Recombinant Human 14-3-3 eta/YWHAH protein is a Human Full Length protein, in the 1 to 246 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G D R E Q L L Q R A R L A E Q A E R Y D D M A S A M K A V T E L N E P L S N E D R N L L S V A Y K N V V G A R R S S W R V I S S I E Q K T M A D G N E K K L E K V K A Y R E K I E K E L E T V C N D V L S L L D K F L I K N C N D F Q Y E S K V F Y L K M K G D Y Y R Y L A E V A S G E K K N S V V E A S E A A Y K E A F E I S K E Q M Q P T H P I R L G L A L N F S V F Y Y E I Q N A P E Q A C L L A K Q A F D D A I A E L D T L N E D S Y K D S T L I M Q L L R D N L T L W T S D Q Q D E E A G E G N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Alternative names
YWHA1, YWHAH, 14-3-3 protein eta, Protein AS1
Recommended products
Recombinant Human 14-3-3 eta/YWHAH protein is a Human Full Length protein, in the 1 to 246 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q04917-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M G D R E Q L L Q R A R L A E Q A E R Y D D M A S A M K A V T E L N E P L S N E D R N L L S V A Y K N V V G A R R S S W R V I S S I E Q K T M A D G N E K K L E K V K A Y R E K I E K E L E T V C N D V L S L L D K F L I K N C N D F Q Y E S K V F Y L K M K G D Y Y R Y L A E V A S G E K K N S V V E A S E A A Y K E A F E I S K E Q M Q P T H P I R L G L A L N F S V F Y Y E I Q N A P E Q A C L L A K Q A F D D A I A E L D T L N E D S Y K D S T L I M Q L L R D N L T L W T S D Q Q D E E A G E G N
- Accession
- Q04917
- Protein length
- Full Length
- Predicted molecular weight
- 28.2 kDa
- Amino acids
- 1 to 246
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab210881 was purified by using conventional chromatography techniques.
General info
- Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
- Sequence similarities
Belongs to the 14-3-3 family.
- Post-translational modifications
Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as 14-3-3 eta
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 14-3-3 eta protein also known by its gene name YWHAH functions mechanically as an adapter protein involved in many cellular processes. This protein weighs approximately 28 kDa and facilitates the modulation of signaling pathways by binding to phosphoserine-containing proteins. It exhibits widespread expression with significant presence in the brain emphasizing its role in nervous system activities. The 14-3-3 eta protein acts as a hub in various signal transduction pathways influencing cell cycle regulation and apoptosis.
- Biological function summary
The 14-3-3 eta protein interacts with diverse intracellular proteins forming various complexes that influence cellular functions. It plays a pivotal role in cellular stress responses and the regulation of several metabolic processes. The protein modulates activity by altering the localization of target proteins or masking their phosphorylation sites which affects their function and stability. This regulatory capacity emphasizes the protein's impact on cell survival and metabolism.
- Pathways
The 14-3-3 eta protein integrates into key signaling networks such as the MAPK/ERK and PI3K/AKT pathways. These pathways are essential in mediating processes like cell proliferation differentiation and survival. Within these pathways 14-3-3 eta functions alongside proteins like RAF1 and AKT1 coordinating cellular responses to external stimuli. Its involvement in these pathways highlights the adaptability and versatile roles it plays in cellular signaling and homeostasis.
- Associated diseases and disorders
The 14-3-3 eta protein has associations with conditions such as rheumatoid arthritis and neurodegenerative diseases. In rheumatoid arthritis altered expression and interactions with other proteins like cytokines contribute to inflammatory processes. In neurodegenerative disorders the dysregulation of 14-3-3 eta affects proteins related to tau pathology influencing disease progression. Understanding these connections helps in comprehending the protein's role in disease mechanisms and developing therapeutic strategies.
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1 product image
SDS-PAGE - Recombinant Human 14-3-3 eta/YWHAH protein (ab210881)
15% SDS-PAGE analysis of 3μg ab210881.
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