Recombinant HPV18 L1 protein is a Human papillomavirus type 18 Full Length protein, in the 1 to 507 aa range, expressed in Saccharomyces cerevisiae, with >90% purity and suitable for SDS-PAGE, EM, WB.
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- Human vaccines & immunotherapeutics 18:20285142022Human papillomavirus infection and seroprevalence among female university students in Mexico.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander Pedroza-Gonzalez,Julia Reyes-Reali,Maricela Campos-Solorzano,Eloy Moises Blancas-Diaz,Janik Adriana Tomas-Morales,Aldo Aram Hernandez-Aparicio,Daniel Montes de Oca-Samperio,Efrain Garrido,Gina Stella Garcia-Romo,Claudia Fabiola Mendez-Catala,Patricia Alvarez Ortiz,Janet Sánchez Ramos,Maria Isabel Mendoza-Ramos,Alberto Daniel Saucedo-Campos,Glustein Pozo-MolinaPubMed 35103580
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Saccharomyces cerevisiae
- Tags
- Tag free
- Applications
- SDS-PAGE, EM, WB
- Biologically active
- No
Amino acid sequence
M A L W R P S D N T V Y L P P P S V A R V V N T D D Y V T R T S I F Y H A G S S R L L T V G N P Y F R V P A G G G N K Q D I P K V S A Y Q Y R V F R V Q L P D P N K F G L P D T S I Y N P E T Q R L V W A C A G V E I G R G Q P L G V G L S G H P F Y N K L D D T E S S H A A T S N V S E D V R D N V S V D Y K Q T Q L C I L G C A P A I G E H W A K G T A C K S R P L S Q G D C P P L E L K N T V L E D G D M V D T G Y G A M D F S T L Q D T K C E V P L D I C Q S I C K Y P D Y L Q M S A D P Y G D S M F F C L R R E Q L F A R H F W N R A G T M G D T V P Q S L Y I K G T G M R A S P G S C V Y S P S P S G S I V T S D S Q L F N K P Y W L H K A Q G H N N G V C W H N Q L F V T V V D T T R S T N L T I C A S T Q S P V P G Q Y D A T K F K Q Y S R H V E E Y D L Q F I F Q L C T I T L T A D V M S Y I H S M N S S I L E D W N F G V P P P P T T S L V D T Y R F V Q S V A I T C Q K D A A P A E N K D P Y D K L K F W N V D L K E K F S L D L D Q Y P L G R K F L V Q A G L R R K P T I G P R K R S A P S A T T S S K P A K R V R V R A R K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application EM | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Use at a concentration of 1 - 2 µg/lane. |
Target data
Function
Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.
Alternative names
Major capsid protein L1, L1
Recommended products
Recombinant HPV18 L1 protein is a Human papillomavirus type 18 Full Length protein, in the 1 to 507 aa range, expressed in Saccharomyces cerevisiae, with >90% purity and suitable for SDS-PAGE, EM, WB.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Saccharomyces cerevisiae
- Tags
- Tag free
- Applications
- SDS-PAGE, EM, WB
- Biologically active
- No
- Accession
- P06794-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human papillomavirus type 18
- Reconstitution
- Reconstitute in 50 µL of water
- Concentration
- Storage buffer
Constituents: 99% PBS
Sequence info
Amino acid sequence
- M A L W R P S D N T V Y L P P P S V A R V V N T D D Y V T R T S I F Y H A G S S R L L T V G N P Y F R V P A G G G N K Q D I P K V S A Y Q Y R V F R V Q L P D P N K F G L P D T S I Y N P E T Q R L V W A C A G V E I G R G Q P L G V G L S G H P F Y N K L D D T E S S H A A T S N V S E D V R D N V S V D Y K Q T Q L C I L G C A P A I G E H W A K G T A C K S R P L S Q G D C P P L E L K N T V L E D G D M V D T G Y G A M D F S T L Q D T K C E V P L D I C Q S I C K Y P D Y L Q M S A D P Y G D S M F F C L R R E Q L F A R H F W N R A G T M G D T V P Q S L Y I K G T G M R A S P G S C V Y S P S P S G S I V T S D S Q L F N K P Y W L H K A Q G H N N G V C W H N Q L F V T V V D T T R S T N L T I C A S T Q S P V P G Q Y D A T K F K Q Y S R H V E E Y D L Q F I F Q L C T I T L T A D V M S Y I H S M N S S I L E D W N F G V P P P P T T S L V D T Y R F V Q S V A I T C Q K D A A P A E N K D P Y D K L K F W N V D L K E K F S L D L D Q Y P L G R K F L V Q A G L R R K P T I G P R K R S A P S A T T S S K P A K R V R V R A R K
- Protein length
- Full Length
- Predicted molecular weight
- 56.5 kDa
- Amino acids
- 1 to 507
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
ab119881 is purified by ultracentifugation.
General info
- Function
Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.
- Sequence similarities
Belongs to the papillomaviridae L1 protein family.
- Subcellular localisation
- Host nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle, Reconstitute for long term storage
Product promise
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4 product images
SDS-PAGE - Recombinant HPV18 L1 protein (ab119881)
SDS-PAGE analysis of ab119881.
Lane 1. ab119881
Lane 2. MWt marker
Western blot - Recombinant HPV18 L1 protein (ab119881)
Western blot analysis of ab119881.
Lane 1. ab119881
Lane 2. MWt markerAll lanes: Western blot - Recombinant HPV18 L1 protein (ab119881)
Electron Microscopy - Recombinant HPV18 L1 protein (ab119881)
ab119881 viewed by electron microscopy.
SDS-PAGE - Recombinant HPV18 L1 protein (ab119881)
SDS-PAGE analysis of ab119881.
Lane 1. ab119881, 3 µg/lane
Lane 2. MWt marker 11.0, 17.0, 26.0, 34.0, 43.0, 55.0, 72.0, 95.0, 130.0, 170.0 kDa.
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