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AB85273

Recombinant Human 14-3-3 alpha + beta protein

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Recombinant Human 14-3-3 alpha + beta protein is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.

View Alternative Names

14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB

1 Images
SDS-PAGE - Recombinant Human 14-3-3 alpha + beta protein (AB85273)
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SDS-PAGE - Recombinant Human 14-3-3 alpha + beta protein (AB85273)

SDS-PAGE showing ab85273 at approximately 29kDa.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

P31946

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.29% Sodium chloride, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P31946","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

14-3-3 alpha + beta proteins also known as YWHAZ (14-3-3 zeta) and YWHAB (14-3-3 beta) are part of a family of conserved proteins weighing approximately 28-33 kDa each. These proteins are found in many tissues and engage in binding phosphorylated serine/threonine residues on their target molecules. This binding influences the activity localization and stability of these target proteins. Expression of 14-3-3 alpha + beta proteins is prominent in the brain where they participate in key signaling pathways.
Biological function summary

14-3-3 proteins play central roles in regulating diverse cellular processes. These proteins frequently serve as scaffolding molecules stabilizing complexes for signal transduction pathways. By interacting with numerous partner proteins 14-3-3 complexes modulate cell cycle control apoptosis and DNA damage response. They help maintain cellular homeostasis by binding and regulating enzymes receptors and transcription factors.

Pathways

14-3-3 proteins are integral to many signaling cascades. The MAPK/ERK pathway involves these proteins with 14-3-3 binding to Raf kinases influencing signal transduction. They also participate in the PI3K/Akt pathway where 14-3-3 interaction with Akt affects cell survival and growth. 14-3-3 proteins regulate various cellular functions by interacting with related kinases and phosphatases in these pathways.

Alterations of 14-3-3 protein function have significant implications. Neurodegenerative disorders such as Alzheimer's disease show changes in 14-3-3 protein binding patterns affecting neuronal stability. Aberrant 14-3-3 interactions also connect to cancerous pathways particularly in breast cancer where regulation of p53 and other tumor suppressor proteins becomes disrupted. 14-3-3 proteins influence disease progression through their extensive network of protein interactions.
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Specifications

Form

Liquid

Additional notes

Purity was determined to be >90% by densitometry.

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General info

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.

Sequence similarities

Belongs to the 14-3-3 family.

Post-translational modifications

The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.

Subcellular localisation

Vacuole membrane

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Product protocols

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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
See full target information YWHAB phospho S184 + S186
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