Recombinant Human 14-3-3 Theta + Tau protein is a Human Full Length protein, in the 1 to 245 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >80%
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A S M T G G Q Q M G R G S M E K T E L I Q K A K L A E Q A E R Y D D M A T C M K A V T E Q G A E L S N E E R N L L S V A Y K N V V G G R R S A W R V I S S I E Q K T D T S D K K L Q L I K D Y R E K V E S E L R S I C T T V L E L L D K Y L I A N A T N P E S K V F Y L K M K G D Y F R Y L A E V A C G D D R K Q T I D N S Q G A Y Q E A F D I S K K E M Q P T H P I R L G L A L N F S V F Y Y E I L N N P E L A C T L A K T A F D E A I A E L D T L N E D S Y K D S T L I M Q L L R D N L T L W T S D S A G E E C D A A E G A E N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Alternative names
14-3-3 protein theta, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ
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Recombinant Human 14-3-3 Theta + Tau protein is a Human Full Length protein, in the 1 to 245 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >80%
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P27348-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.6% HEPES, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.012% Benzamidine, 0.004% PMSF
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A S M T G G Q Q M G R G S M E K T E L I Q K A K L A E Q A E R Y D D M A T C M K A V T E Q G A E L S N E E R N L L S V A Y K N V V G G R R S A W R V I S S I E Q K T D T S D K K L Q L I K D Y R E K V E S E L R S I C T T V L E L L D K Y L I A N A T N P E S K V F Y L K M K G D Y F R Y L A E V A C G D D R K Q T I D N S Q G A Y Q E A F D I S K K E M Q P T H P I R L G L A L N F S V F Y Y E I L N N P E L A C T L A K T A F D E A I A E L D T L N E D S Y K D S T L I M Q L L R D N L T L W T S D S A G E E C D A A E G A E N
- Accession
- P27348
- Protein length
- Full Length
- Predicted molecular weight
- 31 kDa
- Amino acids
- 1 to 245
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab116444 was purified via Ni2+-NTA agarose columns.
General info
- Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
- Sequence similarities
Belongs to the 14-3-3 family.
- Post-translational modifications
Ser-232 is probably phosphorylated by CK1.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab116444 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF mass spectrometry. The peptides obtained gave 64 % sequence coverage of 14-3-3 tau + theta. Theoretical pI: 5.2
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
14-3-3 Theta also known as 14-3-3 protein theta is a member of the 14-3-3 protein family which consists of seven distinct isoforms in humans. It acts as an adaptor or scaffold aiding in the regulation of signal transduction pathways by binding to phosphoserine-containing proteins. The protein typically has a molecular mass of approximately 28-32 kDa. It is abundantly expressed in various tissues with a high concentration in the brain where it interacts with a number of neuronal proteins.
- Biological function summary
14-3-3 Theta plays a significant role in cell cycle regulation apoptosis and brain development. It often forms part of larger protein complexes acting as a bridge to facilitate interactions between different proteins. The protein aids in maintaining cellular homeostasis and mediates various signal transduction processes by changing the conformation of its binding partners which influences their activity.
- Pathways
14-3-3 Theta is actively involved in the MAPK/ERK and PI3K/AKT pathways which are critical for cell proliferation differentiation and survival. Through these pathways the protein interacts with a variety of partners including thr-308-phosphorylated Akt and Raf kinases. 14-3-3 Theta's ability to bind different phosphorylated targets enables it to influence diverse cellular processes within these pathways.
- Associated diseases and disorders
14-3-3 Theta has been linked to neurodegenerative diseases such as Alzheimer's and Parkinson's. Notably its interaction with tau protein plays an important role in Alzheimer's disease as abnormal phosphorylation and aggregation of tau is a characteristic of the disorder. Furthermore disruptions in 14-3-3 Theta function have connections to cancer by interacting with signaling proteins like the tumor suppressor p53 leading to altered cell growth and survival.
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1 product image
SDS-PAGE - Recombinant Human 14-3-3 Theta + Tau protein (ab116444)
12% SDS-PAGE gel showing ab116444.
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