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AB132282

Recombinant Human 15-LOX-2 protein (GST tag N-Terminus)

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Recombinant Human 15-LOX-2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 676 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Polyunsaturated fatty acid lipoxygenase ALOX15B, 15-lipoxygenase 2, Arachidonate 15-lipoxygenase B, Arachidonate 15-lipoxygenase type II, Linoleate 13-lipoxygenase 15-LOb, 15-LOX-2, 15-LOX-B, ALOX15B

1 Images
SDS-PAGE - Recombinant Human 15-LOX-2 protein (GST tag N-Terminus) (AB132282)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human 15-LOX-2 protein (GST tag N-Terminus) (AB132282)

12.5% SDS-PAGE stained with Coomassie Blue showing ab132282.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

O15296

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Proteins & Peptides

AB196053

Recombinant Human 15 Lipoxygenase 1 protein (DDDDK tag N-Terminus)

SDS-PAGE - Recombinant Human 15 Lipoxygenase 1 protein (DDDDK tag N-Terminus) (AB196053)

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  • 0
Proteins & Peptides

AB198646

Recombinant human COX2 / Cyclooxygenase 2 protein (DDDDK tag C-Terminus + His tag C-Terminus)

Functional Studies - Recombinant human COX2 / Cyclooxygenase 2 protein (DDDDK tag C-Terminus + His tag C-Terminus) (AB198646)

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Primary Antibodies

AB244205

Anti-15 Lipoxygenase 1 antibody [EPR22138]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (AB244205)

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  • 0
Primary Antibodies

AB168384

Anti-12 Lipoxygenase/ALOX12 antibody [EPR11380(B)]

RabMAb|Recombinant

Western blot - Anti-12 Lipoxygenase/ALOX12 antibody [EPR11380(B)] (AB168384)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAEFRVRVSTGEAFGAGTWDKVSVSIVGTRGESPPLPLDNLGKEFTAGAEEDFQVTLPEDVGRVLLLRVHKAPPVLPLLGPLAPDAWFCRWFQLTPPRGGHLLFPCYQWLEGAGTLVLQEGTAKVSWADHHPVLQQQRQEELQARQEMYQWKAYNPGWPHCLDEKTVEDLELNIKYSTAKNANFYLQAGSAFAEMKIKGLLDRKGLWRSLNEMKRIFNFRRTPAAEHAFEHWQEDAFFASQFLNGLNPVLIRRCHYLPKNFPVTDAMVASVLGPGTSLQAELEKGSLFLVDHGILSGIQTNVINGKPQFSAAPMTLLYQSPGCGPLLPLAIQLSQTPGPNSPIFLPTDDKWDWLLAKTWVRNAEFSFHEALTHLLHSHLLPEVFTLATLRQLPHCHPLFKLLIPHTRYTLHINTLARELLIVPGQVVDRSTGIGIEGFSELIQRNMKQLNYSLLCLPEDIRTRGVEDIPGYYYRDDGMQIWGAVERFVSEIIGIYYPSDESVQDDRELQAWVREIFSKGFLNQESSGIPSSLETREALVQYVTMVIFTCSAKHAAVSAGQFDSCAWMPNLPPSMQLPPPTSKGLATCEGFIATLPPVNATCDVILALWLLSKEPGDQRPLGTYPDEHFTEEAPRRSIATFQSRLAQISRGIQERNRGLVLPYTYLDPPLIENSVSI","proteinLength":"Full Length","predictedMolecularWeight":"102.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":676,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O15296","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

15-LOX-2 also known as 15-lipoxygenase-2 is an enzyme that catalyzes the conversion of arachidonic acid to 15-hydroperoxyicosatetraenoic acid (15-HPETE). Its molecular mass is roughly 76 kDa. 15-LOX-2 is expressed in various tissues with high presence in the prostate lungs and skin. This enzyme is also detected in certain epithelial tissues suggesting its involvement in cellular processes related to skin and prostate health.
Biological function summary

15-LOX-2 plays roles in lipid metabolism by catalyzing specific biochemical reactions. It helps in regulating inflammation through its metabolites. While not part of a larger enzyme complex 15-LOX-2 works alongside other lipoxygenases to manage lipid signaling molecules. Its function further influences cellular differentiation and apoptosis offering an important connection to tissue homeostasis and cellular turnover rates.

Pathways

15-LOX-2 contributes to the arachidonic acid metabolic pathway and eicosanoid signaling pathways. It interacts with cyclooxygenases and other lipoxygenases within these pathways. This interaction affects the balance of pro-inflammatory and anti-inflammatory mediators. The enzyme's activity relates specifically to eicosanoids like leukotrienes and prostaglandins which influence immune responses and vascular functions.

15-LOX-2 shows involvement in prostate cancer and atherosclerosis. Its regulation of inflammatory mediators suggests a role in inflammation-associated pathologies. In prostate cancer altered expression of 15-LOX-2 links to tumor progression interacting with enzymes like COX-2. In atherosclerosis dysregulation of its activity may lead to abnormal lipid metabolism and vessel inflammation demonstrating its potential as a therapeutic target.
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Specifications

Form

Liquid

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General info

Function

Isoform A. Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (Probable) (PubMed : 10542053, PubMed : 10625675, PubMed : 12704195, PubMed : 17493578, PubMed : 18311922, PubMed : 24282679, PubMed : 24497644, PubMed : 32404334, PubMed : 9177185). It inserts peroxyl groups at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (Probable) (PubMed : 10625675, PubMed : 11956198, PubMed : 12704195, PubMed : 17493578, PubMed : 24282679, PubMed : 24497644, PubMed : 9177185). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (Probable) (PubMed : 10542053, PubMed : 27435673). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist (PubMed : 11956198, PubMed : 17493578, PubMed : 18311922). Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates (PubMed : 27145229). Participates in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) resolvin D5 ((7S,17S)-diHPDHA), which can actively down-regulate the immune response and have anti-aggregation properties with platelets (PubMed : 32404334). In addition to free PUFAs hydrolyzed from phospholipids, it directly oxidizes PUFAs esterified to membrane-bound phospholipids (PubMed : 27435673). Has no detectable 8S-lipoxygenase activity on arachidonate but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE (Probable). May regulate progression through the cell cycle and cell proliferation (PubMed : 11839751, PubMed : 12704195). May also regulate cytokine secretion by macrophages and therefore play a role in the immune response (PubMed : 18067895). May also regulate macrophage differentiation into proatherogenic foam cells (PubMed : 22912809).. Isoform B. Does not convert arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE.

Sequence similarities

Belongs to the lipoxygenase family.

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Product protocols

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Target data

Isoform A. Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (Probable) (PubMed : 10542053, PubMed : 10625675, PubMed : 12704195, PubMed : 17493578, PubMed : 18311922, PubMed : 24282679, PubMed : 24497644, PubMed : 32404334, PubMed : 9177185). It inserts peroxyl groups at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (Probable) (PubMed : 10625675, PubMed : 11956198, PubMed : 12704195, PubMed : 17493578, PubMed : 24282679, PubMed : 24497644, PubMed : 9177185). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (Probable) (PubMed : 10542053, PubMed : 27435673). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist (PubMed : 11956198, PubMed : 17493578, PubMed : 18311922). Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates (PubMed : 27145229). Participates in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) resolvin D5 ((7S,17S)-diHPDHA), which can actively down-regulate the immune response and have anti-aggregation properties with platelets (PubMed : 32404334). In addition to free PUFAs hydrolyzed from phospholipids, it directly oxidizes PUFAs esterified to membrane-bound phospholipids (PubMed : 27435673). Has no detectable 8S-lipoxygenase activity on arachidonate but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE (Probable). May regulate progression through the cell cycle and cell proliferation (PubMed : 11839751, PubMed : 12704195). May also regulate cytokine secretion by macrophages and therefore play a role in the immune response (PubMed : 18067895). May also regulate macrophage differentiation into proatherogenic foam cells (PubMed : 22912809).. Isoform B. Does not convert arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE.
See full target information ALOX15B
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