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AB85268

Recombinant Human 14-3-3 zeta protein

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(1 Publication)

Recombinant Human 14-3-3 zeta protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ

3 Images
Western blot - Recombinant Human 14-3-3 zeta protein (AB85268)
  • WB

Unknown

Western blot - Recombinant Human 14-3-3 zeta protein (AB85268)

All lanes:

Western blot - Anti-14-3-3 zeta antibody (<a href='/en-us/products/primary-antibodies/14-3-3-zeta-antibody-ab51129'>ab51129</a>) at 1/500 dilution

Lane 1:

Western blot - Recombinant Human 14-3-3 zeta protein (ab85268) at 0.1 µg

Lane 2:

Western blot - Recombinant Human 14-3-3 zeta protein (ab85268) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 28 kDa

true

Exposure time: 4min

Western blot - Recombinant Human 14-3-3 zeta protein (AB85268)
  • WB

Unknown

Western blot - Recombinant Human 14-3-3 zeta protein (AB85268)

All lanes:

Western blot - Anti-14-3-3 zeta antibody (<a href='/en-us/products/primary-antibodies/14-3-3-zeta-antibody-ab51129'>ab51129</a>) at 1/500 dilution

Lane 1:

Western blot - Recombinant Human 14-3-3 zeta protein (ab85268) at 0.1 µg

Lane 2:

Western blot - Recombinant Human 14-3-3 zeta protein (ab85268) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 28 kDa

true

Exposure time: 4min

SDS-PAGE - Recombinant Human 14-3-3 zeta protein (AB85268)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human 14-3-3 zeta protein (AB85268)

SDS-PAGE showing ab85268 at approximately 29kDa.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P63104

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.29% Sodium chloride, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab85268 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/14-3-3-zeta-antibody-ab51129'>ab51129</a>.</p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P63104","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 zeta protein also known as 14-3-3 protein zeta/delta and 1433 protein belongs to the 14-3-3 protein family. These proteins have a molecular weight of around 28-33 kDa. 14-3-3 zeta proteins are expressed across various human tissues including the brain heart and muscles. Mechanically the 14-3-3 zeta protein interacts with different phosphorylated serine and threonine residues on its target proteins. This binding modulates the target proteins' conformation and activity influencing their function in cellular signaling and regulation.
Biological function summary

The 14-3-3 zeta protein plays an important role in several cellular processes. It acts as an adapter in signal transduction pathways and cell cycle control. Additionally the 14-3-3 zeta protein forms complexes with other proteins to regulate apoptosis and stress responses. By binding to various partners this protein influences the distribution and localization of proteins within the cell affecting diverse functions such as metabolism and cell growth.

Pathways

The 14-3-3 zeta protein participates actively in two primary pathways: the MAPK/ERK and PI3K/AKT pathways. Within the MAPK/ERK pathway it influences cellular differentiation and proliferation interacting closely with proteins like Raf and MEK. In the PI3K/AKT pathway 14-3-3 zeta affects cell survival working alongside proteins such as AKT and Bad. These interactions make 14-3-3 zeta a significant player in the regulation of cellular processes.

14-3-3 zeta protein has been connected with neurodegenerative diseases and cancer. Altered expression or function of this protein has been observed in conditions like Alzheimer's disease where it interacts with tau protein and in various cancers where 14-3-3 zeta impacts tumor progression by modulating the function of proteins like p53 and Rb. Understanding these interactions helps in exploring therapeutic strategies for disease intervention.
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Specifications

Form

Liquid

Additional notes

Purity was determined to be >95% by densitometry.

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General info

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 14578935, PubMed : 15071501, PubMed : 15644438, PubMed : 16376338, PubMed : 16959763, PubMed : 31024343, PubMed : 9360956). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 35662396). Binding generally results in the modulation of the activity of the binding partner (PubMed : 35662396). Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB (PubMed : 35662396). Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation (PubMed : 16959763). In neurons, regulates spine maturation through the modulation of ARHGEF7 activity (By similarity).

Sequence similarities

Belongs to the 14-3-3 family.

Post-translational modifications

The delta, brain-specific form differs from the zeta form in being phosphorylated (Probable). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria (PubMed:15071501, PubMed:15696159). Phosphorylation on Thr-232; inhibits binding of RAF1 (PubMed:9360956). Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion (PubMed:11956222, PubMed:12865427, PubMed:15883165, PubMed:16376338). Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53 (PubMed:11956222, PubMed:12865427, PubMed:15883165, PubMed:16376338).

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Product protocols

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Target data

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 14578935, PubMed : 15071501, PubMed : 15644438, PubMed : 16376338, PubMed : 16959763, PubMed : 31024343, PubMed : 9360956). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 35662396). Binding generally results in the modulation of the activity of the binding partner (PubMed : 35662396). Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB (PubMed : 35662396). Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation (PubMed : 16959763). In neurons, regulates spine maturation through the modulation of ARHGEF7 activity (By similarity).
See full target information 14-3-3 protein zeta/delta
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastroenterology 28:3869-3885 PubMed36157541

2022

N-linked glycoproteomic profiling in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Wei Liu,Hao-Jie Ruan,Wei-Xia Chao,Meng-Xiang Li,Ye-Lin Jiao,Douglas G Ward,She-Gan Gao,Yi-Jun Qi
View all publications
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Complementary Products

Primary Antibodies

AB317439

Anti-14-3-3 zeta antibody [EPR27044-19] - BSA and Azide free

Recombinant|RabMAb

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody [EPR27044-19] - BSA and Azide free (AB317439)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB157201

Anti-Raf1 (phospho S621) antibody [EPR1521(2)]

RabMAb|Recombinant|Lab Essentials

Western blot - Anti-Raf1 (phospho S621) antibody [EPR1521(2)] (AB157201)

  • 0
  • 0
Primary Antibodies

AB215715

Anti-TGF beta 1 antibody [EPR21143]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-TGF beta 1 antibody [EPR21143] (AB215715)

  • 5
  • 6

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