Recombinant Human 14-3-3 sigma/SFN protein (Tagged) is a Human Full Length protein, in the 1 to 248 aa range, expressed in Escherichia coli, with >94% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >94% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M E R A S L I Q K A K L A E Q A E R Y E D M A A F M K G A V E K G E E L S C E E R N L L S V A Y K N V V G G Q R A A W R V L S S I E Q K S N E E G S E E K G P E V R E Y R E K V E T E L Q G V C D T V L G L L D S H L I K E A G D A E S R V F Y L K M K G D Y Y R Y L A E V A T G D D K K R I I D S A R S A Y Q E A M D I S K K E M P P T N P I R L G L A L N F S V F H Y E I A N S P E E A I S L A K T T F D E A M A D L H T L S E D S Y K D S T L I M Q L L R D N L T L W T A D N A G E E G G E A P Q E P Q S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binding generally results in the modulation of the activity of the binding partner (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response (PubMed:37797010). Also acts as a TP53/p53-regulated inhibitor of G2/M progression (PubMed:9659898). When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis (PubMed:24124604). Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion (PubMed:29678907). May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53 (PubMed:18382127).
Alternative names
HME1, SFN, 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin
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Recombinant Human 14-3-3 sigma/SFN protein (Tagged) is a Human Full Length protein, in the 1 to 248 aa range, expressed in Escherichia coli, with >94% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >94% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P31947-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.31% Glutathione, 0.24% Tris
Sequence info
Amino acid sequence
- M E R A S L I Q K A K L A E Q A E R Y E D M A A F M K G A V E K G E E L S C E E R N L L S V A Y K N V V G G Q R A A W R V L S S I E Q K S N E E G S E E K G P E V R E Y R E K V E T E L Q G V C D T V L G L L D S H L I K E A G D A E S R V F Y L K M K G D Y Y R Y L A E V A T G D D K K R I I D S A R S A Y Q E A M D I S K K E M P P T N P I R L G L A L N F S V F H Y E I A N S P E E A I S L A K T T F D E A M A D L H T L S E D S Y K D S T L I M Q L L R D N L T L W T A D N A G E E G G E A P Q E P Q S
- Accession
- P31947
- Protein length
- Full Length
- Predicted molecular weight
- 50.1 kDa
- Amino acids
- 1 to 248
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binding generally results in the modulation of the activity of the binding partner (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response (PubMed:37797010). Also acts as a TP53/p53-regulated inhibitor of G2/M progression (PubMed:9659898). When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis (PubMed:24124604). Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion (PubMed:29678907). May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53 (PubMed:18382127).
- Sequence similarities
Belongs to the 14-3-3 family.
- Post-translational modifications
Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 14-3-3 sigma protein also known as SFN is a member of the 14-3-3 family of proteins. It plays a major role in cellular processes by regulating various signaling pathways. This protein has a molecular mass of about 28 kDa. The expression of 14-3-3 sigma is most notable in epithelial cells and it participates in cell cycle control. It interacts with a wide range of signaling molecules emphasizing its involvement in cellular regulation.
- Biological function summary
14-3-3 sigma contributes to cell cycle arrest and apoptosis. As part of a complex it interacts with other proteins to ensure proper cell division and prevent abnormal growth. It binds to phosphorylated serine/threonine residues on its target proteins influencing their activity and function. By doing this it exerts control over cell cycle checkpoints and influences DNA damage response mechanisms.
- Pathways
14-3-3 sigma interacts with several pathways that are critical for maintaining cell stability and response to stress. It participates in the PI3K/AKT pathway working closely with proteins like AKT1 to facilitate cell survival and growth suppression under stress conditions. Its involvement in this pathway ties it to the regulation of cell proliferation and survival interconnecting with broader cellular networks.
- Associated diseases and disorders
Disruptions in 14-3-3 sigma function are linked to cancer and neurodegenerative diseases. In cancer its downregulation or loss leads to unchecked cell division contributing to tumor progression. In neurodegenerative diseases such as Alzheimer's the protein's interaction with tau phosphorylation processes affects disease progression. Furthermore it interacts with proteins like p53 emphasizing its role in tumor suppression and cellular stress responses.
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SDS-PAGE - Recombinant Human 14-3-3 sigma/SFN protein (Tagged) (ab276325)
SDS-PAGE analysis of ab276325
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