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AB119882

Recombinant HPV33 L1 protein

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Recombinant HPV33 L1 protein is a human papillomavirus 33 Full Length protein, in the 1 to 499 aa range, expressed in Saccharomyces cerevisiae , with >90%, suitable for SDS-PAGE, EM, WB, ELISA.

View Alternative Names

Major capsid protein L1, L1

2 Images
Electron Microscopy - Recombinant HPV33 L1 protein (AB119882)
  • EM

Unknown

Electron Microscopy - Recombinant HPV33 L1 protein (AB119882)

ab119882 viewed by electron microscopy.

SDS-PAGE - Recombinant HPV33 L1 protein (AB119882)
  • SDS-PAGE

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SDS-PAGE - Recombinant HPV33 L1 protein (AB119882)

SDS-PAGE analysis of ab119882.

Lane 1. ab119882, 0.5 µg/lane
Lane 2. MWt marker 11.0, 17.0, 26.0, 34.0, 43.0, 55.0, 72.0, 95.0, 130.0, 170.0 kDa.

Key facts

Purity

>90% SDS-PAGE

Expression system

Saccharomyces cerevisiae

Tags

Tag free

Applications

WB, ELISA, EM, SDS-PAGE

applications

Biologically active

No

Accession

P06416

Animal free

No

Carrier free

No

Species

human papillomavirus 33

Reconstitution

Reconstitute in PBS, pH 7.6

Storage buffer

Constituents: 99% PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "EM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Use at a concentration of 1 - 2 µg/lane</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"2-4 µg/mL", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"55.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":499,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P06416","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Reconstitute for long term storage
False
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Specifications

Form

Lyophilized

Additional notes

ab119882 is purified by ultracentifugation.

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General info

Function

Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.

Sequence similarities

Belongs to the papillomaviridae L1 protein family.

Subcellular localisation

Host nucleus

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Product protocols

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Target data

Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.
See full target information Major capsid protein L1

Additional targets

HPV33 L1
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