Recombinant Human 14-3-3 gamma/YWHAG protein is a Human Full Length protein, in the 1 to 247 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M V D R E Q L V Q K A R L A E Q A E R Y D D M A A A M K N V T E L N E P L S N E E R N L L S V A Y K N V V G A R R S S W R V I S S I E Q K T S A D G N E K K I E M V R A Y R E K I E K E L E A V C Q D V L S L L D N Y L I K N C S E T Q Y E S K V F Y L K M K G D Y Y R Y L A E V A T G E K R A T V V E S S E K A Y S E A H E I S K E H M Q P T H P I R L G L A L N Y S V F Y Y E I Q N A P E Q A C H L A K T A F D D A I A E L D T L N E D S Y K D S T L I M Q L L R D N L T L W T S D Q Q D D D G G E G N N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15696159, PubMed:16511572, PubMed:36732624). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:15696159, PubMed:16511572, PubMed:36732624). Binding generally results in the modulation of the activity of the binding partner (PubMed:16511572). Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24 (PubMed:36732624). Participates in the positive regulation of NMDA glutamate receptor activity by promoting the L-glutamate secretion through interaction with BEST1 (PubMed:29121962). Reduces keratinocyte intercellular adhesion, via interacting with PKP1 and sequestering it in the cytoplasm, thereby reducing its incorporation into desmosomes (PubMed:29678907).
Alternative names
14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, YWHAG
Recommended products
Recombinant Human 14-3-3 gamma/YWHAG protein is a Human Full Length protein, in the 1 to 247 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P61981-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M V D R E Q L V Q K A R L A E Q A E R Y D D M A A A M K N V T E L N E P L S N E E R N L L S V A Y K N V V G A R R S S W R V I S S I E Q K T S A D G N E K K I E M V R A Y R E K I E K E L E A V C Q D V L S L L D N Y L I K N C S E T Q Y E S K V F Y L K M K G D Y Y R Y L A E V A T G E K R A T V V E S S E K A Y S E A H E I S K E H M Q P T H P I R L G L A L N Y S V F Y Y E I Q N A P E Q A C H L A K T A F D D A I A E L D T L N E D S Y K D S T L I M Q L L R D N L T L W T S D Q Q D D D G G E G N N
- Accession
- P61981
- Protein length
- Full Length
- Predicted molecular weight
- 28 kDa
- Amino acids
- 1 to 247
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Recombinant human 14-3-3 was expressed in E.coli and purified by using conventional chromatography techniques.
General info
- Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15696159, PubMed:16511572, PubMed:36732624). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:15696159, PubMed:16511572, PubMed:36732624). Binding generally results in the modulation of the activity of the binding partner (PubMed:16511572). Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24 (PubMed:36732624). Participates in the positive regulation of NMDA glutamate receptor activity by promoting the L-glutamate secretion through interaction with BEST1 (PubMed:29121962). Reduces keratinocyte intercellular adhesion, via interacting with PKP1 and sequestering it in the cytoplasm, thereby reducing its incorporation into desmosomes (PubMed:29678907).
- Sequence similarities
Belongs to the 14-3-3 family.
- Post-translational modifications
Phosphorylated by various PKC isozymes.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as 14-3-3 gamma.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
14-3-3 gamma also known as YWHAG or 14-3-3 gamma protein is a member of the 14-3-3 protein family. These proteins play significant roles in signal transduction. 14-3-3 gamma is a dimeric protein with a molecular mass of about 28 to 32 kDa per monomer. It is ubiquitously expressed including in tissues such as brain muscle and heart. This protein influences a wide range of cellular processes by interacting with various signaling proteins often through recognition of phosphorylated serine or threonine motifs.
- Biological function summary
Proteins within the 14-3-3 family like the 14-3-3 gamma function as adaptors and scaffolds in signal transduction pathways. They are part of multi-protein complexes that modulate interactions among proteins within the cell. 14-3-3 gamma interacts dynamically with a variety of target proteins assisting in their activities and proper localization. Its interaction facilitates cellular processes including cell cycle control apoptosis and stress response owing to its activity as a regulator of distinct signaling pathways.
- Pathways
14-3-3 gamma participates in the MAPK/ERK pathway which is pivotal for cell growth and differentiation. It also plays an integral role in the PI3K/AKT pathway which contributes to survival and proliferation signals. In these pathways 14-3-3 gamma interacts with important kinases such as Raf and AKT. These interactions help transduce signals from receptors on the cell surface to the nucleus impacting gene expression and cellular responses.
- Associated diseases and disorders
Research has linked 14-3-3 gamma to neurodegenerative diseases such as Alzheimer's disease. In Alzheimer's 14-3-3 gamma interacts with proteins like tau which become abnormally phosphorylated and aggregate. Additionally this protein has associations with certain cancers where it might influence tumor growth and progression through interactions with cell cycle regulatory proteins. Its modulation of key signaling pathways makes it a candidate of interest in the development of therapeutic strategies for these diseases.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human 14-3-3 gamma/YWHAG protein (ab53869)
3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com