Recombinant HPV16 L1 protein is a Human papillomavirus type 16 Full Length protein, in the 1 to 505 aa range, expressed in Saccharomyces cerevisiae, with >90% purity and suitable for SDS-PAGE, EM, WB.
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- Vaccines 11:2022Chimeric Human Papillomavirus-16 Virus-like Particles Presenting P18I10 and T20 Peptides from HIV-1 Envelope Induce HPV16 and HIV-1-Specific Humoral and T Cell-Mediated Immunity in BALB/c Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Wei Chen,Narcís Saubi,Athina Kilpeläinen,Joan Joseph-MunnéPubMed 36679860
- Human vaccines & immunotherapeutics 18:20285142022Human papillomavirus infection and seroprevalence among female university students in Mexico.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander Pedroza-Gonzalez,Julia Reyes-Reali,Maricela Campos-Solorzano,Eloy Moises Blancas-Diaz,Janik Adriana Tomas-Morales,Aldo Aram Hernandez-Aparicio,Daniel Montes de Oca-Samperio,Efrain Garrido,Gina Stella Garcia-Romo,Claudia Fabiola Mendez-Catala,Patricia Alvarez Ortiz,Janet Sánchez Ramos,Maria Isabel Mendoza-Ramos,Alberto Daniel Saucedo-Campos,Glustein Pozo-MolinaPubMed 35103580
- Cell reports 38:1102172022Mannose-binding lectin and complement mediate follicular localization and enhanced immunogenicity of diverse protein nanoparticle immunogens.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin J Read,Lori Won,John C Kraft,Isaac Sappington,Aereas Aung,Shengwei Wu,Julia Bals,Chengbo Chen,Kelly K Lee,Daniel Lingwood,Neil P King,Darrell J IrvinePubMed 35021101
- Pharmaceutics 13:2021Expression of Chimeric HPV-HIV Protein L1P18 in ; Purification and Characterization of the Virus-like Particles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshiki Eto,Narcís Saubi,Pau Ferrer,Joan Joseph-MunnéPubMed 34834382
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Saccharomyces cerevisiae
- Tags
- Tag free
- Applications
- SDS-PAGE, EM, WB
- Biologically active
- No
Amino acid sequence
M S L W L P S E A T V Y L P P V P V S K V V S T D E Y V A R T N I Y Y H A G T S R L L A V G H P Y F P I K K P N N N K I L V P K V S G L Q Y R V F R I H L P D P N K F G F P D T S F Y N P D T Q R L V W A C V G V E V G R G Q P L G V G I S G H P L L N K L D D T E N A S A Y A A N A G V D N R E C I S M D Y K Q T Q L C L I G C K P P I G E H W G K G S P C T N V A V N P G D C P P L E L I N T V I Q D G D M V D T G F G A M D F T T L Q A N K S E V P L D I C T S I C K Y P D Y I K M V S E P Y G D S L F F Y L R R E Q M F V R H L F N R A G A V G E N V P D D L Y I K G S G S T A N L A S S N Y F P T P S G S M V T S D A Q I F N K P Y W L Q R A Q G H N N G I C W G N Q L F V T V V D T T R S T N M S L C A A I S T S E T T Y K N T N F K E Y L R H G E E Y D L Q F I F Q L C K I T L T A D V M T Y I H S M N S T I L E D W N F G L Q P P P G G T L E D T Y R F V T S Q A I A C Q K H T P P A P K E D P L K K Y T F W E V N L K E K F S A D L D Q F P L G R K F L L Q A G L K A K P K F T L G K R K A T P T T S S T S T T A K R K K R K L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application EM | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Use at a concentration of 1 - 2 µg/lane. |
Target data
Function
Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.
Additional Targets
HPV16 L1
Alternative names
Major capsid protein L1, L1
Recommended products
Recombinant HPV16 L1 protein is a Human papillomavirus type 16 Full Length protein, in the 1 to 505 aa range, expressed in Saccharomyces cerevisiae, with >90% purity and suitable for SDS-PAGE, EM, WB.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Saccharomyces cerevisiae
- Tags
- Tag free
- Applications
- SDS-PAGE, EM, WB
- Biologically active
- No
- Accession
- P03101-1
- Animal free
- No
- Species
- Human papillomavirus type 16
- Reconstitution
- Reconstitute in 50 µL of water
- Concentration
- Storage buffer
Constituents: 99% PBS
Sequence info
Amino acid sequence
- M S L W L P S E A T V Y L P P V P V S K V V S T D E Y V A R T N I Y Y H A G T S R L L A V G H P Y F P I K K P N N N K I L V P K V S G L Q Y R V F R I H L P D P N K F G F P D T S F Y N P D T Q R L V W A C V G V E V G R G Q P L G V G I S G H P L L N K L D D T E N A S A Y A A N A G V D N R E C I S M D Y K Q T Q L C L I G C K P P I G E H W G K G S P C T N V A V N P G D C P P L E L I N T V I Q D G D M V D T G F G A M D F T T L Q A N K S E V P L D I C T S I C K Y P D Y I K M V S E P Y G D S L F F Y L R R E Q M F V R H L F N R A G A V G E N V P D D L Y I K G S G S T A N L A S S N Y F P T P S G S M V T S D A Q I F N K P Y W L Q R A Q G H N N G I C W G N Q L F V T V V D T T R S T N M S L C A A I S T S E T T Y K N T N F K E Y L R H G E E Y D L Q F I F Q L C K I T L T A D V M T Y I H S M N S T I L E D W N F G L Q P P P G G T L E D T Y R F V T S Q A I A C Q K H T P P A P K E D P L K K Y T F W E V N L K E K F S A D L D Q F P L G R K F L L Q A G L K A K P K F T L G K R K A T P T T S S T S T T A K R K K R K L
- Accession
- P03101
- Protein length
- Full Length
- Predicted molecular weight
- 56.2 kDa
- Amino acids
- 1 to 505
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
ab119880 is purified by ultracentifugation.
General info
- Function
Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.
- Sequence similarities
Belongs to the papillomaviridae L1 protein family.
- Post-translational modifications
ISGylated by host HERC5, this results in dominant-negative effect on virus infectivity.
- Subcellular localisation
- Host nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle, Reconstitute for long term storage
Product promise
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3 product images
SDS-PAGE - Recombinant HPV16 L1 protein (ab119880)
SDS-PAGE analysis of ab119880.
Lane 1. ab119880
Lane 2. MWt marker
Western blot - Recombinant HPV16 L1 protein (ab119880)
Western blot analysis of ab119880.
Lane 1. MWt marker
Lane 2. ab119808All lanes: Western blot - Recombinant HPV16 L1 protein (ab119880)
Electron Microscopy - Recombinant HPV16 L1 protein (ab119880)
ab119880 viewed by electron microscopy.
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