomavirus type 16

Reconstitution

Reconstitute in 50 µL of water

Storage buffer

Constituents: PBS

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

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Secondary Antibodies

AB125912

Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L

RabMAb|Recombinant

ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (AB125912)

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Primary Antibodies

AB267404

Anti-HPV18 L1 antibody

Western blot - Anti-HPV18 L1 antibody (AB267404)

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Proteins & Peptides

AB283421

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SDS-PAGE - Recombinant Human IGHG1 Protein (AB283421)

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Proteins & Peptides

AB181966

Recombinant Dengue virus 2 NS1 protein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "EM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Use at a concentration of 1 - 2 µg/lane.</p>" } } }
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Product details

Ensure the validity of your result using our recombinant HPV 16 L1 protein (VLP) ab119880 as a positive control in SDS-PAGE and western blot.


Check out our protein gel staining guide for SDS-PAGE here

Check out our western blot protocol for more information here
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Sequence info

[{"sequence":"MSLWLPSEATVYLPPVPVSKVVSTDEYVARTNIYYHAGTSRLLAVGHPYFPIKKPNNNKILVPKVSGLQYRVFRIHLPDPNKFGFPDTSFYNPDTQRLVWACVGVEVGRGQPLGVGISGHPLLNKLDDTENASAYAANAGVDNRECISMDYKQTQLCLIGCKPPIGEHWGKGSPCTNVAVNPGDCPPLELINTVIQDGDMVDTGFGAMDFTTLQANKSEVPLDICTSICKYPDYIKMVSEPYGDSLFFYLRREQMFVRHLFNRAGAVGENVPDDLYIKGSGSTANLASSNYFPTPSGSMVTSDAQIFNKPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMSLCAAISTSETTYKNTNFKEYLRHGEEYDLQFIFQLCKITLTADVMTYIHSMNSTILEDWNFGLQPPPGGTLEDTYRFVTSQAIACQKHTPPAPKEDPLKKYTFWEVNLKEKFSADLDQFPLGRKFLLQAGLKAKPKFTLGKRKATPTTSSTSTTAKRKKRKL","proteinLength":"Full Length","predictedMolecularWeight":"56.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":505,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Saccharomyces cerevisiae ","accessionNumber":"P03101","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Reconstitute for long term storage
False
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Specifications

Form

Lyophilized

Additional notes

ab119880 is purified by ultracentifugation.

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General info

Function

Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.

Sequence similarities

Belongs to the papillomaviridae L1 protein family.

Post-translational modifications

ISGylated by host HERC5, this results in dominant-negative effect on virus infectivity.

Subcellular localisation

Host nucleus

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Product protocols

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Target data

Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.
See full target information Major capsid protein L1

Additional targets

HPV16 L1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:28555 PubMed39558031

2024

Optimizing the production of recombinant human papilloma virus type 52 major capsid protein L1 in Hansenula polymorpha.

Applications

Unspecified application

Species

Unspecified reactive species

Wichittra Phimsen,Natsima Kopitak,Tatpong Boontawon,Thantawat Theeranan,Chuenchit Boonchird,Thunyarat Pongtharangkul

Vaccines 11: PubMed36679860

2022

Chimeric Human Papillomavirus-16 Virus-like Particles Presenting P18I10 and T20 Peptides from HIV-1 Envelope Induce HPV16 and HIV-1-Specific Humoral and T Cell-Mediated Immunity in BALB/c Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Wei Chen,Narcís Saubi,Athina Kilpeläinen,Joan Joseph-Munné

Human vaccines & immunotherapeutics 18:2028514 PubMed35103580

2022

Human papillomavirus infection and seroprevalence among female university students in Mexico.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Pedroza-Gonzalez,Julia Reyes-Reali,Maricela Campos-Solorzano,Eloy Moises Blancas-Diaz,Janik Adriana Tomas-Morales,Aldo Aram Hernandez-Aparicio,Daniel Montes de Oca-Samperio,Efrain Garrido,Gina Stella Garcia-Romo,Claudia Fabiola Mendez-Catala,Patricia Alvarez Ortiz,Janet Sánchez Ramos,Maria Isabel Mendoza-Ramos,Alberto Daniel Saucedo-Campos,Glustein Pozo-Molina

Cell reports 38:110217 PubMed35021101

2022

Mannose-binding lectin and complement mediate follicular localization and enhanced immunogenicity of diverse protein nanoparticle immunogens.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin J Read,Lori Won,John C Kraft,Isaac Sappington,Aereas Aung,Shengwei Wu,Julia Bals,Chengbo Chen,Kelly K Lee,Daniel Lingwood,Neil P King,Darrell J Irvine

Pharmaceutics 13: PubMed34834382

2021

Expression of Chimeric HPV-HIV Protein L1P18 in ; Purification and Characterization of the Virus-like Particles.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiki Eto,Narcís Saubi,Pau Ferrer,Joan Joseph-Munné
View all publications
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