Recombinant Human 14-3-3 zeta protein is a Human Full Length protein, in the 1 to 245 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
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- Journal of experimental & clinical cancer research : CR 42:452023Procoxacin bidirectionally inhibits osteoblastic and osteoclastic activity in bone and suppresses bone metastasis of prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDepei Kong,Chen Ye,Chenxi Zhang,Xiaochen Sun,Fubo Wang,Rui Chen,Guangan Xiao,Shipeng He,Jianrong Xu,Xiwu Rao,Jianzhong Ai,Xu Gao,Hong Li,Li SuPubMed 36759880
- Communications biology 4:12212021PIM1 phosphorylation of the androgen receptor and 14-3-3 ζ regulates gene transcription in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophie E Ruff,Nikita Vasilyev,Evgeny Nudler,Susan K Logan,Michael J GarabedianPubMed 34697370
- Oncology letters 16:1815-18202018Effect of cytokine-induced killer cells combined with dendritic cells on the survival rate and expression of 14-3-3ζ and p-Bad proteins in Lewis lung cancer cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Hou et. al.PubMed 30008870
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S H M D K N E L V Q K A K L A E Q A E R Y D D M A A C M K S V T E Q G A E L S N E E R N L L S V A Y K N V V G A R R S S W R V V S S I E Q K T E G A E K K Q Q M A R E Y R E K I E T E L R D I C N D V L S L L E K F L I P N A S Q A E S K V F Y L K M K G D Y Y R Y L A E V A A G D D K K G I V D Q S Q Q A Y Q E A F E I S K K E M Q P T H P I R L G L A L N F S V F Y Y E I L N S P E K A C S L A K T A F D E A I A E L D T L S E E S Y K D S T L I M Q L L R D N L T L W T S D T Q G D E A E A G E G G E N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:14578935, PubMed:15071501, PubMed:15644438, PubMed:16376338, PubMed:16959763, PubMed:31024343, PubMed:9360956). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:35662396). Binding generally results in the modulation of the activity of the binding partner (PubMed:35662396). Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB (PubMed:35662396). Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation (PubMed:16959763). In neurons, regulates spine maturation through the modulation of ARHGEF7 activity (By similarity).
Alternative names
14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ
Recommended products
Recombinant Human 14-3-3 zeta protein is a Human Full Length protein, in the 1 to 245 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P63104-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS
Sequence info
Amino acid sequence
- M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S H M D K N E L V Q K A K L A E Q A E R Y D D M A A C M K S V T E Q G A E L S N E E R N L L S V A Y K N V V G A R R S S W R V V S S I E Q K T E G A E K K Q Q M A R E Y R E K I E T E L R D I C N D V L S L L E K F L I P N A S Q A E S K V F Y L K M K G D Y Y R Y L A E V A A G D D K K G I V D Q S Q Q A Y Q E A F E I S K K E M Q P T H P I R L G L A L N F S V F Y Y E I L N S P E K A C S L A K T A F D E A I A E L D T L S E E S Y K D S T L I M Q L L R D N L T L W T S D T Q G D E A E A G E G G E N
- Accession
- P63104
- Protein length
- Full Length
- Predicted molecular weight
- 32 kDa
- Amino acids
- 1 to 245
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purified by using conventional chromatography techniques.
General info
- Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:14578935, PubMed:15071501, PubMed:15644438, PubMed:16376338, PubMed:16959763, PubMed:31024343, PubMed:9360956). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:35662396). Binding generally results in the modulation of the activity of the binding partner (PubMed:35662396). Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB (PubMed:35662396). Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation (PubMed:16959763). In neurons, regulates spine maturation through the modulation of ARHGEF7 activity (By similarity).
- Sequence similarities
Belongs to the 14-3-3 family.
- Post-translational modifications
The delta, brain-specific form differs from the zeta form in being phosphorylated (Probable). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria (PubMed:15071501, PubMed:15696159). Phosphorylation on Thr-232; inhibits binding of RAF1 (PubMed:9360956). Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion (PubMed:11956222, PubMed:12865427, PubMed:15883165, PubMed:16376338). Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53 (PubMed:11956222, PubMed:12865427, PubMed:15883165, PubMed:16376338).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C, -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 14-3-3 zeta protein also known as 14-3-3 protein zeta/delta and 1433 protein belongs to the 14-3-3 protein family. These proteins have a molecular weight of around 28-33 kDa. 14-3-3 zeta proteins are expressed across various human tissues including the brain heart and muscles. Mechanically the 14-3-3 zeta protein interacts with different phosphorylated serine and threonine residues on its target proteins. This binding modulates the target proteins' conformation and activity influencing their function in cellular signaling and regulation.
- Biological function summary
The 14-3-3 zeta protein plays an important role in several cellular processes. It acts as an adapter in signal transduction pathways and cell cycle control. Additionally the 14-3-3 zeta protein forms complexes with other proteins to regulate apoptosis and stress responses. By binding to various partners this protein influences the distribution and localization of proteins within the cell affecting diverse functions such as metabolism and cell growth.
- Pathways
The 14-3-3 zeta protein participates actively in two primary pathways: the MAPK/ERK and PI3K/AKT pathways. Within the MAPK/ERK pathway it influences cellular differentiation and proliferation interacting closely with proteins like Raf and MEK. In the PI3K/AKT pathway 14-3-3 zeta affects cell survival working alongside proteins such as AKT and Bad. These interactions make 14-3-3 zeta a significant player in the regulation of cellular processes.
- Associated diseases and disorders
14-3-3 zeta protein has been connected with neurodegenerative diseases and cancer. Altered expression or function of this protein has been observed in conditions like Alzheimer's disease where it interacts with tau protein and in various cancers where 14-3-3 zeta impacts tumor progression by modulating the function of proteins like p53 and Rb. Understanding these interactions helps in exploring therapeutic strategies for disease intervention.
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SDS-PAGE - Recombinant Human 14-3-3 zeta protein (ab87361)
ab87361 on 15% SDS-PAGE (3μg)
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